Advanced strategies and tools to facilitate and streamline microbial adaptive laboratory evolution [0.03%]
促进和简化微生物适应性实验进化的高级策略和工具
Yinan Wu,Aysha Jameel,Xin-Hui Xing et al.
Yinan Wu et al.
Adaptive laboratory evolution (ALE) has served as a historic microbial engineering method that mimics natural selection to obtain desired microbes. The past decade has witnessed improvements in all aspects of ALE workflow, in terms of growt...
Primate Organoids and Gene-Editing Technologies toward Next-Generation Biomedical Research [0.03%]
灵长类器官和基因编辑技术开启下一代生物医学研究
Chuanxin Chen,Weizhi Ji,Yuyu Niu
Chuanxin Chen
The improved ability to organize pluripotent stem cells (PSCs) into 3D structures in vitro has shed light on organoid technology to recapitulate organs and tumors in vivo. Advances in gene-editing technologies, particularly CRISPR-mediated ...
Hao Gao,Wenming Zhang,Ziyi Yu et al.
Hao Gao et al.
The combination of 3D printing and synthetic biology is a sustainable strategy to fabricate biological objects and systems that behave in a preprogrammed manner. Many microorganisms have been genetically engineered as cell factories for the...
Validation Is Not Verification: Precise Terminology and Scientific Methods in Bioprocess Modeling [0.03%]
验证不等于确认——生物工艺建模的精确术语及科学方法
Jens Smiatek,Alexander Jung,Erich Bluhmki
Jens Smiatek
Advanced statistical approaches and new modeling procedures for biopharmaceutical development and manufacturing have received increasing attention. With this forum article, we would like to highlight the need for a consistent terminology an...
Kelvin C M Lee,Jochen Guck,Keisuke Goda et al.
Kelvin C M Lee et al.
The biophysical properties of cells reflect their identities, underpin their homeostatic state in health, and define the pathogenesis of disease. Recent leapfrogging advances in biophysical cytometry now give access to this information, whi...
Additive Manufacturing Tools to Improve the Performance of Chromatographic Approaches [0.03%]
用于提高色谱法性能的增材制造工具
J F A Valente,F Sousa,N Alves
J F A Valente
Chromatography is widely applied industrially. However, some limitations are associated with its common supports, and the impossibility to fully control their structural features is particularly restrictive. Additive manufacturing (AM) is e...
Leveraging Single-Cell Sequencing for Chimeric Antigen Receptor T Cell Therapies [0.03%]
利用单细胞测序技术促进嵌合抗原受体T细胞治疗的发展
Rocío Castellanos-Rueda,Raphaël B Di Roberto,Fabrice S Schlatter et al.
Rocío Castellanos-Rueda et al.
Chimeric antigen receptor (CAR)-T cell therapies against cancer continue to make inroads in the clinic. However, progress is still hindered by subpar efficacy against many tumors. Gaining a better understanding of CAR-induced T cell activat...
Potency Meets Precision in Nano-optimized Chemotherapeutics [0.03%]
纳米优化化疗药物 potency meets precision
Artavazd Arumov,Asaad Trabolsi,Jonathan H Schatz
Artavazd Arumov
Chemotherapy remains the most widely used cancer treatment modality. Nanotechnology provides exciting opportunities to improve these drugs, transforming decades-old generic treatments into precise new medicines. We illustrate the potential ...
Antoni Gandia,Jeroen G van den Brandhof,Freek V W Appels et al.
Antoni Gandia et al.
Fungi are a revolutionary, smart, and sustainable manufacturing platform that can be used to upcycle byproducts and wastes into flexible fungal materials (FFMs) such as chitin- and β-glucan-based foams, paper, and textiles. With highly ada...
Animal Immunization, in Vitro Display Technologies, and Machine Learning for Antibody Discovery [0.03%]
动物免疫、体外展示技术及机器学习在发现抗体方面的应用
Andreas H Laustsen,Victor Greiff,Aneesh Karatt-Vellatt et al.
Andreas H Laustsen et al.
For years, a discussion has persevered on the benefits and drawbacks of antibody discovery using animal immunization versus in vitro selection from non-animal-derived recombinant repertoires using display technologies. While it has been arg...