Regarding Emitter Positioning for Nanoflow Electrospray Ionization with a High-Capacity Inlet Capillary [0.03%]
关于具有高容量入口毛细管的纳米流电喷雾离子化的发射器定位
Noah M Lancaster,Scott T Quarmby,Katherine A Overmyer et al.
Noah M Lancaster et al.
Nanoflow electrospray ionization is commonly used for proteomics due to its high sensitivity. Signal intensity, however, is dependent on optimal emitter positioning relative to the mass spectrometer inlet. Here, we characterize the effect o...
Intramolecularly Labeled Reference Standards of Sulfamethoxazole for Fragment-Specific Isotope Analysis by Electrospray Ionization Orbitrap Mass Spectrometry [0.03%]
内标法标记的磺胺甲恶唑片段同位素定量分析研究（电喷雾四极轨道肼质谱法）
Aoife Canavan,Christopher Dirr,Martin Elsner
Aoife Canavan
The widespread presence of pharmaceuticals, including antibiotics, in our aquatic environment raises important societal concerns. When studying their environmental fate, stable isotope analysis of nitrogen and carbon at natural abundance of...
Efficient and Cost-Effective Enzyme Deposition onto Tissues for Mass Spectrometry Imaging of N-Glycans Using a Mini-Humidifier [0.03%]
使用迷你加湿器高效低成本地将酶沉积到组织中以用于N-糖类的大规模质谱成像技术
Erik Sveen,Alyssa M Moore,Miranda R Weigand et al.
Erik Sveen et al.
Mass spectrometry imaging (MSI) is a powerful technique for studying the spatial localization of N-linked glycans in biological tissues, which are important biomarkers for various diseases. Analyzing N-linked glycans requires the deposition...
Legends of Ion Mobility Spectrometry - Charles S. Harden [0.03%]
离子漂移谱学传奇——Charles S. Harden教授离子迁移谱研究生涯回忆
Maggie Tam,Francisco Fernández-Lima
Maggie Tam
Maggie Tam,Francisco Fernández-Lima
Maggie Tam
Maggie Tam,Francisco Fernández-Lima
Maggie Tam
Mapping Native Peptide-Peptide Noncovalent Interactions: Insights from Trapped Ion Mobility Spectrometry, Electron- and UV-Fragmentation, Tandem Mass Spectrometry, and Molecular Dynamics [0.03%]
离子阱离子淌度质谱和分子动力学揭示的肽-pepptide非共价相互作用映射：自由基解离和二级质谱的见解
Miguel Santos-Fernandez,Kevin Jeanne Dit Fouque,Samuel Silva da Rocha Pita et al.
Miguel Santos-Fernandez et al.
Noncovalent interactions play an important role in the way protein structures and protein-protein interactions are stabilized. Mapping these interactions at a molecular level is crucial as peptide complexes serve as models for protein-prote...
Extending Calibration Anchors Resolves Low- m/ z Shifts in Negative-Mode Orbitrap Workflows [0.03%]
扩展校准参考物质可解决负模式轨道四级杆 workflows 中的低m/z偏移问题
Francisco José Díaz-Galiano,Bruno Le Bizec
Francisco José Díaz-Galiano
Reliable mass accuracy is essential for the confident identification of diagnostic fragment ions in high-resolution mass spectrometry. During the analysis of perfluoroalkyl sulfonic acids (PFSAs) with a Q Exactive Orbitrap, we consistently ...
Isotope Abundance Measurement by Molecular Coulomb Explosion: Proof of Concept and Initial Performance Evaluation for Carbon and Oxygen Isotope Abundance [0.03%]
基于分子库仑爆炸的同位素丰度测量：碳氧同位素丰度定性及初步性能评估
RuiTian Zhang,Chijun Zhang,Liyong Zhang et al.
RuiTian Zhang et al.
Stable and long-lived radioactive isotopes are ubiquitous in nature and serve as unique tracers across diverse fields such as nuclear astrophysics, atmosphere chemistry, hydrology, environmental chemistry, and the diagnosis of diseases in t...
Quantitative Mapping of NHS Ester-Protein Reactivity Using Native Top-Down Mass Spectrometry [0.03%]
利用原位顶向下质谱法定量绘制蛋白质与NHS酯反应性地图
Jack L Bennett,Olivia B Ramsay,Corinne A Lutomski et al.
Jack L Bennett et al.
Covalent ligands are widely used to label, probe, and modulate proteins, but peptide-centric readouts obscure how modifications colocalize on intact proteoforms. This can limit insight into ligand mechanism, modification stoichiometry, and ...