Kevin L Lorick,Yili Yang,Jane P Jensen et al.
Kevin L Lorick et al.
A concept that has arisen over the last decade is that proteins can, in general, be covalently modified by polypeptides, resulting in alterations in their fate and function. The first-identified and most well studied of these modifying poly...
Ulrich Blank,Juan Rivera
Ulrich Blank
Mast cells are important effectors in innate and adaptive immune responses. They contain numerous secretory granules filled with inflammatory mediators in their cytoplasm. Exocytosis of granular content does not take place until the cell re...
Mohamed Amessou,Vincent Popoff,Belèn Yelamos et al.
Mohamed Amessou et al.
The recently described retrograde transport route is a highly selective pathway that allows some internalized molecules to reach the trans-Golgi network from early/recycling endosomes, bypassing the recycling route to the plasma membrane an...
Joie A Bernard-Trifilo,Ssang-Taek Lim,Shihe Hou et al.
Joie A Bernard-Trifilo et al.
Integrins are a family of heterodimeric alpha/beta transmembrane cell adhesion receptors that play important roles in the regulation of cell migration, proliferation, and survival. Integrins do not possess intrinsic catalytic activity, and ...
Sally Kornbluth,Jing Yang,Maureen Powers
Sally Kornbluth
In this unit, Xenopus eggs are isolated from hormonally primed female frogs, and then the extract is treated with cyclohexamide so it remains in interphase of the cell cycle. In the presence of sperm chromatin and ATP, membrane vesicles in ...
Isolation of melanosomes [0.03%]
黑素体的分离
Hidenori Watabe,Tsuneto Kushimoto,Julio C Valencia et al.
Hidenori Watabe et al.
The methods used to purify early and late melanosomes are detailed. These methods include the use of highly pigmented cells to maximize recovery and the use of various sucrose density gradients to separate melanosome fractions based on thei...
Isolation of clathrin-coated vesicles by differential and density gradient centrifugation [0.03%]
用差速和密度梯度离心法分离网格蛋白包被小泡
Martine Girard,Patrick D Allaire,Francois Blondeau et al.
Martine Girard et al.
Clathrin-coated vesicles (CCVs) are an important class of transport organelles that mediate the endocytosis of proteins and lipids at the plasma membrane and the transport of proteins from the trans-Golgi network to the endosomal/lysosomal ...
Mouse embryonic stem cell derivation, and mouse and human embryonic stem cell culture and differentiation as embryoid bodies [0.03%]
小鼠胚胎干细胞的建系及小鼠和人胚胎干细胞以类胚体形式培养与分化
Brock J Conley,Mark Denham,Lerna Gulluyan et al.
Brock J Conley et al.
Embryonic stem (ES) cells are pluripotent cells derived from developing mouse blastocysts in vitro that maintain long-term self renewal and the capacity to give rise to all cell types in the adult body (including some extraembryonic cell ty...
Stem cells: an overview [0.03%]
干细胞研究综述
Mark Denham,Brock Conley,Fredrik Olsson et al.
Mark Denham et al.
Stem cells are specialized cells that possess a capacity to undergo self-renewal while at the same time having the ability to give rise to at least one or more differentiated or mature cell type. They therefore represent a fundamental corne...
Assembly and micromanipulation of Xenopus in vitro-assembled mitotic chromosomes [0.03%]
非洲爪蟾体外装配的有丝分裂染色体的组装和微操作
Sébastien Almagro,Stefan Dimitrov
Sébastien Almagro
Mitotic chromosomes have fascinated scientists for several decades. Despite the numerous microscopy studies, chromosome structure is, however, still poorly understood. This is due to both the high complexity of the mitotic chromosomes and t...