A Paul Mould
A Paul Mould
In this unit, methods for the analysis of integrin-dependent adhesion are described. Two major types of assays are commonly used for this analysis. The first are cell adhesion assays (as described in UNIT 9.1). A key application of this typ...
Methods for growing and titrating African swine fever virus: field and laboratory samples [0.03%]
非洲猪瘟病毒的培养和滴度测定方法：现场和实验室样品
Angel L Carrascosa,M Jose Bustos,Patricia de Leon
Angel L Carrascosa
Growing African swine fever virus (ASFV) isolates obtained mainly from the field, but also engineered in the laboratory, is a critical step for diagnosis, titration, or virus infection studies. This unit describes a set of methods and proto...
Interaction trap/two-hybrid system to identify interacting proteins [0.03%]
用于识别互作蛋白的interaction trap/two-hybrid系统
Erica A Golemis,Ilya Serebriiskii,Russell L Finley Jr. (hunt by interaction mating) et al.
Erica A Golemis et al.
The yeast two-hybrid method (or interaction trap) is a powerful technique for detecting protein interactions. The procedure is performed using transcriptional activation of a dual reporter system in yeast to identify interactions between a ...
Allosteric activation of kinases: design and application of RapR kinases [0.03%]
变构激活激酶的作用及应用：雷帕霉素调节激酶的设计与应用研究
Andrei V Karginov,Klaus M Hahn
Andrei V Karginov
Here we describe a method for the engineered regulation of protein kinases in living cells, the design and application of RapR (rapamycin regulated) kinases. The RapR kinase method enables activation of kinases with high specificity and pre...
Sean Gallagher,Scott E Winston,Steven A Fuller et al.
Sean Gallagher et al.
Immunoblotting (western blotting) is used to identify specific antigens recognized by polyclonal or monoclonal antibodies. This unit provides protocols for all steps, starting with solubilization of the protein samples, usually by means of ...
Quantitative colocalization analysis of confocal fluorescence microscopy images [0.03%]
共焦荧光显微图像的定量同域分析方法研究
Vadim Zinchuk,Olga Grossenbacher-Zinchuk
Vadim Zinchuk
Colocalization is an important finding in many cell biological studies. This unit describes a protocol for quantitative evaluation of images with colocalization based on the calculation of a number of specialized coefficients. First, images...
A dual-chamber model of the female genital tract to evaluate epithelial toxicity of candidate anti-HIV microbicides [0.03%]
一个评估抗艾滋病毒候选药物的上生殖道双室模型
Kevin K Ariën,Guido Vanham,Youssef Gali
Kevin K Ariën
Heterosexual transmission of human immunodeficiency virus (HIV) is the predominant mode of infection worldwide. The early steps of transepithelial infection are crucial, but how exactly infection is established in the female genital tract (...
Huntington Potter,Richard Heller
Huntington Potter
Electroporation--the use of high-voltage electric shocks to introduce DNA into cells--can be used with most cell types, yields a high frequency of both stable transformation and transient gene expression, and, because it requires fewer step...
Randall I Krohn
Randall I Krohn
Protein quantification is an important step for handling protein samples for isolation and characterization; it is a prerequisite step before submitting proteins for chromatographic, electrophoretic, or immunochemical analysis and separatio...
Donna M Tscherne,Adolfo García-Sastre
Donna M Tscherne
This unit describes a viral entry assay where a beta-lactamase reporter protein fused to the matrix protein of either influenza (BlaM1) or ebola virus (BlaVP40) is packaged as a structural component into virus-like particles (VLPs). The Bla...