Nir Drayman,Ariella Oppenheim
Nir Drayman
Traditionally, the most common methods used to titrate virus stocks are the plaque assay and the hemagglutination assay. The protocol presented here is based on the detection of viral-expressed proteins in infected cells by flow cytometry. ...
Chloé Geller,Chantal Finance,Raphaël Emmanuel Duval
Chloé Geller
Antiviral antisepsis and disinfection are crucial for preventing the environmental spread of viral infections. Emerging viruses and associated diseases, as well as nosocomial viral infections, have become a real issue in medical fields, and...
Arman Kulyyassov,Muhammad Shoaib,Vasily Ogryzko
Arman Kulyyassov
This unit describes a system for expression of biotinylated proteins in mammalian cells in vivo, and its application to chromatin immunoprecipitation (ChIP). The system is based on co-expression of the target protein fused to a short biotin...
Cell biological analysis of DT40 knockout cell lines for cell-cycle genes [0.03%]
细胞周期基因敲除DT40细胞系的细胞生物学分析
Aussie Suzuki,Tatsuo Fukagawa
Aussie Suzuki
DT40 is a chicken B cell line that has been widely used as a model for gene functional studies because the high level of homologous recombination in DT40 cells allows targeted disruption of a gene of interest. While our laboratory uses DT40...
Monitoring viral-mediated membrane fusion using fluorescent reporter methods [0.03%]
利用荧光报告基因监测病毒介导的膜融合事件
Naoyuki Kondo,Kosuke Miyauchi,Zene Matsuda
Naoyuki Kondo
A simple and real-time cell-based assay of membrane fusion employing a pair of engineered novel reporter proteins is described. The reporter proteins are chimeras of split Renilla luciferase (RL) and split green fluorescent protein (GFP). T...
Hitoshi Kuroda,Michael P Marino,Robert H Kutner et al.
Hitoshi Kuroda et al.
The use of lentiviral vectors for transgene delivery in vitro and in vivo for applications in neuroscience, hematology, developmental biology, stem cell biology, and transgenesis has become commonplace. Lentiviral vectors provide an attract...
Crystal J MacKenzie,Toshi Shioda
Crystal J MacKenzie
We present a protocol for in vitro production of recombinant lentiviruses using COS-1 African green monkey kidney epithelial cells and HEK293T human embryonic kidney epithelial cells as packaging cells. COS-1 and HEK293T express SV40 large ...
Measurement of oxidatively-induced clustered DNA lesions using a novel adaptation of single cell gel electrophoresis (comet assay) [0.03%]
测量氧化诱导的DNA簇损伤的新单细胞凝胶电泳方法
Alexandros G Georgakilas,Stewart M Holt,Jessica M Hair et al.
Alexandros G Georgakilas et al.
The two basic groups of complex DNA damage are double-strand breaks (DSBs) and non-DSB oxidatively-induced clustered DNA lesions (OCDLs). The single-cell gel electrophoresis (SCGE) or comet assay has been widely used for the detection of lo...
Determining membrane protein topologies in single cells and high-throughput screening applications [0.03%]
单细胞和高通量筛选应用中的膜蛋白拓扑结构确定方法
Christian Wunder,Jennifer Lippincott-Schwartz,Holger Lorenz
Christian Wunder
Correct localization and topology are crucial for a protein's cellular function. To determine topologies of membrane proteins, a new technique, called fluorescence protease protection (FPP) assay, has recently been established. The sole req...
Stéphane Belin,Sabine Hacot,Lionel Daudignon et al.
Stéphane Belin et al.
Highly conserved during evolution, the ribosome is the central effector of protein synthesis. In mammalian cells, the ribosome is a macromolecular complex composed of four different ribosomal RNAs (rRNA) and about 80 ribosomal proteins. Req...