Nihal Altan-Bonnet,Grégoire Altan-Bonnet
Nihal Altan-Bonnet
Fluorescence correlation spectroscopy (FCS) is a single-molecule fluorescence technique used to monitor molecular dynamics. It can be applied to living cells expressing fluorescently labeled proteins and lipids to determine the diffusion ti...
Valarie A Barr,Stephen C Bunnell
Valarie A Barr
Interference reflection microscopy (IRM) is an optical technique used to study cell adhesion or cell mobility on a glass coverslip. The interference of reflected light waves generates images with high contrast and definition. IRM can be use...
In vivo imaging of signal transduction cascades with probes based on Förster Resonance Energy Transfer (FRET) [0.03%]
基于福斯特共振能量转移(FRET)的探针在体活细胞信号转导通路成像中的应用
Takeshi Nakamura,Michiyuki Matsuda
Takeshi Nakamura
Genetically encoded FRET probes enable us to visualize a variety of signaling events such as protein phosphorylation and G-protein activation in living cells. This unit focuses on FRET probes wherein both the donor and acceptor are fluoresc...
Andrew D Doyle
Andrew D Doyle
Micro photopatterning (microPP) has been developed to rapidly test and generate different patterns for extracellular matrix adsorption without being hindered by the process of making physical stamps through nanolithography techniques. It us...
Isolation and biochemical characterization of amyloid plaques and paired helical filaments [0.03%]
淀粉样斑块和对偶螺旋原纤维的分离与生化特征分析
Agueda Rostagno,Jorge Ghiso
Agueda Rostagno
Extracellular deposits of amyloid fibrils in the form of parenchymal plaques and cerebrovascular lesions, as well as intracellular accumulation of paired-helical filaments in the form of neurofibrillary tangles (NFT) in selected neuronal po...
Generation of gene knockout mice by ES cell microinjection [0.03%]
基因敲除小鼠的建立及显微注射法向胚胎干细胞中导入外源DNA片段的研究
Glenn Longenecker,Ashok B Kulkarni
Glenn Longenecker
This unit describes protocols used in the production of chimeric mice that are then used for the generation of gene knockout mice. These protocols include the collection of blastocyst embryos, ES cell injection, and uterine transfer of inje...
Advait Limaye,Bradford Hall,Ashok B Kulkarni
Advait Limaye
The establishment of mouse embryonic stem (ES) cell lines has allowed for the gene?ration of the knockout mouse. ES cells that are genetically altered in culture can then be manipulated to derive a whole mouse containing the desired mutatio...
Bradford Hall,Advait Limaye,Ashok B Kulkarni
Bradford Hall
The technique of gene targeting allows for the introduction of engineered genetic mutations into a mouse at a determined genomic locus. The process of generating mouse models with targeted mutations was developed through both the discovery ...
Dennis F Kucik
Dennis F Kucik
This unit describes the analysis of dynamic cell adhesion using a flow chamber assay. The flow chamber enables the researcher to reconstruct cell systems in the presence of shear stress to assay adhesion under well&defined forces. These ass...
Culturing MDCK cells in three dimensions for analyzing intracellular dynamics [0.03%]
三维培养MDCK上皮细胞以分析细胞内动态过程
Natalie Elia,Jennifer Lippincott-Schwartz
Natalie Elia
Epithelial cells grown in three-dimensional (3-D) cultures of extracellular matrix differentiate into a multicellular structure of polarized cells. This process shares many characteristics with the physiological development of an epithelial...