Gateway cloning vectors for the LexA-based binary expression system in Drosophila [0.03%]
果蝇中基于LexA的二元表达体系的载体_gateway克隆载体
Soeren Diegelmann,Michael Bate,Matthias Landgraf
Soeren Diegelmann
Studies on the formation of connections in the developing nervous system are greatly aided by methods that permit the differential visualisation and manipulation of pre- and postsynaptic partner neurons. This has been facilitated by the adv...
Sick of mating: sexual transmission of a pathogenic bacterium in Drosophila melanogaster [0.03%]
厌倦了交配：果蝇中性传播病原菌的现象
Tanner S Miest,Margaret Bloch-Qazi
Tanner S Miest
Internal fertilization protects gametes from inhospitable environments and ensures sufficient proximity for gamete union. However, close contact between individuals during mating also increases the risk of pathogen transfer. We developed an...
Distinct heparan sulfate compositions in wild-type and pipe-mutant eggshell matrix [0.03%]
野生型和pipe突变型卵壳基质中不同的肝素硫酸盐组成
Youmie Park,Zhenqing Zhang,Robert J Linhardt et al.
Youmie Park et al.
Spatial information embedded in the extracellular matrix establishes the dorsoventral polarity of the Drosophila embryo through the ventral activity of a serine protease cascade. Pipe is a Golgi-localized protein responsible for generating ...
Changes in cuticular lipids, water loss and desiccation resistance in a tropical drosophilid: analysis of variation between and within populations [0.03%]
一种热带果蝇表皮脂质、失水率及抗干旱能力的变化分析：种群间和种群内的变异
Ravi Parkash,Bhawna Kalra,Vineeta Sharma
Ravi Parkash
We investigated within as well as between population variability in desiccation resistance, cuticular lipid mass per fly and cuticular water loss in nine geographical populations of a tropical drosophilid, Zaprionus indianus. Interestingly,...
Comparative Study
Fly. 2008 Jul-Aug;2(4):189-97. DOI:10.4161/fly.6619 2008
Tandem affinity purification in Drosophila: the advantages of the GS-TAP system [0.03%]
果蝇串联亲和纯化技术：GS-TAP系统的优势
Phillip Kyriakakis,Marla Tipping,Louka Abed et al.
Phillip Kyriakakis et al.
Tandem affinity purification (TAP) has been widely used for the analysis of protein complexes. We investigated the parameters of the recently developed TAP method (GS-TAP) and its application in Drosophila. This new tag combination includes...
Cloning of the neurodegeneration gene drop-dead and characterization of additional phenotypes of its mutation [0.03%]
死亡基因drop-dead克隆及该基因突变的其他表型分析
Edward M Blumenthal
Edward M Blumenthal
Mutations in the Drosophila gene drop-dead (drd) result in early adult lethality and neurodegeneration, but the molecular identity of the drd gene and its mechanism of action are not known. This paper describes the characterization of a new...
The Drosophila protein palmitoylome: characterizing palmitoyl-thioesterases and DHHC palmitoyl-transferases [0.03%]
果蝇棕榈酰化蛋白质组：鉴定棕榈酰基硫酯酶和DHHC棕榈酰转移酶
Barbra A Bannan,Jamie Van Etten,John A Kohler et al.
Barbra A Bannan et al.
Palmitoylation is the post-translational addition of a palmitate moiety to a cysteine residue through a covalent thioester bond. The addition and removal of this modification is controlled by both palmitoyl acyl-transferases and thioesteras...
Matter arising: off-targets and genome-scale RNAi screens in Drosophila [0.03%]
fly中脱靶效应和基于RNA干扰的全基因组筛选中的新问题
Norbert Perrimon,Bernard Mathey-Prevot
Norbert Perrimon
Recently, the issue of off-target effects (OTEs) associated with long double stranded RNAs (dsRNAs) used in RNAi screens, such as those performed at the Drosophila RNAi Screening Center and other laboratories, has become a focus of great in...
Roles for Drp1, a dynamin-related protein, and milton, a kinesin-associated protein, in mitochondrial segregation, unfurling and elongation during Drosophila spermatogenesis [0.03%]
Drp1（一种与dynamin相关的蛋白）和milton（一种与kinesin结合的蛋白）在果蝇精子发生过程中线粒体分隔、伸展和延长中的作用
Amanda C Aldridge,Levi P Benson,Monica M Siegenthaler et al.
Amanda C Aldridge et al.
Mitochondria undergo dramatic rearrangement during Drosophila spermatogenesis. In wild type testes, the many small mitochondria present in pre-meiotic spermatocytes later aggregate, fuse, and interwrap in post-meiotic haploid spermatids to ...
Drosophila as a model for human diseases: IRB and ICREA, Barcelona, Spain, October 5-7, 2006 [0.03%]
果蝇作为人类疾病的研究模型：西班牙巴塞罗那大学IRB和ICREA，2006年10月5-7日研讨会
Nikolaos A Tountas,Mark E Fortini
Nikolaos A Tountas