Use of hyaluronan-derived hydrogels for three-dimensional cell culture and tumor xenografts [0.03%]
透明质酸衍生成胶用于三维细胞培养和异种移植肿瘤模型
Monica A Serban,Anna Scott,Glenn D Prestwich
Monica A Serban
The practice of in vitro three-dimensional (3-D) cell culture has lagged behind the realization that classical two-dimensional (2-D) culture on plastic surfaces fails to mirror normal cell biology. Biologically, a complex network of protein...
Visualizing protease activity in living cells: from two dimensions to four dimensions [0.03%]
从二维到四维：活细胞蛋白酶活性的可视化技术
Christopher Jedeszko,Mansoureh Sameni,Mary B Olive et al.
Christopher Jedeszko et al.
Proteolytic degradation of extracellular matrix (ECM) components by cells is an important metabolic activity as cells grow, remodel, and migrate through the ECM. The ability to analyze ECM degradation can be valuable in the study of develop...
Quantitative colocalization analysis of confocal fluorescence microscopy images [0.03%]
共焦荧光显微图像的定量同域分析方法研究
Vadim Zinchuk,Olga Zinchuk
Vadim Zinchuk
Colocalization is an important finding in many cell biological studies. This unit describes a protocol for quantitative evaluation of images with colocalization based on the calculation of a number of specialized coefficients. First, images...
Fluorescence imaging techniques for studying Drosophila embryo development [0.03%]
用于研究果蝇胚胎发育的荧光成像技术
Manos Mavrakis,Richa Rikhy,Mary Lilly et al.
Manos Mavrakis et al.
This unit describes fluorescence-based techniques for noninvasive imaging of development in living Drosophila embryos, discussing considerations for fluorescent imaging within living embryos and providing protocols for generation of flies e...
J Avila,H Soares,M L Fanarraga et al.
J Avila et al.
This unit describes various protocols for the isolation and purification of the main constituents of microtubules, chiefly alpha- and beta-tubulin, and the most significant microtubule associated proteins (MAPs), specifically MAP1A, MAP1B, ...
Visualization and measurement of DNA methyltransferase activity in living cells [0.03%]
活细胞中DNA甲基转移酶活性的可视化与测量
Lothar Schermelleh,Fabio Spada,Heinrich Leonhardt
Lothar Schermelleh
In this unit, a live-cell assay to measure DNA (cytosine-5) methyltransferase (MTase) activity at the single-cell level is described. This method takes advantage of the irreversible binding of enzymatically active MTases to genomic DNA subs...
Dimitri G Pestov,Yevgeniya R Lapik,Lester F Lau
Dimitri G Pestov
The synthesis of ribosomes is a major metabolic activity critical for cell growth and homeostasis. Understanding the mechanisms of ribosome biogenesis has important implications for studying both protein synthesis and cell cycle control. Th...
Wolfgang W A Schamel
Wolfgang W A Schamel
Multiprotein complexes play crucial roles in nearly all cell biological processes. Blue Native Polyacrylamide Gel Electrophoresis (BN-PAGE) is a powerful method to study these complexes. It is a native protein separation method that relies ...
Isolation of amyloplasts [0.03%]
离心分离淀粉体
Kay Denyer,Marilyn Pike
Kay Denyer
Two different methods for the preparation of starch-rich plastids are described together with protocols for the determination of plastid yield, purity, and intactness. The preparation of amyloplasts from maize endosperm and oilseed rape emb...
George H Patterson
George H Patterson
A major advance in the microscopic study of cells and tissues is the introduction of photoactivatable fluorescent proteins, which can specifically mark proteins of interest within a living cell. Fluorescent proteins are now available that a...