Identification and analysis of multiprotein complexes through chemical crosslinking [0.03%]
通过化学交联鉴定和分析多蛋白复合物
Karsten Melcher,Hung-Ta Chen
Karsten Melcher
Chemical crosslinking provides information about protein-protein interactions in the context of intact protein complexes; therefore, it is particularly suited to the analysis of multiprotein complexes. Rather than a single distinct techniqu...
Laurent Taupenot
Laurent Taupenot
The catecholamine-secreting PC12 cell line derived from the rat adrenal medulla has long been considered a model system for neurosecretion and neuronal differentiation. PC12 cells contain a large number of secretory granules (otherwise know...
Analyzing real-time video microscopy: the dynamics and geometry of vesicles and tubules in endocytosis [0.03%]
实时视频显微镜分析：内吞作用中囊泡和管状小体的动力学及几何特征
Nicholas Hamilton,Markus C Kerr,Kevin Burrage et al.
Nicholas Hamilton et al.
With the advent of live cell imaging microscopy, new types of mathematical analyses and measurements are possible. Many of the real-time movies of cellular processes are visually very compelling, but elementary analysis of changes over time...
Rosemary A Stuart,Carla M Koehler
Rosemary A Stuart
This unit describes methods for importing in vitro-translated or recombinant proteins into isolated yeast mitochondria and for exporting mitochondrial proteins translated in the yeast mitochondrial matrix into the inner mitochondrial membra...
Preparation of extracellular matrices produced by cultured and primary fibroblasts [0.03%]
培养细胞和原代成纤维细胞产生的细胞外基质的制备
Dorothy A Beacham,Michael D Amatangelo,Edna Cukierman
Dorothy A Beacham
Fibroblasts secrete and organize extracellular matrix (ECM), which provides structural support for their adhesion, migration, and tissue organization, besides regulating cellular functions such as growth and survival. Cell-to-matrix interac...
Mary C Phelan
Mary C Phelan
Cultured mammalian cells are used extensively in cell biology studies. It requires a number of special skills in order to be able to preserve the structure, function, behavior, and biology of the cells in culture. This unit describes the ba...
Peter J Peters,Erik Bos,Alexander Griekspoor
Peter J Peters
This unit describes subcellular localization of proteins/antigens using high-resolution cryo-immunogold electron microscopy, which allows study of topological biochemistry at the ultrastructural level. This is the most sensitive procedure f...
Antonio Zorzano,Marta Camps
Antonio Zorzano
The transverse tubules (T-tubules) of mammalian cardiac and skeletal muscles are invaginations of the sarcolemma. They play a crucial role in excitation-contraction coupling as well as in intracellular signaling and in regulation of glucose...
Conrad L Leung,Ronald K H Liem
Conrad L Leung
Intermediate filaments (IFs) are found in most eukaryotic cells and are made up of various IF proteins. IFs are highly insoluble in conventional extraction buffers and are therefore commonly purified under denaturing condition. Purified IF ...
Isolation and characterization of exosomes from cell culture supernatants and biological fluids [0.03%]
从细胞培养上清液和生物体液中分离及表征外泌体
Clotilde Théry,Sebastian Amigorena,Graça Raposo et al.
Clotilde Théry et al.
Exosomes are small membrane vesicles found in cell culture supernatants and in different biological fluids. Exosomes form in a particular population of endosomes, called multivesicular bodies (MVBs), by inward budding into the lumen of the ...