Viral genome editing: striking a balance between promises and precautions [0.03%]
病毒基因组编辑：在承诺与预防之间求平衡
Muhammad Waseem Sajjad,Rubab Zahra Naqvi,Imran Amin
Muhammad Waseem Sajjad
Viral vectors can provide transgene-free genome editing. The forward-looking frameworks for their use must be biosafety and freedom from ecological risk. The responsible innovations, showing a balance between promise and caution following r...
Komal Patel,Samir K Maji
Komal Patel
Amyloid fibrils, known for their stable, cross-β-sheet structure, are involved in both pathological conditions and functional biological processes. While implicated in disease pathogenesis, such as Alzheimer's and Parkinson's, they also pl...
A practical guide to spatial transcriptomics: lessons from over 1000 samples [0.03%]
空间转录组学实用指南：来自逾千样本的启示
Daniela Grases,Eduard Porta-Pardo
Daniela Grases
Spatial transcriptomics (ST) enables the in situ mapping of gene expression, revolutionizing our ability to study tissue organization and cellular interactions. However, many groups struggle with practical barriers to implementation, includ...
Nanobotic microbiomes against emerging vascular pathogens in agriculture [0.03%]
用于农业的纳米机器人微生物群对抗新兴血管病原体
Ayesha Ahmed,Yongmei Li,Yueqiu He et al.
Ayesha Ahmed et al.
Vascular diseases in plants are exceptionally difficult to control. Escalating global climate change further increases the risks of outbreaks from existing and emerging pathogens. Nano-enabled microbiome engineering (NME) represents an inno...
Microbial tools and regulatory innovations for climate-resilient winemaking [0.03%]
微生物工具和管理创新在气候适应性葡萄酒酿造中的应用
Javier Vicente,Santiago Benito,Domingo Marquina et al.
Javier Vicente et al.
Climate-induced acidity loss threatens wine quality, prompting sustainable microbial acidification strategies using Lachancea thermotolerans. This yeast naturally reduces pH and ethanol, aligns with consumer preferences, and benefits from o...
Towards scalable anammox: mechanistic insights and emerging strategies [0.03%]
面向大规模氨氧化耦合厌氧氨氧化技术：机制与策略
Yongguang Li,Yonggang Li,Bing-Jie Ni et al.
Yongguang Li et al.
Anammox-based systems are promising wastewater treatment processes that utilize anaerobic ammonium oxidation (anammox) bacteria to remove nitrogen. These systems offer energy-efficient and carbon-neutral alternatives to conventional biologi...
Kristina Pulejkova,Diethard Mattanovich
Kristina Pulejkova
Semi-automated biofoundry workflows for sequence coevolution-guided isoprene synthase engineering [0.03%]
基于序列协同演化的异戊烯基合成酶工程的半自动化生物工厂流程
Georgii Emelianov,Dong-Uk Song,Aporva Kamath et al.
Georgii Emelianov et al.
Biofoundries serve as transformative platforms for accelerating the engineering of enzymes and microorganisms toward biomanufacturing. In this study, we developed scalable enzyme engineering workflows tailored for biofoundry applications, f...
In vivo continuous evolution via phenotypic sorting to alleviate metabolic bottlenecks in β-alanine production [0.03%]
基于苯丙素分选的体内连续进化缓解β-丙氨酸生产代谢瓶颈研究
Fuqiang Song,Heng Zhang,Ke Wang et al.
Fuqiang Song et al.
β-Alanine is an important platform chemical whose biosynthesis efficiency is limited by pathway bottlenecks and enzyme constraints. In this study, to overcome genetic interactions for β-alanine production, we systematically engineered Esc...
Sustainable protection of multiple hosts against polyphagous pests using Plant Probiotic-Based Gene Silencing [0.03%]
基于植物益生菌的基因沉默可持续保护多种宿主免受杂食性害虫的危害
Jing Chen,Rong Zhang,Xun Zhang et al.
Jing Chen et al.
RNAi is a gene-silencing mechanism mediated by double-stranded RNA (dsRNA) molecules and is a promising pest control technology. However, challenges, such as limited persistence or narrow plant protection spectra in current RNAi application...