Naoto Haruyama,Andrew Cho,Ashok B Kulkarni
Naoto Haruyama
Cell biology research encompasses everything from single cells to whole animals. Recent discoveries concerning particular gene functions can be applied to the whole animal for understanding genotype-phenotype relationships underlying diseas...
Hari Shroff,Helen White,Eric Betzig
Hari Shroff
Key to understanding a protein's biological function is the accurate determination of its spatial distribution inside a cell. Although fluorescent protein markers allow the targeting of specific proteins with molecular precision, much of th...
Florian Tribl
Florian Tribl
Neuromelanin granules are pigmented organelles in the human midbrain that give name to a brain area, substantia nigra pars compacta, which macroscopically appears as a dark brown region in the midbrain due to the insoluble pigment neuromela...
Monitoring mRNA export [0.03%]
监控mRNA出口
Kazuaki Tokunaga,Tokio Tani
Kazuaki Tokunaga
Transport of mRNA from the nucleus to the cytoplasm is an essential process for gene expression in eukaryotic cells. In this unit, methods for monitoring nuclear mRNA export are described. Visualization of cellular mRNAs by fluorescence in ...
Mark W Carlson,Addy Alt-Holland,Christophe Egles et al.
Mark W Carlson et al.
Over the last decade, the development of in vitro, human, three-dimensional (3D) tissue models, known as human skin equivalents (HSEs), has furthered understanding of epidermal cell biology and provided novel experimental systems. Signaling...
Embryonic organ culture [0.03%]
胚胎器官培养技术
Takayoshi Sakai,Tomohiro Onodera
Takayoshi Sakai
This unit provides detailed protocols for dissecting embryonic organs and performing organ culture to study questions in developmental biology. Procedures are described here for dissecting organs such as kidney, lung, and salivary gland. Th...
Purification of intact chloroplasts from Arabidopsis and spinach leaves by isopycnic centrifugation [0.03%]
利用等密度离心法从拟南芥和菠菜叶片中分离完整的叶绿体
Daphné Seigneurin-Berny,Daniel Salvi,Jacques Joyard et al.
Daphné Seigneurin-Berny et al.
Chloroplasts are plant-specific organelles. They are the site of photosynthesis but also of many other essential metabolic pathways, such as syntheses of amino acids, vitamins, lipids, and pigments. This unit describes the isolation and pur...
Suliana Manley,Vernita D Gordon
Suliana Manley
This unit describes protocols for making giant unilamellar vesicles (GUVs) based on rehydration of dried lipid films. These model membranes are useful for determining the impact of membrane and membrane-binding components on lipid bilayer s...
Analysis of endocytic trafficking by single-cell fluorescence ratio imaging [0.03%]
单细胞荧光比率成像的内吞运输途径分析方法研究
Herve Barriere,Gergely L Lukacs
Herve Barriere
The post-endocytic sorting of internalized membrane proteins plays a critical role in numerous physiological processes, including receptor desensitization, degradation of non-native plasma membrane proteins, and cell surface retrieval of re...
Matrix metalloproteinases [0.03%]
基质金属蛋白酶
Henning Birkedal-Hansen,Susan Yamada,Jack Windsor et al.
Henning Birkedal-Hansen et al.
Matrix metalloproteinases are a class of enzymes that play an important role in the remodeling of the extracellular matrix in development and cancer metastasis. This unit describes a set of methods--cell-mediated dissolution of type-1 colla...