Strategies for optimizing acetyl-CoA formation from glucose in bacteria [0.03%]
用于优化细菌中由葡萄糖形成乙酰辅酶A的策略
Li Zhu,Jieze Zhang,Jiawei Yang et al.
Li Zhu et al.
Acetyl CoA is an important precursor for various chemicals. We provide a metabolic engineering guideline for the production of acetyl-CoA and other end products from a bacterial chassis. Among 13 pathways that produce acetyl-CoA from glucos...
Bioengineered Microphysiological Placental Models: Towards Improving Understanding of Pregnancy Health and Disease [0.03%]
用于改善妊娠健康及疾病的胎盘微生理模型的生物工程学研究进展
Marnie Winter,Tanja Jankovic-Karasoulos,Claire T Roberts et al.
Marnie Winter et al.
Driven by a lack of appropriate human placenta models, recent years have seen the introduction of bioengineered in vitro models to better understand placental health and disease. Thus far, the focus has been on the maternal-foetal barrier. ...
Engineering Minds? Ethical Considerations on Biotechnological Approaches to Mental Health, Well-Being, and Human Flourishing [0.03%]
工程心灵？论生物技术在心理健康、福祉和人类发展方面的伦理考量
Federico Germani,Philipp Kellmeyer,Sebastian Wäscher et al.
Federico Germani et al.
Our bodies can be designed and modified in accordance with our ideals of health and well-being. These increasingly targeted and personalized interventions will be more effective than current therapies. Here we review technologies to alter m...
Advanced strategies and tools to facilitate and streamline microbial adaptive laboratory evolution [0.03%]
促进和简化微生物适应性实验进化的高级策略和工具
Yinan Wu,Aysha Jameel,Xin-Hui Xing et al.
Yinan Wu et al.
Adaptive laboratory evolution (ALE) has served as a historic microbial engineering method that mimics natural selection to obtain desired microbes. The past decade has witnessed improvements in all aspects of ALE workflow, in terms of growt...
Primate Organoids and Gene-Editing Technologies toward Next-Generation Biomedical Research [0.03%]
灵长类器官和基因编辑技术开启下一代生物医学研究
Chuanxin Chen,Weizhi Ji,Yuyu Niu
Chuanxin Chen
The improved ability to organize pluripotent stem cells (PSCs) into 3D structures in vitro has shed light on organoid technology to recapitulate organs and tumors in vivo. Advances in gene-editing technologies, particularly CRISPR-mediated ...
Hao Gao,Wenming Zhang,Ziyi Yu et al.
Hao Gao et al.
The combination of 3D printing and synthetic biology is a sustainable strategy to fabricate biological objects and systems that behave in a preprogrammed manner. Many microorganisms have been genetically engineered as cell factories for the...
Validation Is Not Verification: Precise Terminology and Scientific Methods in Bioprocess Modeling [0.03%]
验证不等于确认——生物工艺建模的精确术语及科学方法
Jens Smiatek,Alexander Jung,Erich Bluhmki
Jens Smiatek
Advanced statistical approaches and new modeling procedures for biopharmaceutical development and manufacturing have received increasing attention. With this forum article, we would like to highlight the need for a consistent terminology an...
Kelvin C M Lee,Jochen Guck,Keisuke Goda et al.
Kelvin C M Lee et al.
The biophysical properties of cells reflect their identities, underpin their homeostatic state in health, and define the pathogenesis of disease. Recent leapfrogging advances in biophysical cytometry now give access to this information, whi...
Additive Manufacturing Tools to Improve the Performance of Chromatographic Approaches [0.03%]
用于提高色谱法性能的增材制造工具
J F A Valente,F Sousa,N Alves
J F A Valente
Chromatography is widely applied industrially. However, some limitations are associated with its common supports, and the impossibility to fully control their structural features is particularly restrictive. Additive manufacturing (AM) is e...
Leveraging Single-Cell Sequencing for Chimeric Antigen Receptor T Cell Therapies [0.03%]
利用单细胞测序技术促进嵌合抗原受体T细胞治疗的发展
Rocío Castellanos-Rueda,Raphaël B Di Roberto,Fabrice S Schlatter et al.
Rocío Castellanos-Rueda et al.
Chimeric antigen receptor (CAR)-T cell therapies against cancer continue to make inroads in the clinic. However, progress is still hindered by subpar efficacy against many tumors. Gaining a better understanding of CAR-induced T cell activat...