A method for the permeabilization of live Drosophila melanogaster larvae to small molecules and cryoprotectants [0.03%]
一种使活的果蝇幼虫渗透小分子和低温保护剂的方法
Alex Murray,Daniel Palmer,Daimark Bennett et al.
Alex Murray et al.
The larvae of Drosophila melanogaster is a model organism widely used to study the muscular and nervous systems. Drosophila larvae are surrounded by a waxy cuticle that prevents permeation by most substances. Here we develop a method to rem...
Steven J Marygold,Helen Attrill,Elena Speretta et al.
Steven J Marygold et al.
DNA synthesis during replication or repair is a fundamental cellular process that is catalyzed by a set of evolutionary conserved polymerases. Despite a large body of research, the DNA polymerases of Drosophila melanogaster have not yet bee...
SILAC-based quantitative proteomic analysis of Drosophila gastrula stage embryos mutant for fibroblast growth factor signalling [0.03%]
基于果蝇FGF信号途径突变体胚胎的定量蛋白质组分析
Hamze Beati,Alistair Langlands,Sara Ten Have et al.
Hamze Beati et al.
Quantitative proteomic analyses in combination with genetics provide powerful tools in developmental cell signalling research. Drosophila melanogaster is one of the most widely used genetic models for studying development and disease. Here ...
Mitochondrial dysfunction generates a growth-restraining signal linked to pyruvate in Drosophila larvae [0.03%]
线粒体功能紊乱通过与乳酸相互作用的方式在果蝇幼虫生长限制信号中产生了一种增长抑制信号
Jack George,Tea Tuomela,Esko Kemppainen et al.
Jack George et al.
The Drosophila bang-sensitive mutant tko25t, manifesting a global deficiency in oxidative phosphorylation due to a mitochondrial protein synthesis defect, exhibits a pronounced delay in larval development. We previously identified a number ...
Expansion of a fly TBI model to four levels of injury severity reveals synergistic effects of repetitive injury for moderate injury conditions [0.03%]
扩展果蝇TBI模型以涵盖四个不同的损伤严重程度并揭示中度损伤条件下重复损伤的协同效应
Lauren J Putnam,Ashley M Willes,Brooke E Kalata et al.
Lauren J Putnam et al.
Several million traumatic brain injury (TBI) events are reported in the United States annually. However, mild TBI events often go unreported, and mild and repetitive mild TBI conditions are challenging to model. Fruit flies (Drosophila mela...
A standardized nomenclature and atlas of the male terminalia of Drosophila melanogaster [0.03%]
果蝇(Drosophila melanogaster)雄性外生殖器的命名法和图谱标准
Gavin Rice,Jean R David,Yoshitaka Kamimura et al.
Gavin Rice et al.
Animal terminalia represent some of the most diverse and rapidly evolving structures in the animal kingdom, and for this reason have been a mainstay in the taxonomic description of species. The terminalia of Drosophila melanogaster, with it...
Bivalency in Drosophila embryos is associated with strong inducibility of Polycomb target genes [0.03%]
双向性与果蝇胚胎中Polycomb靶基因的强诱导性有关
Arslan Akmammedov,Marco Geigges,Renato Paro
Arslan Akmammedov
Polycomb group (PcG) and Trithorax group (TrxG) proteins orchestrate development of a multicellular organism by faithfully maintaining cell fate decisions made early in embryogenesis. An important chromatin mark connected to PcG/TrxG regula...
A modular toolset of phiC31-based fluorescent protein tagging vectors for Drosophila [0.03%]
一系列基于phiC31的荧光蛋白标记载体在果蝇中的应用
Jun Luo,Pingping Shen,Jiong Chen
Jun Luo
The Drosophila transgenic technology and fluorescent protein fusions are powerful tools to analyze protein expression patterns, subcellular localization and protein dynamics. Recently, the Drosophila transgenic technology has been improved ...
Christopher M Groen,Jewel L Podratz,Kevin Treb et al.
Christopher M Groen et al.
Drosophila melanogaster has recently been developed as a simple, in vivo, genetic model of chemotherapy-induced peripheral neuropathy. Flies treated with the chemotherapy agent cisplatin display both a neurodegenerative phenotype and cell d...
Maintenance of Rhodopsin levels in Drosophila photoreceptor and phototransduction requires Protein Kinase D [0.03%]
果蝇视杆细胞中视紫红质的维持需要蛋白激酶D
Sudipta Ashe,Shweta Yadav
Sudipta Ashe
During Drosophila phototransduction, the G protein coupled receptor (GPCR) Rhodopsin (Rh1) transduces photon absorption into electrical signal via G-protein coupled activation of phospholipase C (PLC). Rh1 levels in the plasma membrane are ...