Katy Phelan,Kristin M May
Katy Phelan
Cultured mammalian cells are used extensively in cell biology studies. It requires a number of special skills in order to be able to preserve the structure, function, behavior, and biology of the cells in culture. This unit describes the ba...
Freeze-fracture immunocytochemistry: fracture-label and label-fracture for the localization of membrane proteins [0.03%]
冷冻断裂免疫细胞化学定位膜蛋白的裂隙标记和标记裂隙方法研究
Frederick W K Kan
Frederick W K Kan
Freeze-fracture is a unique investigative tool for visualization of the en face topography of individual membrane leaflets of cell membranes at high resolution under the electron microscope. The development of a system of freeze-fracture cy...
Haiqing Fu,Emilie Besnard,Romain Desprat et al.
Haiqing Fu et al.
Recent advances in genome-sequencing technology have led to the complete mapping of DNA replication initiation sites in the human genome. This thorough origin mapping facilitates understanding of the relationship between replication initiat...
Analysis of Copy-Number Alterations in Single Cells Using Microarray-Based Comparative Genomic Hybridization (aCGH) [0.03%]
基于微阵列 comparative genomic hybridization(aCGH)分析单细胞中的拷贝数变异
Birte Möhlendick,Nikolas H Stoecklein
Birte Möhlendick
In this unit, we describe a workflow that enables array comparative genomic hybridization (aCGH) of single cells. The unit first describes the isolation and preparation of single peripheral mononuclear cells from blood (PBMC) to prepare a s...
Standardized isolation and culture of murine liver sinusoidal endothelial cells [0.03%]
小鼠肝窦内皮细胞的分离培养及鉴定
Rajkumar Cheluvappa
Rajkumar Cheluvappa
Isolation of murine liver sinusoidal endothelial cells (LSECs) is an exacting and finicky procedure. After exhaustive standardization, we were able to devise an easily reproducible protocol which produced consistent results. Moreover, we sc...
A High-Content Assay for Biosensor Validation and for Examining Stimuli that Affect Biosensor Activity [0.03%]
用于生物传感器验证及检测影响生物传感器活性刺激因素的高内涵筛选模型
Scott D Slattery,Klaus M Hahn
Scott D Slattery
Biosensors are valuable tools used to monitor many different protein behaviors in vivo. Demand for new biosensors is high, but their development and characterization can be difficult. During biosensor design, it is necessary to evaluate the...
Daiji Kiyozumi,Ryoko Sato-Nishiuchi,Kiyotoshi Sekiguchi
Daiji Kiyozumi
Integrins are cell surface receptors for cell adhesion. Integrin-mediated cell adhesion regulates various cellular processes, including cell survival, migration, proliferation, and differentiation. In vivo, ligands for integrins are immobil...
Thomas V Ramos,Aby J Mathew,Maria L Thompson et al.
Thomas V Ramos et al.
Cryopreservation is the use of low temperatures to preserve structurally intact living cells. The cells that survive the thermodynamic journey from the 37 °C incubator to the -196 °C liquid nitrogen storage tank are free from the influenc...
Emilie Besnard,Romain Desprat,Michael Ryan et al.
Emilie Besnard et al.
Understanding the regulatory principles ensuring complete DNA replication in each cell division is critical for deciphering the mechanisms that maintain genomic stability. Recent advances in genome sequencing technology facilitated complete...
Rapid Depletion of Budding Yeast Proteins via the Fusion of an Auxin-Inducible Degron (AID) [0.03%]
通过融合 auxin 诱导型降解域(AID) 快速耗竭芽殖酵母蛋白质
Kohei Nishimura,Masato T Kanemaki
Kohei Nishimura
The auxin-inducible degron (AID) system allows the rapid and reversible proteolysis of proteins of interest, and enables the generation of conditional mutants of budding yeast. The construction of budding yeast AID mutants is simple, and th...