Chris I Cheeseman,Debbie O&#x;Neill
Chris I Cheeseman
This unit provides protocols for isolating intestinal brush-border membranes from rat, pig, and cow. These membranes can be used for immunoblotting or other analytical techniques. They will also spontaneously form closed vesicles which allo...
Konstantin V Kandror,Paul F Pilch
Konstantin V Kandror
The immunoisolation of GLUT4-containing vesicles from adipocytes is described in this unit. The methods involve homogenization of cells followed by differential centrifugation to provide the intracellular membranes that contain GLUT4. Subse...
Nicola Harrison-Lowe,Laura J Olsen
Nicola Harrison-Lowe
Peroxisomes are single-membrane-bound organelles found in virtually all eukaryotes. In plants, there are several classes of peroxisomes. Glyoxysomes are found in germinating seedlings and contain enzymes specific for the glyoxylate cycle, i...
Michael J Rindler
Michael J Rindler
This unit describes methods for preparing zymogen granules from rat pancreas. Zymogen granules are storage organelles in pancreatic acinar cells containing digestive enzymes that are released into the pancreatic duct. The protocols in this ...
Immunoisolation of centrosomes from Drosophila melanogaster [0.03%]
来自Drosophila melanogaster的中心体免疫隔离
Verena Lehmann,Hannah Müller,Bodo M H Lange
Verena Lehmann
Classical protocols for the isolation of centrosomes from higher eukaryotic cells are based on enrichment of cell organelles by density gradient centrifugation. Various successful protocols have been described that isolate centrosomes from ...
Ken A Lindstedt,Petri T Kovanen
Ken A Lindstedt
The mast cell is a multipotent inflammatory cell that has been shown to participate in the pathogenesis of a variety of diseases, such as immediate hypersensitivity reactions, arthritis, atherosclerosis, and heart failure. Upon stimulation,...
Dawn L Brasaemle,Nathan E Wolins
Dawn L Brasaemle
Lipid droplets are organelles found in most mammalian cells, as well as various plant tissues and yeast. They are composed of a core of neutral lipids surrounded by a membrane monolayer of phospholipids and cholesterol into which specific p...
Visualization of protein interactions in living cells using bimolecular fluorescence complementation (BiFC) analysis [0.03%]
应用双分子荧光补救技术（BiFC）在活细胞中可视化蛋白质相互作用
Chang-Deng Hu,Asya V Grinberg,Tom K Kerppola
Chang-Deng Hu
Protein interactions integrate stimuli from different signaling pathways and developmental programs. Bimolecular fluorescence complementation (BiFC) analysis has been developed for visualization of protein interactions in living cells. This...
Barbara Viviani
Barbara Viviani
The close relationship between neurons, glia, astroglia, and astrocytes plays a relevant role in modulating a neurotoxic effect and propagating the consequent degenerative event in the brain. After exposure to a neurotoxicant, glial cells a...
Rational design and evaluation of FRET experiments to measure protein proximities in cells [0.03%]
荧光共振能量转移实验的理性设计及评估以测定活细胞中蛋白间的空间距离分布
Erik L Snapp,Ramanujan S Hegde
Erik L Snapp
Fluorescence resonance energy transfer (FRET) refers to the nonradiative transfer of energy from one fluorescent molecule (the donor) to another fluorescent molecule (the acceptor). Measurement of FRET between two fluorophore-labeled protei...