David A Cruz Walma,Joshua W Collins
David A Cruz Walma
Whether screening small mammal serum during antibody production or attempting to preserve a stock of precious antibody, this protocol's western blotting method using aliquots containing nanoliter volumes of antibody will benefit researchers...
Nuclei Isolation Staining (NIS) Method for Imaging Chromatin-Associated Proteins in Difficult Cell Types [0.03%]
用于检测难以制备细胞的染色质相关蛋白的细胞核分离着色法(NIS方法)
Amy E Neely,Xiaomin Bao
Amy E Neely
Spatial distribution of chromatin-associated proteins provides invaluable information for understanding gene regulation. Conventional immunostaining is widely used for labeling chromatin-associated proteins in many cell types. However, for ...
A Neuron-Glia Co-culture System for Studying Intercellular Lipid Transport [0.03%]
用于研究细胞间脂质运输的神经胶质共培养系统
Maria S Ioannou,Zhe Liu,Jennifer Lippincott-Schwartz
Maria S Ioannou
Neurons and glia operate in a highly coordinated fashion in the brain. Although glial cells have long been known to supply lipids to neurons via lipoprotein particles, new evidence reveals that lipid transport between neurons and glia is bi...
Pinpointing the Genomic Localizations of Chromatin-Associated Proteins: The Yesterday, Today, and Tomorrow of ChIP-seq [0.03%]
染色质免疫共沉淀测序（ChIP-seq）的 yesterday、today 和 tomorrow：精准定位染色质相关蛋白质的结合位点
Sarah M Lloyd,Xiaomin Bao
Sarah M Lloyd
Chromatin-associated proteins are instrumental for controlling spatiotemporal gene expression. Determining where these proteins bind across the genome is critical for understanding gene regulation. A widely used technique at present is ChIP...
Alexandre Chojnowski,Hendrikje Werner,Matthew Cook et al.
Alexandre Chojnowski et al.
Protein-protein interactions (PPIs) add an essential layer of complexity to the information encoded by the genome. Modulation of such interactions is a key feature of most, if not all, cellular activities and allows cells to respond rapidly...
Jesse Aaron,Eric Wait,Michael DeSantis et al.
Jesse Aaron et al.
The rapid advancement of live-cell imaging technologies has enabled biologists to generate high-dimensional data to follow biological movement at the microscopic level. Yet, the "perceived" ease of use of modern microscopes has led to chall...
Preparation of Cells from Embryonic Organs for Single-Cell RNA Sequencing [0.03%]
胚胎器官细胞的单细胞RNA测序制备方法
Rei Sekiguchi,Belinda Hauser
Rei Sekiguchi
Although single-cell RNA sequencing (scRNA-seq) has become one of the most powerful methods available for transcriptome analysis, the quality of scRNA-seq data largely depends on cell preparation. Cell preparation from cultured cells and ti...
Anna-Lena Cost,Samira Khalaji,Carsten Grashoff
Anna-Lena Cost
Genetically encoded Förster resonance energy transfer (FRET)-based tension sensors measure piconewton-scale forces across individual molecules in living cells or whole organisms. These biosensors show comparably high FRET efficiencies in t...
Bioluminescence Resonance Energy Transfer 2 (BRET2)-Based RAS Biosensors to Characterize RAS Inhibitors [0.03%]
基于生物发光共振能量转移的RAS生物传感器用于表征RAS抑制剂
Nicolas Bery,Terence H Rabbitts
Nicolas Bery
Protein-protein interactions (PPIs) are principle biological processes that control normal cell growth, differentiation, and homeostasis but are also crucial in diseases such as malignancy, neuropathy, and infection. Despite the importance ...
Minghan Hu
Minghan Hu
Visualization of dynamic cellular activity has greatly expanded our understanding of brain function. Recently, there has been an increasing number of studies imaging rodent brain activity in real time. However, traditional in vivo calcium i...