Highly efficient prime editors for mammalian genome editing based on porcine retrovirus reverse transcriptase [0.03%]
基于猪逆转录酶的高效prime编辑器用于哺乳动物基因组编辑
Weiwei Liu,Wenxin Duan,Zhiwei Peng et al.
Weiwei Liu et al.
Prime editing is a versatile and precise genome-editing tool. Most prime editors (PEs) rely on reverse transcriptase (RT) derived from Moloney murine leukemia virus (MMLV). Here, we established a PE, pvPE, using a RT derived from a porcine ...
Engineering infrared light detection in blind human retina using ultrasensitive human TRPV1 channels [0.03%]
利用超灵敏的人类TRPV1通道在盲人视网膜中工程化红外光检测能力
Morgan Chevalier,Firas Fadel,Tímea Májer et al.
Morgan Chevalier et al.
Engineering infrared light sensitivity in the blind human retina could restore visual function in patients with regional retinal degeneration. However, current approaches are complex and contain non-human biological components. Using ration...
Extended human lymph node explants for evaluation of adaptive immunity [0.03%]
延长的人类淋巴结外植体用于评估适应性免疫反应
Kanishka Fernando,Hong Sheng Quah,Lisda Suteja et al.
Kanishka Fernando et al.
Lymph nodes (LNs) are a vital component of the adaptive immune system as they have a key role in antigen presentation and regulation of immune responses. However, preclinical models that accurately mimic the complexity and spatial organizat...
Towards gene editing and torrefaction pretreatment for biochar functionalization [0.03%]
面向基因编辑和热解预处理的生物炭功能化研究
Congyu Zhang,Kuifeng Hao,Wei-Hsin Chen et al.
Congyu Zhang et al.
The global demand for sustainable materials and carbon-neutral technologies drives innovations in biochar, a carbon-rich material for energy storage, pollution mitigation, and carbon sequestration. Conventional biochar, limited by poor cond...
Engineered RNA nanostructures for scalable and efficient RNAi-based pesticides [0.03%]
用于大规模和高效的RNAi农药的工程化RNA纳米结构
Ying Xia,Tao Zhang,Xiaokun Ni et al.
Ying Xia et al.
Double-stranded RNA (dsRNA)-based pesticides face challenges in stability, scalability, efficient uptake, and broad applicability. Here, we present self-assembled RNA nanostructures (SARNs), engineered to load pools of functional siRNAs wit...
Next-generation electroactive biomaterials-integrated bioelectronics for advanced healthcare management [0.03%]
面向高级医疗管理的一代电活性生物材料集成式生物电子器件
Xinhua Liu,Peiyao Huo,Xuechuan Wang et al.
Xinhua Liu et al.
The evolution of smart biomaterials is revolutionizing healthcare through synergistic integration of real-time diagnostic monitoring and regenerative therapies. Current bioelectronic systems demonstrate remarkable capabilities in disease de...
Cansu Pinar Yenice,Ciara K O&#x;Sullivan
Cansu Pinar Yenice
Bridge amplification of nucleic acids exploits the use of surface-tethered forward and reverse primers to allow amplification to occur on a 2D surface, in a spatially controlled manner, facilitating multiplexed reactions for the detection o...
Advanced mass spectrometry techniques for monitoring biopharmaceutical host cell proteins [0.03%]
用于监测生物制药宿主细胞蛋白质的先进质谱技术
Paresh Tank,Shruti Vora,Fatima D&#x;Souza et al.
Paresh Tank et al.
Residual host cell proteins (HCPs) in biologic drug products can compromise safety or stability and must be carefully monitored. While traditional immunoassays remain essential, they often lack specificity or coverage. Mass spectrometry (MS...
Michelle Muzzio
Michelle Muzzio
In a recent study, Johnson et al. demonstrated a potentially generalizable strategy to bioremediate and upcycle plastic waste by interfacing a chemical reaction not typically found in nature with Escherichia coli. They further applied this ...
Milena Mennecozzi,Monica Piergiovanni,Lucia Selfa Aspiroz et al.
Milena Mennecozzi et al.
The EU has been a leader in research and development of in vitro biotechnology (IVB), such as human 3D cell and tissue models used in disease research and drug development. However, it is struggling to convert scientific discoveries into bu...