2 H NMR of oriented phospholipid/cholesterol bilayers containing an amphiphilic peptide [0.03%]
含两亲性肽的磷脂/胆固醇双层膜的二维化学位移相关谱研究
Miranda L Schmidt,James H Davis
Miranda L Schmidt
Lipid bilayers form the basis of cell membranes and the phase behaviour of the membrane has been linked to proper cell function. Model membranes composed of relatively simple mixtures of phospholipids and cholesterol can already exhibit com...
Nitrosative stress affects the interaction of integrin alphaIIbbeta3 with its ligands [0.03%]
一氧化氮应激影响整合素alphaIIbbeta3与其配体的相互作用
Sanjai Karanth,Mihaela Delcea
Sanjai Karanth
Binding of integrin alphaIIbbeta3 (αiibβ3) to its ligands is a highly restricted and regulated mechanism. Any modification of the protein structure yields a dysfunctional role, especially in a redox environment. Here, we examine the effec...
Formation of intermediate gel-liquid crystalline phase on medium-chain phosphatidylcholine bilayers: Phase transitions depending on the bilayer packing [0.03%]
中等链磷脂酰胆碱双分子层介观凝胶液晶相的形成：与双分子层堆积相关的相变
Hitoshi Matsuki,Masaki Goto,Makiko Motohashi et al.
Hitoshi Matsuki et al.
The bilayer phase transitions of medium-chain phosphatidylcholines with linear saturated acyl chains (Cn = 12, 13 and 14) were measured by high-pressure light-transmittance measurements and differential scanning calorimetry to investigate t...
The antioxidant vitamin E as a membrane raft modulator: Tocopherols do not abolish lipid domains [0.03%]
抗氧化剂维生素E作为细胞膜微区调节因子：生育酚不会消除脂质微区
Mitchell DiPasquale,Michael H L Nguyen,Brett W Rickeard et al.
Mitchell DiPasquale et al.
The antioxidant vitamin E is a commonly used vitamin supplement. Although the multi-billion dollar vitamin and nutritional supplement industry encourages the use of vitamin E, there is very little evidence supporting its actual health benef...
Rationally designed antimicrobial peptides: Insight into the mechanism of eleven residue peptides against microbial infections [0.03%]
理性设计的抗菌肽：针对微生物感染的十一残基肽作用机制研究
Gopal Pandit,Karishma Biswas,Suvankar Ghosh et al.
Gopal Pandit et al.
The widespread abuse of antibiotics has led to the use of antimicrobial peptides (AMPs) as a replacement for the existing conventional therapeutic agents for combating microbial infections. The broad-spectrum activity and the resilient natu...
Solution structure and oligomeric state of the E. coliglycerol facilitator [0.03%]
大肠杆菌甘油转运蛋白的溶液结构与寡聚状态分析
Mary Hernando,George Orriss,Jacqueline Perodeau et al.
Mary Hernando et al.
Protein dynamics at atomic resolution can provide deep insights into the biological activities of proteins and enzymes but they can also make structure and dynamics studies challenging. Despite their well-known biological and pharmaceutical...
Expression, purification, and functional reconstitution of 19F-labeled cytochrome b5 in peptide nanodiscs for NMR studies [0.03%]
肽纳米盘中表达、纯化并重新构建血红素标记细胞质b5血红素蛋白用于NMR研究
Jia Bai,Jian Wang,Thirupathi Ravula et al.
Jia Bai et al.
Microsomal cytochrome b5 (cytb5) is a membrane-bound protein capable of donating the second electron to cytochrome P450s (cytP450s) in the cytP450s monooxygenase reactions. Recent studies have demonstrated the importance of the transmembran...
Marta Barniol-Xicota,Steven H L Verhelst
Marta Barniol-Xicota
Intramembrane proteases (IMPs) are proteolytic enzymes embedded in the lipid bilayer, where they cleave transmembrane substrates. The importance of IMPs relies on their role in a wide variety of cellular processes and diseases. In order to ...
Styrene maleic-acid lipid particles (SMALPs) into detergent or amphipols: An exchange protocol for membrane protein characterisation [0.03%]
用于膜蛋白表征的 Styrene-Maleic Acid 脂质颗粒（SMALP）到去垢剂或两性氧化物交换方案
Sophie J Hesketh,David P Klebl,Anna J Higgins et al.
Sophie J Hesketh et al.
Membrane proteins are traditionally extracted and purified in detergent for biochemical and structural characterisation. This process is often costly and laborious, and the stripping away of potentially stabilising lipids from the membrane ...
Quantifying the insertion of membrane proteins into lipid bilayer nanodiscs using a fusion protein strategy [0.03%]
融合蛋白策略量化膜蛋白插入脂质双层纳米盘的数量
Elisabeth Häusler,Kai Fredriksson,Inguna Goba et al.
Elisabeth Häusler et al.
A membrane protein's oligomeric state modulates its functionality in various cellular processes. Since membrane proteins have to be solubilized in an appropriate membrane mimetic, the use of classical biophysical methods to analyze protein ...