Yanjie Li,Robert J Tomko Jr,Mark Hochstrasser
Yanjie Li
In eukaryotes, damaged or unneeded proteins are typically degraded by the ubiquitin-proteasome system. In this system, the protein substrate is often first covalently modified with a chain of ubiquitin polypeptides. This chain serves as a s...
Sang-Soo Kim,Kathleen F Pirollo,Esther H Chang
Sang-Soo Kim
In many human cancers including malignant glioblastoma multiforme (GBM), cancer stem cells (CSCs) are thought to be responsible for tumor initiation, metastasis and resistance to conventional anti-cancer therapies. Therefore, a CSC-targeted...
Visualizing G Protein-Coupled Receptor-Receptor Interactions in Brain Using Proximity Ligation In Situ Assay [0.03%]
使用原位邻近标记技术可视化大脑中的G蛋白偶联受体-受体相互作用
Jaume Taura,Víctor Fernández-Dueñas,Francisco Ciruela
Jaume Taura
G protein-coupled receptors (GPCRs) constitute the largest family of plasma membrane receptors, thus representing the more investigated drug targets in the design of new therapeutic strategies. The existence of receptor-receptor interaction...
Identification and Characterization of Tunneling Nanotubes for Intercellular Trafficking [0.03%]
用于细胞间运输的穿隧纳米管的识别与特征分析
Saïda Abounit,Elise Delage,Chiara Zurzolo
Saïda Abounit
Tunneling nanotubes (TNTs) are thin membranous channels providing direct cytoplasmic connection between remote cells. They are commonly observed in different cell cultures and increasing evidence supports their role in intercellular communi...
In Vitro Analysis of the Very-Low Density Lipoprotein Export from the Trans-Golgi Network [0.03%]
极低密度脂蛋白从反面高尔基体网络分泌的体外分析
Shadab A Siddiqi
Shadab A Siddiqi
The movement of mature VLDL particles from the TGN to the plasma membrane (PM) is a complex physiological process that plays a critical role in hepatic lipid homeostasis. However, the molecular mechanisms regulating these intracellular tran...
Daiji Kiyozumi,Ryoko Sato-Nishiuchi,Kiyotoshi Sekiguchi
Daiji Kiyozumi
Integrins are cell surface receptors for cell adhesion. Integrin-mediated cell adhesion regulates various cellular processes, including cell survival, migration, proliferation, and differentiation. In vivo, ligands for integrins are immobil...
Matthew C Jones,Jonathan D Humphries,Adam Byron et al.
Matthew C Jones et al.
The integration of cells with their extracellular environment is facilitated by cell surface adhesion receptors, such as integrins, which play important roles in both normal development and the onset of pathologies. Engagement of integrins ...
Reconstitution of a bioengineered salivary gland using a three-dimensional cell manipulation method [0.03%]
利用三维细胞操作方法再现生物工程化唾液腺结构
Miho Ogawa,Takashi Tsuji
Miho Ogawa
One concept in regenerative therapy is the replacement of a lost or damaged organ with a regenerated, fully functional organ. Three-dimensional cell manipulation techniques, designated "organ germ methods," enable the normal development of ...
Adhesive micropatterns to study intermediate filament function in nuclear positioning [0.03%]
用于研究细胞核定位的中间丝功能的粘附微图案
Isabelle Dupin,Julien Elric,Sandrine Etienne-Manneville
Isabelle Dupin
The nucleus is generally found near the cell center; however its position can vary in response to extracellular or intracellular signals, leading to a polarized intracellular organization. Nuclear movement is mediated by the cytoskeleton an...
Alice Klausmeyer,D Stern,S Wiese
Alice Klausmeyer
Isolated spinal motoneurons are a powerful tool for studying basic mechanisms of neurite growth and survival. Since motoneurons are a minor population of developing spinal cord cells, they need to be purified and enriched to separate them f...