Flow cytometry and karyotype analysis of D.melanogaster eye disc cells [0.03%]
模式生物Drosophila眼碟细胞的流式细胞分析及染色体核型分析研究
Marianna Collesano,David F V Corona
Marianna Collesano
The developing Drosophila eye-antennal disc is a particularly suited system for the genetic and cellular studies of complex biological processes. Methods to analyze Drosophila eye discs by flow cytometry are mainly based on the dissociation...
Kwang-Wook Choi,Sang-Chul Nam,Bibhash Mukhopadhyay
Kwang-Wook Choi
Apical basal cell polarity is a fundamental feature of all epithelial cells. Identification of the genes involved in the polarization of epithelial cells has begun to reveal the mechanisms underlying the establishment and maintenance of cel...
Yu-ting Chou,Bergin Tam,Fabien Linay et al.
Yu-ting Chou et al.
RNA silencing functions as an adaptive antiviral defense in both plants and animals. In turn, viruses commonly encode suppressors of RNA silencing, which enable them to mount productive infection. These inhibitor proteins may be exploited a...
Xin Chen
Xin Chen
Studies in recent years have resulted in rapid progress in the reprogramming of terminally differentiated somatic cells toward their embryonic and pluripotent states. These discoveries not only allowed the understanding of the unique "stemn...
A green fluorescent protein-based reporter for protein nuclear import studies in Drosophila cells [0.03%]
基于绿色荧光蛋白的蛋白质核输入研究报告基因应用于果蝇细胞中
Wing Man Chan,Pang Chui Shaw,Ho Yin Edwin Chan
Wing Man Chan
Green fluorescent protein-based reporters are commonly used to investigate protein nucleocytoplasmic transport. In this study we developed a novel reporter GFP2-GST which consists of two copies of GFP and one copy of GST, and tested it in t...
Drosophila polymorphism database (DPDB): a portal for nucleotide polymorphism in Drosophila [0.03%]
果蝇多态性数据库(DPDB)：果蝇核苷酸多态性的门户网站
Sònia Casillas,Raquel Egea,Natalia Petit et al.
Sònia Casillas et al.
As a growing number of haplotypic sequences from resequencing studies are now accumulating for Drosophila in the main primary sequence databases, collectively they can now be used to describe the general pattern of nucleotide variation acro...
Darby J Carlson,Anjeza Pashaj,Kylee Gardner et al.
Darby J Carlson et al.
Drosophila melanogaster is an ideal model organism for various types of aging studies. They are easy to maintain, relatively inexpensive, have short life cycles, provide large sample sizes, and can be genetically manipulated via various met...
Gerard Campbell,Stuart J Newfeld
Gerard Campbell
At the 49(th) Annual Drosophila Research Conference from April 3-8, 2008 in San Diego there were eight talks and over ninety posters in the section on Organogenesis and Gametogenesis. These covered a wide range of topics within the two broa...
reduced ocelli encodes the leucine rich repeat protein Pray For Elves in Drosophila melanogaster [0.03%]
减少的复眼在果蝇中编码富含亮氨酸的重复蛋白“为精灵祈祷”
Jason C Caldwell,Sarah K Fineberg,Daniel F Eberl
Jason C Caldwell
The ocelli are three simple photoreceptors on the vertex of the fruit fly head. We sought to identify the gene encoded by the classical ocellar mutant, reduced ocelli (rdo). Deficiency and inversion breakpoint mapping and P-element induced ...
Andrea Barrett,Stephanie Krueger,Megan Jackson et al.
Andrea Barrett et al.
Transgenes, especially those driving production of the GAL4 transcription factor in a specific spatial pattern, are a critical and widely used tool in the Drosophila research community. We recently noticed loss of GAL4-driven reporter gene ...