Engineering high-quality cultured beef through curcumin-driven metabolic reprogramming in a high-protein scaffold [0.03%]
通过高蛋白支架和姜黄素驱动的代谢重构工程培养高质量的人造牛肉
Dandan Wang,Mei Li,Jun Ma et al.
Dandan Wang et al.
Cultured meat, produced by culturing animal cells in vitro, is emerging as a sustainable alternative to conventional meat. Despite significant progress in production scale-up, inefficient myogenic differentiation remains a key obstacle to a...
Flagellin beyond motility: exploiting flagellar filaments in biotechnology [0.03%]
鞭毛蛋白与运动性之外的生物技术 exploitation of flagellar filaments in biotechnology
Divyangi Pandit,Jacob Scadden,Matthew A B Baker
Divyangi Pandit
Flagellin is the structural protein of the bacterial flagellar filament, an extracellular propeller that rotates to drive swimming in many bacteria. The structure of flagellin allows for outer domain customization, which has been the target...
Self-driving medicine: closed-loop therapeutics for autonomous disease control [0.03%]
自主驾驶药物：用于自动疾病控制的闭环疗法
Gokberk Unal,Martin Fussenegger
Gokberk Unal
Closed-loop gene circuits are paving the way toward self-driving medicine: therapeutics that continuously sense physiological signals, compute responses, and autonomously tune therapeutic output. Yet clinical practice still relies largely o...
Engineered multicomponent bioink enables neuronal maturation and activity in brain-like tissue models [0.03%]
工程化多组分生物墨水可在类脑组织模型中促进神经元成熟和活性
Farnoosh Kalantarnia,Amanda Orr,Somayeh Fardindoost et al.
Farnoosh Kalantarnia et al.
Recreating the physico-mechanical complexities of brain tissue remains a major challenge when engineering neural tissue. Conventional 2D cultures fail to capture the cellular architecture and dynamic environment of the brain while being lab...
Expanding the versatility of the protein master Pichia pastoris towards efficient biosynthesis of diterpenoid sclareol [0.03%]
扩大酿酒毕赤酵母蛋白质.master的多功能性以有效生物合成二萜香薄荷素
Mengyao Zhang,Fan Bai,Yiwei Shen et al.
Mengyao Zhang et al.
Non-conventional yeasts offer unique advantages as tailored cell factories. Pichia pastoris is a master of protein expression and secretion. However, engineering P. pastoris for the overproduction of secondary metabolites is challenging due...
Carbon-efficient upcycling of agrifood waste into fatty acid ester by engineered Clostridium tyrobutyricum [0.03%]
工程化丙酸丁酸梭菌高效利用农牧业废弃物制备脂肪酸酯
Zhihan Yang,Zhenlei Liu,Chao Fang et al.
Zhihan Yang et al.
Microbial conversion of renewable feedstocks into biofuels is gaining interest due to its sustainability. In this research article, we develop a carbon- and cost-efficient fermentation route in Clostridium tyrobutyricum to produce the biofu...
Duckweed biomanufacturing: longstanding promise, decade-long lag, recent renaissance [0.03%]
水生浮游植物的生物制造：长期承诺、十年滞后和近期复兴
Mümin Ibrahim Tek,Sylvain Villot,Dominique Michaud et al.
Mümin Ibrahim Tek et al.
Molecular farming has become a mature, cost-competitive route to recombinant protein, vaccine, and phytochemical production. Nicotiana benthamiana dominates the landscape, while aquatic plants of the family Lemnaceae (duckweeds) continue to...
Julian Rojo-Gallegos,Jason Q Tang,Mark Yarmarkovich et al.
Julian Rojo-Gallegos et al.
Display technologies are a class of experimental methods that enable a direct association between protein function and nucleic acid sequence. Since the introduction of phage display in 1985, antibody display technologies have evolved along ...
CleanFinder: a scalable framework for comprehensive genome editing analysis [0.03%]
高效全面的基因组编辑分析框架CleanFinder
Haribaskar Ramachandran,Jochen Dobner,Thach Nguyen et al.
Haribaskar Ramachandran et al.
Genome editing often generates complex mixtures of alleles rather than single, predefined outcomes. Resolving these heterogeneous edits across diverse editing modalities, sequencing platforms, and multiplexed designs remains a persistent an...
Zi-Xu Zhang,Xiao-Man Sun,He Huang
Zi-Xu Zhang
For microbial functional lipids, industrialization is constrained not by titer alone, but by whether production remains stable during propagation and is scalable in bioreactor operation. Strain stability, dedicated bioreactor design, and fe...