Kaifeng Wang,Mei-Li Sun,Xiao-Jun Ji
Kaifeng Wang
Dynamic regulation enables precise, signal-responsive control of metabolism in oleaginous yeasts, overcoming limitations of static engineering. By leveraging metabolite and spatiotemporal cues, these systems decouple growth from production ...
Upscaled open-culture production of microbial flocculants from industrial wastewaters [0.03%]
基于工业废水的微生物絮凝剂开放式培养生产及其放大研究
Carlos A Contreras-Dávila,Yixin Liu,Dainis Sudmalis et al.
Carlos A Contreras-Dávila et al.
Flocculants are widely used for solid-liquid separation despite environmental risks such as microplastics accumulation or release of toxic compounds. Microbially-secreted biopolymers are potential biodegradable, nontoxic alternatives. We de...
Precision tumor immunotherapy via a dual-gated macrophage-bacterial activation platform [0.03%]
一种双门控巨噬细胞细菌活化平台实现精准肿瘤免疫治疗
Lin Li,Leyang Wu,Liyuan Qiao et al.
Lin Li et al.
Immunotherapy based on live microorganisms has shown promise in preclinical studies, but its clinical translation has been hampered by limited efficacy and non-negligible toxicity. Here, we developed Macrophage-Bacteria encapsulation Lytic ...
Genome-scale CRISPRi and base-editing libraries for genetic decoding and strain engineering in Shewanella [0.03%]
希瓦氏菌遗传密码破译和菌株工程的基因组规模CRISPRi和碱基编辑文库
Yaru Chen,Qiyang Duan,Lin Wang et al.
Yaru Chen et al.
Clustered regularly interspaced short palindromic repeats (CRISPR)-based libraries with diverse gene-editing functions, such as gene knockdown and mutation, can significantly accelerate our understanding of complex metabolic networks in mic...
Jieyu Ge,Tiange Wang,Chengyi Qu et al.
Jieyu Ge et al.
The fungal bioluminescence pathway (FBP) offers a unique and sustainable system for continuous light emission through self-sustaining, nontoxic substrates. Recent advances in FBP-based substrate engineering, enzyme optimization, and metabol...
Ian Stevens,Danielle Pacia,Jan N Hansen et al.
Ian Stevens et al.
Contemporary functional genomics researchers have sought to couple high-throughput experimental technologies with computational methodologies. However, the ethical issues associated with bench science at the early stages of the artificial i...
A durable hydrogel-encapsulated biohybrid for sustainable triclosan bioremediation [0.03%]
一种用于持续灭菌剂生物修复的耐用型水凝胶封装生物杂化材料
Yiran Yin,Hao Ren,Yaxuan Wu et al.
Yiran Yin et al.
Synthetic biology provides an effective approach for emerging contaminant degradation, but biosafety and stability challenges limit its practical use. Here, we engineered Pseudomonas knackmussii ZM30 for efficient triclosan (TCS) degradatio...
Engineering M13 phages: surface and morphological strategies for programmable applications [0.03%]
工程M13噬菌体：面向程序化应用的表面和形态策略
Mingye Song,Jialin Liu,Siqiu Zheng et al.
Mingye Song et al.
Bacteriophage M13 is a structurally ordered filamentous phage that has long served as a versatile bio-nanotemplate for various applications. To adapt to different applications, it is essential to modify and tailor the properties of M13 phag...
Cryobioprinted human tumor models with shelf-stable programmability [0.03%]
可在货架上保存的编程型人体肿瘤 cryobioprinting 技术
Maria V Monteiro,Carlos Ezio Garciamendez-Mijares,David Sebastián Rendon Ruiz et al.
Maria V Monteiro et al.
Cryobioprinting enables simultaneous fabrication and cryopreservation of tissue analogs, surpassing the limitations of print-to-use biofabrication approaches. However, cryopreservation of living constructs remains challenging, requiring opt...
Efficient ergothioneine production through reconstruction of the methyl and sulfur supply systems in Escherichia coli [0.03%]
通过在大肠杆菌中重建甲基和硫供应系统有效生产麦角硫宁
Junjun Yan,Yunhong Chen,Yuyue Ma et al.
Junjun Yan et al.
Ergothioneine (ERG) is a rare amino acid with diverse biological activities, making it valuable for applications in the food, cosmetics, and pharmaceutical industries. Compared with traditional extraction from edible fungi, microbial fermen...