PseudoBase: a genomic visualization and exploration resource for the Drosophila pseudoobscura subgroup [0.03%]
伪果蝇基因组可视化和探索资源
Katharine L Korunes,Russell B Myers,Ryan Hardy et al.
Katharine L Korunes et al.
Drosophila pseudoobscura is a classic model system for the study of evolutionary genetics and genomics. Given this long-standing interest, many genome sequences have accumulated for D. pseudoobscura and closely related species D. persimilis...
Exploring Excitotoxicity and Regulation of a Constitutively Active TRP Ca2+ Channel in Drosophila [0.03%]
探索TRP钙离子通道兴奋毒性和调节作用——果蝇中的研究
Bih-Hwa Shieh,Lucinda Nuzum,Inga Kristaponyte
Bih-Hwa Shieh
Unregulated Ca2+ influx affects intracellular Ca2+ homoeostasis, which may lead to neuronal death. In Drosophila, following the activation of rhodopsin the TRP Ca2+ channel is open to mediate the light-dependent depolarization. A constituti...
Deena Damschroder,Kristin Richardson,Tyler Cobb et al.
Deena Damschroder et al.
The use of the Drosophila model for studying the broad beneficial effects of exercise training has grown over the past decade. As work using Drosophila as an exercise model becomes more widespread, the influence of genetic background on per...
Short is still sweet [0.03%]
短小依然精悍
Howy Jacobs
Howy Jacobs
Ruijian Guo,Anna-Lena Henke,Klaus Reinhardt
Ruijian Guo
Sperm quality, an important male fitness trait, is commonly compared between studies. However, few studies consider how genetic and environmental variation affect sperm quality, even in the genetic model Drosophila melanogaster. Here we sho...
A pipeline for precise and efficient genome editing by sgRNA-Cas9 RNPs in Drosophila [0.03%]
通过sgRNA-Cas9核糖核蛋白在果蝇中实现精确高效基因组编辑的流程
Kevin G Nyberg,Joseph Q Nguyen,Yong-Jae Kwon et al.
Kevin G Nyberg et al.
Genome editing via homology-directed repair (HDR) has made possible precise and deliberate modifications to gene sequences. CRISPR/Cas9-mediated HDR is the simplest means to carry this out. However, technical challenges remain to improve ef...
Sushmitha Hegde,Amarendranath Soory,Bhagyashree Kaduskar et al.
Sushmitha Hegde et al.
Post-translational modifications (PTMs) are critical drivers and attenuators for proteins that regulate immune signalling cascades in host defence. In this review, we explore functional roles for one such PTM, the small ubiquitin-like modif...
Drosophila GFAT1 and GFAT2 enzymes encode obligate developmental functions [0.03%]
果蝇GFAT1和GFAT2酶编码必要的发育功能
Po Chen,Sarah Visokay,John M Abrams
Po Chen
Glutamine: fructose-6-phosphate amidotransferase (GFAT) enzymes catalyse the first committed step of the hexosamine biosynthesis pathway (HBP) using glutamine and fructose-6-phosphate to form glucosamine-6-phosphate (GlcN6P). Numerous speci...
A method for the permeabilization of live Drosophila melanogaster larvae to small molecules and cryoprotectants [0.03%]
一种使活的果蝇幼虫渗透小分子和低温保护剂的方法
Alex Murray,Daniel Palmer,Daimark Bennett et al.
Alex Murray et al.
The larvae of Drosophila melanogaster is a model organism widely used to study the muscular and nervous systems. Drosophila larvae are surrounded by a waxy cuticle that prevents permeation by most substances. Here we develop a method to rem...
Steven J Marygold,Helen Attrill,Elena Speretta et al.
Steven J Marygold et al.
DNA synthesis during replication or repair is a fundamental cellular process that is catalyzed by a set of evolutionary conserved polymerases. Despite a large body of research, the DNA polymerases of Drosophila melanogaster have not yet bee...