Topological mapping establishes signal peptide-independent cytoplasmic membrane insertion of bacterial MATE family efflux protein EmmdR [0.03%]
拓扑图表明细菌MATE族外排蛋白EmmDRL的信号肽非依赖性的胞质膜插入性质
Hitkarsh Kushwaha,Jiban Mishra,Harapriya Mohapatra
Hitkarsh Kushwaha
Enterobacter cloacae ATCC13047 encodes the EmmdR efflux protein, which belongs to the Multidrug And Toxic compound Extrusion (MATE) transporter family (Transporter Classification ID 2.A.66.1). The protein has a predicted existence in the Un...
Electrostatics of biomolecular condensates: Methodological advances, limitations, and implications for membrane interactions [0.03%]
生物分子凝聚物的静电学：方法进展、局限性及其对膜相互作用的影响
Agustín Mangiarotti,Natalia Wilke
Agustín Mangiarotti
Biomolecular condensates are vital organizers of cellular space. These membraneless organelles wet and remodel membranes to orchestrate many critical physiological processes. While the molecular interactions driving condensate formation and...
Hydrophilic lipophilic balance: A lighthouse parameter for designing efficient detergents in membrane protein applications? [0.03%]
亲疏水平衡：设计有效表面活性剂以应用于膜蛋白研究的指导参数？
Marine Soulié,Grégory Durand
Marine Soulié
Since the 1970s, the hydrophilic lipophilic balance (HLB) has been widely recognized as a key parameter for evaluating the effectiveness of detergents across various fields, including membrane protein (MP) research. Detergents play a crucia...
Charge-dependent insertion of the antimicrobial peptide magainin 2 into lipid monolayers probed by neutron reflectivity [0.03%]
基于电荷的抗菌肽magainin 2嵌入脂质单分子层的研究：中子反射法探测
Arnaud Marquette,Andrew R J Nelson,Sophie Combet et al.
Arnaud Marquette et al.
Many antimicrobial peptides act by disrupting cellular membranes, with therapeutic selectivity arising from their ability to discriminate between bacterial and mammalian cells, a distinction largely governed by membrane surface charge. Here...
The evolution of steady-state laurdan fluorescence: From model membranes to cellular applications [0.03%]
Laurdan荧光稳态极化演化：从模型膜到细胞应用
Travis Issler,Evan Kerek,Elmar J Prenner
Travis Issler
Laurdan (6-dodecanoyl-2-(dimethylamino)-naphthalene) is a solvatochromic fluorescent probe widely used for investigating membrane biophysical properties. Since its first application in phospholipid bilayers, laurdan has become a valuable to...
Molecular dynamics perspectives on annexin-mediated plasma membrane repair: From recruitment to membrane mechanics [0.03%]
分子动力学视角下的血浆膜修复：从招募到膜力学
Ali Asghar Hakami Zanjani
Ali Asghar Hakami Zanjani
This mini-review examines how molecular dynamics simulations reshape the view of annexin-mediated plasma membrane repair from simple recruitment to coupled protein-membrane states. Molecular dynamics simulations show that annexin-induced cu...
Thermodynamic characterization of chirality effects in dipalmitoylphosphatidylcholine bilayers [0.03%]
二棕榈酰卵磷脂双层膜中手性效应的热力学表征
Masaki Goto,Ririka Matsushita,Maika Yamaguchi et al.
Masaki Goto et al.
The thermodynamic phase behavior of lipid bilayers formed by R-dipalmitoylphosphatidylcholine (R-DPPC), its enantiomer S-DPPC, and racemic DPPC (rac-DPPC) was investigated by differential scanning calorimetry and high-pressure light transmi...
Influence of tail structure on thermotropic phase behavior, stability, supramolecular organization, and vesicle properties of nonionic β-alaninol-based amphiphiles: Homologs of N-acylethanolamines [0.03%]
尾部结构对非离子β-氨基酸型两亲分子的热致相行为、稳定性、超分子组织及囊泡性质的影响：N-烷基乙醇胺衍生物同系物的研究
Dokku Sivaramakrishna
Dokku Sivaramakrishna
The hydrophobic tail structure of lipids or amphiphiles plays a critical role in governing the self-assembly, structure, dynamics, and function. In the present work, β-alaninol-based amphiphiles bearing cholesterol, saturated, unsaturated,...
An inner-pore mutation (F315D) promotes intracellular Mg2+ block and inward rectification of BK channels [0.03%]
内孔通道突变(F315D)促进大电导钙激活钾通道的胞内镁阻滞和整流功能
Anna Sekrecka-Belniak,Piotr Koprowski,Piotr Bednarczyk et al.
Anna Sekrecka-Belniak et al.
Large-conductance Ca2+- and voltage-activated potassium (BK) channels display a near-linear current-voltage relationship in excised membrane patches but exhibit inward rectification in intact cells due to block by intracellular cations. Thi...
Mechanistic evaluation of liposome-amyloid composite nanocarriers for doxorubicin delivery: Linking release kinetics with pharmacokinetic behavior [0.03%]
用于多柔比星递送的脂质体-淀粉样复合纳米载体的机制评价：结合释放动力学与药代动力学行为
Valeriya Trusova,Uliana Malovytsia,Olga Zhytniakivska et al.
Valeriya Trusova et al.
The present study extends our previous experimental investigation of liposome-amyloid composite nanocarriers for doxorubicin (DOX) delivery by providing a mechanistic analysis of how experimentally observed loading and release parameters in...