J C Puigvert,Hans de Bont,Bob van de Water et al.
J C Puigvert et al.
Apoptosis is important for embryonic development, tissue homeostasis, and removal of cells with (potentially transforming) DNA lesions or other types of injuries. Functional genomics screens performed to unravel apoptotic signaling cascades...
Engineering three-dimensional collagen matrices to provide contact guidance during 3D cell migration [0.03%]
工程构建三维胶原矩阵以在三维细胞迁移过程中提供接触导向
Paolo P Provenzano,Kevin W Eliceiri,David R Inman et al.
Paolo P Provenzano et al.
Cell invasion requires that cells navigate complex three-dimensional matrices in vivo. Topological cues are provided and three-dimensional cell migration and invasion facilitated by the alignment of collagen fibers proximal to tumors. In or...
Justin R Tse,Adam J Engler
Justin R Tse
The modulus of elasticity of the extracellular matrix (ECM), often referred to in a biological context as "stiffness," naturally varies within the body, e.g., hard bones and soft tissue. Moreover, it has been found to have a profound effect...
Kasturi Mitra,Jennifer Lippincott-Schwartz
Kasturi Mitra
Mitochondria are organelles that have been primarily known as the powerhouse of the cell. However, recent advances in the field have revealed that mitochondria are also involved in many other cellular activities like lipid modifications, re...
Juliane Niessen,Gabriele Jedlitschky,Andreas Greinacher et al.
Juliane Niessen et al.
Functional analysis of platelet intracellular structures requires isolation and purification of these cellular compartments. With regard to the function of platelets, both, dense (delta) and alpha granules are relevant target structures. Ho...
Isolation of Legionella-containing vacuoles by immuno-magnetic separation [0.03%]
免疫磁性分离Legionella含菌体真空室
Simon Urwyler,Ivo Finsel,Curdin Ragaz et al.
Simon Urwyler et al.
The environmental bacterium Legionella pneumophila naturally parasitizes free-living amoebae. L. pneumophila is an opportunistic human pathogen that grows in macrophages, thus causing a life-threatening pneumonia termed Legionnaires' diseas...
Methods for monitoring dynamics of pulmonary RSV replication by viral culture and by real-time reverse transcription-PCR in vivo: Detection of abortive viral replication [0.03%]
用于监测肺部分泌性呼吸道合胞病毒复制动力学的培养和实时反转录定量PCR方法：检测流产病毒复制
Marina S Boukhvalova,Kevin C Yim,Gregory A Prince et al.
Marina S Boukhvalova et al.
Viral infection is normally detected either by viral culture or by PCR methods. Rarely is a combination of the two techniques used in the same study. Yet, when applied simultaneously, viral culture and PCR may reveal important features of v...
Documenting GLUT4 exocytosis and endocytosis in muscle cell monolayers [0.03%]
肌细胞单层中GLUT4的出胞和胞吞作用的记录
Shuhei Ishikura,Costin N Antonescu,Amira Klip
Shuhei Ishikura
The elevated blood glucose following a meal is cleared by insulin-stimulated glucose entry into muscle and fat cells. The hormone increases the amount of the glucose transporter GLUT4 at the plasma membrane in these tissues at the expense o...
Quantitative analysis of endocytosis and turnover of epidermal growth factor (EGF) and EGF receptor [0.03%]
表皮生长因子及表皮生长因子受体的内吞与更新定量分析
Alexander Sorkin,Jason E Duex
Alexander Sorkin
Binding of epidermal growth factor (EGF) to the EGF receptor (EGFR) initiates signal transduction, ultimately leading to altered gene expression. Ligand-activated EGFR is also rapidly internalized and then targeted to lysosomes for degradat...
Biosensors for characterizing the dynamics of rho family GTPases in living cells [0.03%]
活细胞中Rho族GTP酶动态特性的生物传感器
Louis Hodgson,Feimo Shen,Klaus Hahn
Louis Hodgson
The biosensors developed in the authors' laboratory have been based on different designs, each imparting specific strengths and weaknesses. Here we describe detailed protocols for the application of three biosensors exemplifying different d...