Cardiac differentiation of human embryonic stem cells and their assembly into engineered heart muscle [0.03%]
人胚胎干细胞的心脏分化及工程化心肌的构建
Poh Loong Soong,Malte Tiburcy,Wolfram-Hubertus Zimmermann
Poh Loong Soong
The advent of pluripotent human embryonic stem cells has created the unique opportunity for the development of a wide variety of humanized cellular tools for basic research, as well as industrial and clinical applications. It has, however, ...
Approaches to studying Arf GAPs in cells: in vitro assay with isolated focal adhesions [0.03%]
细胞中Arf_GAP的研究方法——与分离焦粘着斑的体外试验
Pei-Wen Chen,Xiaoying Jian,Ruibai Luo et al.
Pei-Wen Chen et al.
The Arf GAPs are a family of proteins with a common catalytic function of hydrolyzing GTP bound to ADP-ribosylation factors (Arf) with proposed cellular functions that are diverse (Inoue and Randazzo, 2007; Kahn et al., 2008). Understanding...
Metabolic labeling with noncanonical amino acids and visualization by chemoselective fluorescent tagging [0.03%]
非编码氨基酸的代谢标记及化学选择性荧光标记可视化技术
Susanne Tom Dieck,Anke Müller,Anne Nehring et al.
Susanne Tom Dieck et al.
Fluorescent labeling of proteins by genetically encoded fluorescent protein tags has enabled an enhanced understanding of cell biological processes but is restricted to the analysis of a limited number of identified proteins. This approach ...
Philipp Isermann,Patricia M Davidson,Josiah D Sliz et al.
Philipp Isermann et al.
The nucleus is the characteristic hallmark of all eukaryotic cells. The physical properties of the nucleus reflect important biological characteristics, such as chromatin organization or nuclear envelope composition; they can also directly ...
Analysis of interactions between genomic loci through Chromosome Conformation Capture (3C) [0.03%]
染色体构象捕获(3C)技术分析基因组位点间的相互作用
Belal El Kaderi,Scott Medler,Athar Ansari
Belal El Kaderi
Genome architecture plays a significant role in the regulation of DNA-based cellular processes such as transcription and recombination. The successful accomplishment of these processes involves coordinated interaction of DNA elements locate...
Yukinori Endo
Yukinori Endo
Important events in embryonic development such as gastrulation, neurulation, and cranial neural crest development occur in ectodermal tissues during vertebrate embryonic development. Although the chicken embryo is a well-established model s...
Pamela Nicholson,Raphael Joncourt,Oliver Mühlemann
Pamela Nicholson
The nonsense-mediated mRNA decay (NMD) pathway acts to selectively identify and degrade mRNAs that contain a premature translation termination codon (PTC), and hence reduce the accumulation of potentially toxic truncated proteins. NMD is on...
Analysis of nonsense-mediated mRNA decay in Saccharomyces cerevisiae [0.03%]
模式酵母Saccharomyces cerevisiae中无意义介导的mRNA降解分析
Bessie W Kebaara,Kristian E Baker,Krista D Patefield et al.
Bessie W Kebaara et al.
Nonsense-mediated mRNA decay is a highly conserved pathway that degrades mRNAs with premature termination codons. These mRNAs include mRNAs transcribed from nonsense or frameshift alleles as well as wild-type mRNA with signals that direct r...
Analysis of polarized membrane traffic in hepatocytes and hepatic cell lines [0.03%]
肝细胞和肝细胞系中极化膜运输的分析研究
Julie G In,Gudrun Ihrke,Pamela L Tuma
Julie G In
The protocols described in this unit were developed to monitor membrane traffic in cultured cell monolayers that display hepatic polarity. In general, the assays are designed to visualize and/or quantitate membrane trafficking by monitoring...
Fiona M Menzies,Kevin Moreau,Claudia Puri et al.
Fiona M Menzies et al.
Macroautophagy (referred to herein as autophagy) is a process in which cells engulf portions of cytoplasm in double-membrane vesicles called autophagosomes. These autophagosomes can also capture protein oligomers associated with neurodegene...