Tools and strategies for scarless allele replacement in Drosophila using CRISPR/Cas9 [0.03%]
在果蝇中使用CRISPR / Cas9进行无疤痕等位基因置换的工具和策略
Abigail M Lamb,Elizabeth A Walker,Patricia J Wittkopp
Abigail M Lamb
Genome editing via the CRISPR/Cas9 RNA-guided nuclease system has opened up exciting possibilities for genetic analysis. However, technical challenges associated with homology-directed repair have proven to be roadblocks for producing chang...
Marta Morey
Marta Morey
Neurons form precise patterns of connections. The cellular recognition mechanisms regulating the selection of synaptic partners are poorly understood. As final mediators of cell-cell interactions, cell surface and secreted molecules (CSMs) ...
Revisiting the role of the Gcm transcription factor, from master regulator to Swiss army knife [0.03%]
重新审视Gcm转录因子的作用：从万能调节因子到多功能工具分子
Pierre B Cattenoz,Angela Giangrande
Pierre B Cattenoz
Master genes are known to induce the differentiation of a multipotent cell into a specific cell type. These molecules are often transcription factors that switch on the regulatory cascade that triggers cell specification. Gcm was first desc...
Deborah A Hursh,Brian G Stultz,Sung Yeon Park
Deborah A Hursh
decapentaplegic (dpp), the Drosophila ortholog of BMP 2/4, directs ventral adult head morphogenesis through expression in the peripodial epithelium of the eye-antennal disc. This dpp expressing domain exerts effects both on the peripodial e...
Marcus Michel,Christian Dahmann
Marcus Michel
During animal development, cells with similar function and fate often stay together and sort out from cells with different fates. In Drosophila wing imaginal discs, cells of anterior and posterior fates are separated by a straight compartme...
Infection avoidance behavior: Viral exposure reduces the motivation to forage in female Drosophila melanogaster [0.03%]
感染避讳行为：病毒暴露会降低果蝇的觅食动机
Pedro F Vale,Michael D Jardine
Pedro F Vale
Infection avoidance behaviors are the first line of defense against pathogenic encounters. Behavioral plasticity in response to internal or external cues of infection can therefore generate potentially significant heterogeneity in infection...
WntD and Diedel: Two immunomodulatory cytokines in Drosophila immunity [0.03%]
刺丝泡素和_dieidel_:两种参与果蝇免疫反应的免疫调节细胞因子
Olivier Lamiable,Carine Meignin,Jean-Luc Imler
Olivier Lamiable
Remarkable progress has been made on the understanding of the basic mechanisms of innate immunity in flies, from sensing infection to production of effector molecules. However, how the immune response is orchestrated at the level of the org...
Diaphanous regulates SCAR complex localization during Drosophila myoblast fusion [0.03%]
节肢变态形式调节果蝇肌细胞融合过程中的SCAR复合物定位
Su Deng,Ingo Bothe,Mary Baylies
Su Deng
From Drosophila to man, multinucleated muscle cells form through cell-cell fusion. Using Drosophila as a model system, researchers first identified, and then demonstrated, the importance of actin cytoskeletal rearrangements at the site of f...
Postmating Reproductive isolation between strains of Drosophila willistoni [0.03%]
ドロシフィラ・ウィリス通 strain间的交配后生殖隔离
Xian B Mardiros,Ronni Park,Bryan Clifton et al.
Xian B Mardiros et al.
Speciation can occur through the presence of reproductive isolation barriers that impede mating, restrict cross-fertilization, or render inviable/sterile hybrid progeny. The D. willistoni subgroup is ideally suited for studies of speciation...
Targeted genetics in Drosophila cell lines: Inserting single transgenes in vitro [0.03%]
目标基因遗传学在果蝇细胞系中的应用：体外单转基因的插入
Sathiya N Manivannan,Amanda Simcox
Sathiya N Manivannan
A long-standing problem with analyzing transgene expression in tissue-culture cells is the variation caused by random integration of different copy numbers of transfected transgenes. In mammalian cells, single transgenes can be inserted by ...