Cheon Woo Moon,Sumin Cho,Minjung Ki et al.
Cheon Woo Moon et al.
Corynebacterium glutamicum is a prominent industrial microorganism traditionally employed for amino acid production, and its utility has been progressively expanded as a chassis for producing diverse valuable chemicals. To fully exploit thi...
Amy Annells,Martin F Breed,Timothy R Cavagnaro et al.
Amy Annells et al.
Targeted acoustic stimulation can modulate fungal growth, metabolism, and molecular activity. It offers a promising route to influence fungal performance in agriculture, biotechnology, ecosystem restoration, and medicine. In this opinion ar...
Automated, modular assembly of reconstituted cell-free systems from in vitro-produced components [0.03%]
用于重构无细胞系统模块化自动装配的体外产生组件
Byeongmin Chae,Hyeongwoo Park,Hyewon Jeon et al.
Byeongmin Chae et al.
High-performance cell-free protein synthesis has transformative potential for synthetic biology, yet the prohibitive costs of Protein synthesis Using Recombinant Elements kits and the labor intensity of in-house preparation have restricted ...
Omics-powered environmental safety assessment framework for gene-edited livestock [0.03%]
基于组学的基因编辑家畜环境安全评价框架
Taotao Gu,Wei Fu,Kui Li et al.
Taotao Gu et al.
Gene editing (GE) pushes livestock breeding toward greater productivity and sustainability. Omics can help assess the environmental safety of gene-edited animals but comes with limitations. In this article, we propose a tiered framework for...
Living buildings with living electronics: towards biologically intelligent biohybrids [0.03%]
带有活电子的活建筑：走向生物智能生物混合体
Pablo Carbonell,Rachel Armstrong,Jorge Barriuso et al.
Pablo Carbonell et al.
Harnessing the innate growth, self-repair, and adaptive capabilities of living systems within engineered devices could transform static buildings via domestic infrastructures into dynamic, self-sustaining platforms. Electroactive biofilms (...
Hollow-fibre biomanufacturing and cell-free engineering of HEK293 extracellular vesicles [0.03%]
中空纤维生物制造和无细胞工程HEK293细胞外囊泡
Richard J R Kelwick,Alexander J Webb,Amelie Heliot et al.
Richard J R Kelwick et al.
Extracellular vesicles (EVs) are lipid-delimited particles produced by most cell types that can have therapeutic effects. Cell-free gene expression systems can be used to produce membrane proteins in vitro, which can integrate with exogenou...
Daojing Wang
Daojing Wang
Capillary electrophoresis-mass spectrometry (CE-MS) has regained momentum through advances in sensitivity, separation efficiency, and expanding applications in proteomics, metabolomics, and biopharmaceutical analysis. These developments hav...
Plug-in hybrid baculovirus expression vector for high-yield recombinant adeno-associated virus gene therapy vector production [0.03%]
一种重组腺相关病毒载体的高产基因治疗插件式杆状病毒表达载体
Linda A de Jong,Jelmer Willems,Adriaan H de Wilde et al.
Linda A de Jong et al.
In vivo gene therapy is rapidly advancing to treat patients with a wide range of genetic disorders. Recombinant adeno-associated virus (rAAV) vectors provide long-term gene expression, low immunogenicity, and adaptable tissue tropism. Produ...
Redefining biologics safety through advanced analytics: MS-based host cell protein profiling [0.03%]
基于质谱的宿主细胞蛋白谱分析重新定义生物制品安全性
Paresh Tank,Fatima D&#x;Souza,Nagarajan Kayalvizhi et al.
Paresh Tank et al.
Recent regulatory and pharmacopeial guidance highlights attention to host cell protein (HCP) impurities in biologics. Advances in liquid chromatography-mass spectrometry (LC-MS) enable molecular-level characterization of HCPs beyond immunoa...
Beyond natural evolution: multi-scale in vivo mutagenesis toolkits for synthetic evolution [0.03%]
超越自然进化：合成进化的多尺度体内诱变工具包
Zhijun Teng,Julian E Prieto-Vivas,Kevin J Verstrepen et al.
Zhijun Teng et al.
Our current industrial, agricultural, and medical practices exploit the extraordinary biodiversity generated through billions of years of natural evolution. Despite their high fitness in native habitats, biomolecules and organisms are often...