HIV-1 interactions with cells: from viral binding to cell-cell transmission [0.03%]
HIV-1与细胞的相互作用：从病毒结合到细胞间传播
Alicia M Janas,Li Wu
Alicia M Janas
Characterization of HIV-1 interactions with host cells is critical for cell biology studies of HIV-1. This unit describes a set of methods and protocols to perform quantitative assays of HIV-1 binding, internalization, infection, and cell-c...
Compartmented neuron cultures for directional infection by alpha herpesviruses [0.03%]
适用于α疱疹病毒定向感染的分室神经元培养系统
Dusica Curanović,Toh Hean Ch&#x;ng,Moriah Szpara et al.
Dusica Curanović et al.
Compartmented neuronal cultures allow experimenters to establish separate fluid environments for neuronal axons and the soma from which they emanate. Physical isolation of cell bodies and axons is achieved by culturing neurons in tri-chambe...
Emilio Flaño,Nancy A Jewell,Russell K Durbin et al.
Emilio Flaño et al.
This unit describes protocols for infecting the mouse respiratory tract, and assaying virus replication and host response in the lung. Respiratory infections are the leading cause of acute illness worldwide, affecting mostly infants and chi...
Alexander Eberth,Mohammad Reza Ahmadian
Alexander Eberth
Small GTPases act as tightly regulated molecular switches governing a large variety of critical cellular functions. Their activity is controlled by two different biochemical reactions, GDP/GTP exchange and GTP hydrolysis. These very slow re...
H R Petty
H R Petty
Proteins, the end product of gene expression, play a pivotal role in cellular structure and function. To understand how proteins work it is necessary to understand their physical state within the cell. This unit reviews the classification o...
Isolation of dense core secretory vesicles from pancreatic endocrine cells by differential and density gradient centrifugation [0.03%]
利用差速及密度梯度离心法从胰岛细胞中分离致密核心分泌泡
John C Hutton,Randall Wong,Howard W Davidson
John C Hutton
Methods are presented for the separation of dense core secretory vesicles from insulin-secreting tissues (insulin granules) based on a combination of differential and density gradient centrifugation on various media. Emphasis is given to th...
BK virus (BKV): infection, propagation, quantitation, purification, labeling, and analysis of cell entry [0.03%]
BK病毒(BKV)：感染、传播、定量、纯化、标记及细胞内吞分析
Takahito Moriyama,Andrey Sorokin
Takahito Moriyama
BK virus (BKV) can cause BKV nephritis in renal transplant patients and has become a significant reason for graft loss in this decade. BKV is latent in the urogenital tract and most likely is transported with the donor kidney to recipients....
Fabrication and application of nanofibrous scaffolds in tissue engineering [0.03%]
组织工程中纳米纤维支架的制备及应用研究进展
Wan-Ju Li,Rocky S Tuan
Wan-Ju Li
Nanofibers fabricated by electrospinning are morphological mimics of fibrous components of the native extracellular matrix, making nanofibrous scaffolds ideal for three-dimensional cell culture and tissue engineering applications. Although ...
Visualization of cellular phosphoinositide pools with GFP-fused protein-domains [0.03%]
带有GFP融合蛋白结构域的细胞磷脂酰肌醇池的观测研究
Tamas Balla,Péter Várnai
Tamas Balla
This unit describes the method of following phosphoinositide dynamics in live cells. Inositol phospholipids have emerged as universal signaling molecules present in virtually every membrane of eukaryotic cells. Phosphoinositides are present...
Andrew Cho,Naoto Haruyama,Ashok B Kulkarni
Andrew Cho
This unit describes detailed step-by-step protocols, reagents, and equipment required for successful generation of transgenic mice using pronuclear injection. The experimental methods and practical tips given here will help guide beginners ...