Production of Recombinant Transmembrane Proteins from Mammalian Cells for Biochemical and Structural Analyses [0.03%]
用于生化及结构分析的重组人源细胞膜蛋白表达体系
Robbins Puthenveetil,Chul-Jin Lee,Anirban Banerjee
Robbins Puthenveetil
Eukaryotic integral membrane proteins are key components of various biological processes. Because they are implicated in multiple diseases, it is important to understand their mechanism of action by elucidating their structure and function....
Jennifer Martinez
Jennifer Martinez
Phagocytes, notably macrophages, are critical sentinels of their environment, patrolling for and eradicating unwanted components. The ability of cells to process extracellular cargo in an appropriate manner is important for both clearance o...
Generation of 3D Tumor Spheroids with Encapsulating Basement Membranes for Invasion Studies [0.03%]
生成含有基底膜的三维肿瘤球体用于侵袭研究
Shayan S Nazari
Shayan S Nazari
In the past, in vitro studies of invasion and tumor progression were performed primarily using cancer cells cultured on a flat, two-dimensional (2D) surface in a monolayer. In recent years, however, many studies have demonstrated difference...
Optogenetic Control of RhoA to Probe Subcellular Mechanochemical Circuitry [0.03%]
用于探测细胞器机械化学回路的RhoA光遗传学控制
Kate E Cavanaugh,Patrick W Oakes,Margaret L Gardel
Kate E Cavanaugh
Spatiotemporal localization of protein function is essential for physiological processes from subcellular to tissue scales. Genetic and pharmacological approaches have played instrumental roles in isolating molecular components necessary fo...
Isolation of Highly Migratory and Invasive Cells in Three-Dimensional Gels [0.03%]
三维凝胶中高迁移和侵袭性细胞的分离
Takahisa Takino,Takeshi Suzuki,Motoharu Seiki
Takahisa Takino
We developed a modified invasion assay in three-dimensional (3D) gels that permits isolation of invading cells as living cells, termed an invading cell-trapping (iCT) assay. A small cell strainer consisting of nylon mesh with pores of 40-µ...
Vadim Zinchuk,Olga Grossenbacher-Zinchuk
Vadim Zinchuk
The explosive growth of machine learning has provided scientists with insights into data in ways unattainable using prior research techniques. It has allowed the detection of biological features that were previously unrecognized and overloo...
High-Content Screening for Protein-Protein Interaction Modulators Using Proximity Ligation Assay in Primary Neurons [0.03%]
利用原代神经元中邻近连接测定法的高内涵筛选蛋白相互作用调节物
Tiago Mendes,Adrien Herledan,Florence Leroux et al.
Tiago Mendes et al.
The proximity ligation assay (PLA) allows the detection and subcellular localization of protein-protein interactions with high specificity. We recently developed a high-content screening model based on primary hippocampal neurons cultured i...
A Sensitive and Quantitative mKeima Assay for Mitophagy via FACS [0.03%]
基于流式细胞术的线粒体自噬动态检测新方法:mKeima reporter传感器
Chunxin Wang
Chunxin Wang
Mitophagy is a selective autophagy process that specifically removes damaged mitochondria via general autophagy. The two major recessive Parkinson's disease genes PINK1 and Parkin play essential roles in mitophagy initiation, increasing the...
In Situ Visualization of Telomere Length, Telomere Elongation, and TERT Expression in Single Cells [0.03%]
单细胞端粒长度、端粒延伸和TERT表达的原位可视化
Ajay Ravindranathan,Morgan E Diolaiti,Beth A Cimini et al.
Ajay Ravindranathan et al.
Telomerase plays a critical role in cancer and aging by adding hexa-nucleotide repeats to the ends of telomeres and extending the cellular proliferative lifespan. The very low level of telomerase expression in most cell populations and the ...
Preparation and Co-Culture of iPSC-Derived Dopaminergic Neurons and Astrocytes [0.03%]
诱导多能干细胞来源的多巴胺神经元和星形胶质细胞的培养及共培养
Aurelie de Rus Jacquet
Aurelie de Rus Jacquet
Induced pluripotent stem cell (iPSC)-based models are powerful tools to study neurodegenerative diseases such as Parkinson's disease. The differentiation of patient-derived neurons and astrocytes allows investigation of the molecular mechan...