Super-Resolution Microscopy and Single-Protein Tracking in Live Bacteria Using a Genetically Encoded, Photostable Fluoromodule [0.03%]
用于活细菌的超分辨率显微镜和单蛋白追踪的遗传编码、光稳定性荧光模块
Saumya Saurabh,Adam M Perez,Colin J Comerci et al.
Saumya Saurabh et al.
Visualization of dynamic protein structures in live cells is crucial for understanding the mechanisms governing biological processes. Fluorescence microscopy is a sensitive tool for this purpose. In order to image proteins in live bacteria ...
Microcontact Peeling: A Cell Micropatterning Technique for Circumventing Direct Adsorption of Proteins to Hydrophobic PDMS [0.03%]
微接触移除法：一种用于避免蛋白质直接吸附到疏水型PDMS上的细胞微图案技术
Sho Yokoyama,Tsubasa S Matsui,Shinji Deguchi
Sho Yokoyama
Microcontact printing (μCPr) is one of the most popular techniques used for cell micropatterning. In conventional μCPr, a polydimethylsiloxane (PDMS) stamp with microfeatures is used to adsorb extracellular matrix (ECM) proteins onto the ...
Labeling DNA Replication Foci to Visualize Chromosome Territories In Vivo [0.03%]
标记DNA复制焦点以在体内可视化染色体区域
Apolinar Maya-Mendoza,Dean A Jackson
Apolinar Maya-Mendoza
While a detailed understanding of chromatin dynamics is needed to explain how higher-order chromatin organization influences nuclear function, the molecular principles that regulate chromatin mobility in mammalian nuclei remain largely unkn...
Labeling and Magnetic Resonance Imaging of Exosomes Isolated from Adipose Stem Cells [0.03%]
脂肪干细胞外泌体的标记及磁共振成像研究
Alice Busato,Roberta Bonafede,Pietro Bontempi et al.
Alice Busato et al.
Adipose stem cells (ASC) represent a promising therapeutic approach for neurodegenerative diseases. Most biological effects of ASC are probably mediated by extracellular vesicles, such as exosomes, which influence the surrounding cells. Cur...
Traction Force Microscopy in 3-Dimensional Extracellular Matrix Networks [0.03%]
三维细胞外基质网络中的牵引力显微镜技术
M Cóndor,J Steinwachs,C Mark et al.
M Cóndor et al.
Cell migration through a three-dimensional (3-D) matrix depends strongly on the ability of cells to generate traction forces. To overcome the steric hindrance of the matrix, cells need to generate sufficiently high traction forces but also ...
Patterning on Topography for Generation of Cell Culture Substrates with Independent Nanoscale Control of Chemical and Topographical Extracellular Matrix Cues [0.03%]
利用拓扑结构进行图案化以生成具有独立纳米级调控的化学和拓扑细胞培养基底
Emily N Sevcik,John M Szymanski,Quentin Jallerat et al.
Emily N Sevcik et al.
The cell microenvironment plays an important role in many biological processes, including development and disease progression. Key to this is the extracellular matrix (ECM), a complex biopolymer network serving as the primary insoluble sign...
Duojiao Ni,Peng Xu,Sean Gallagher
Duojiao Ni
Immunoblotting (western blotting) is used to identify specific antigens recognized by polyclonal or monoclonal antibodies. This unit provides protocols for all steps, starting with solubilization of the protein samples, usually by means of ...
Discovering Protein-Protein Interactions Using Nucleic Acid Programmable Protein Arrays [0.03%]
利用核酸编程蛋白质阵列发现蛋白质-蛋白质相互作用
Yanyang Tang,Ji Qiu,Matthias Machner et al.
Yanyang Tang et al.
We have developed a protocol enabling the study of protein-protein interactions (PPIs) at the proteome level using in vitro-synthesized proteins. Assay preparation requires molecular cloning of the query gene into a vector that supports in ...
Ivan T Rebustini
Ivan T Rebustini
The increasing repertoire of microRNAs expressed during organ development and their role in regulating organ morphogenesis provide a compelling need to develop methods to assess microRNA function using various in vitro and in vivo experimen...
A Unique High-Throughput Assay to Identify Novel Small Molecule Inhibitors of Chemotaxis and Migration [0.03%]
一种新颖的高通量筛选体系用于寻找化学趋化和迁移的小分子抑制剂
Xin-Hua Liao,Alan R Kimmel
Xin-Hua Liao
Chemotaxis and cell migration play pivotal roles in normal physiological processes such as embryogenesis, inflammation, and wound healing, as well as in pathological processes including chronic inflammatory disease and cancer metastasis. No...