Using fluorescent sphingolipid analogs to study intracellular lipid trafficking [0.03%]
利用荧光鞘脂类似物研究细胞内脂质转运
Raman Deep Singh,David L Marks,Richard E Pagano
Raman Deep Singh
Sphingolipids (SLs), including glycosphingolipids, are found on the plasma membrane where they play important roles in a wide variety of cell functions, including cell-cell communication, cell growth and differentiation, host-pathogen inter...
Malkiel A Cohen,Pavey Itsykson,Benjamin E Reubinoff
Malkiel A Cohen
Human embryonic stem cells (hESCs) may be converted into highly enriched cultures of neural precursors under defined culture conditions. The neural precursors can proliferate in culture for prolonged periods of time, and can differentiate i...
Maxim A Vodyanik,Igor I Slukvin
Maxim A Vodyanik
Human embryonic stem cells (hESCs) represent a unique population of cells capable of self-renewal and differentiation into all types of somatic cells, including hematopoietic and endothelial cells. Since the pattern of hematopoietic and end...
Jan Kramer,Peter Schlenke,Jürgen Rohwedel
Jan Kramer
This unit describes the protocols used for cultivation of murine embryonic stem (ES) cells and their differentiation into chondrogenic cell types in vitro. ES cells cultivated as cellular aggregates, so-called embryoid bodies (EBs), differe...
Differentiation of mouse embryonic stem cells and of human adult stem cells into adipocytes [0.03%]
小鼠胚胎干细胞和人类成体干细胞向脂肪细胞分化
Brigitte Wdziekonski,Phi Villageois,Christian Dani
Brigitte Wdziekonski
The authors describe protocols for culture conditions in which mouse ES cells can be maintained in an undifferentiated state or committed to undergo adipocyte differentiation at a high rate and in a highly reproducible fashion. There is als...
Maintenance and in vitro differentiation of mouse embryonic stem cells to form blood vessels [0.03%]
小鼠胚胎干细胞的维持及其体外分化形成血管细胞的研究
Nicholas C Kappas,Victoria L Bautch
Nicholas C Kappas
Embryonic stem (ES) cells, which are derived from developing mouse blastocysts, have the capacity to give rise to all cell types in the adult body. The ability of ES cells to do so has opened the door for novel experimental approaches in th...
Tomoki Yokochi,David M Gilbert
Tomoki Yokochi
In this unit, several basic protocols to identify sites of DNA replication utilizing incorporation of halogenated thymidine analogs into DNA, followed by immunofluorescent imaging are described. Antibodies specific for halogenated thymidine...
Scott G Olenych,Nathan S Claxton,Gregory K Ottenberg et al.
Scott G Olenych et al.
Advances in fluorescent protein development over the past 10 years have led to fine-tuning of the Aequorea victoria jellyfish color palette in the emission color range from blue to yellow, while a significant amount of progress has been ach...
Dmitriy Kedrin,Jeffrey Wyckoff,Erik Sahai et al.
Dmitriy Kedrin et al.
This unit describes the methods that we have been developing for analyzing tumor cell motility in mouse and rat models of breast cancer metastasis. Rodents are commonly used to provide a mammalian system for studying human tumor cells as xe...
Visualization of RNA using fluorescence complementation triggered by aptamer-protein interactions (RFAP) in live bacterial cells [0.03%]
利用活细菌细胞中的适配体-蛋白质相互作用触发的荧光补缺技术可视化RNA(RFAP)
Maria Valencia-Burton,Natalia E Broude
Maria Valencia-Burton
This unit describes a method allowing RNA visualization in live cells. The method is based on fluorescent protein complementation regulated by RNA-aptamer/RNA-binding protein interactions. Based on these two principles, a fluorescent ribonu...