A Universal Buffer System for Native LC-MS Analysis of Antibody-Based Therapeutics [0.03%]
一种用于抗体药物天然LC-MS分析的通用缓冲液体系
Kent R Vosper,Bradley T V Davis,Jagandeep Saraya et al.
Kent R Vosper et al.
Liquid chromatography coupled to mass spectrometry (LC-MS) is a powerful analytical technique for analyzing biological macromolecules. A long-standing challenge has been applying LC-MS at physiological pH under native conditions using volat...
Dissecting Metabolic Rewiring and Gene-Metabolite Interactions by Utilizing Untargeted Metabolomics and Single-Gene Knockouts in the Model Microorganism E. coli [0.03%]
通过在模式微生物E.coli中利用非靶向代谢组学和单基因敲除技术剖析代谢重构及基因-代谢物相互作用
Xinru Pang,Li Chen,Huan Zhang et al.
Xinru Pang et al.
Central carbon metabolism, comprising glycolysis, the tricarboxylic acid (TCA) cycle, and the pentose phosphate pathway (PPP), is essential for Escherichia coli survival and growth. While disruptions in these pathways are known to affect ce...
A Miniature Ion Trap Particle Mass Spectrometer with an Integrated Optical and Charge Detection System [0.03%]
一种具有集成光学和电荷检测系统的微型离子阱粒子质谱仪
Yixin Pan,Caiqiao Xiong,Jinlong Jiang et al.
Yixin Pan et al.
A miniature ion trap particle mass spectrometer with both optical and charge detection systems was constructed in this work. The instrument was initially constructed with an open architecture and mounted on a 600 × 450 mm optical bench. Wi...
Anne Brenner,J D Brookbank
Anne Brenner
Discrimination of Threonine Isomers by Multiple-Stage Tandem Mass Spectrometry with Collision-Induced Dissociation [0.03%]
多重碰撞诱导解离串联质谱的多重阶段分析法在区分苏氨酸异构体中的应用研究
Daiki Asakawa
Daiki Asakawa
Fragmentation of protonated threonine isomers was investigated by multiple-stage tandem mass spectrometry (MSn) with ion-trap collision-induced dissociation (CID) and density functional theory (DFT) calculations. Protonated molecules contai...
A Computational Model for Determining Labeling Duration in Protein Turnover Studies Using a Single Deuterated Water Labeled Sample [0.03%]
一种计算模型：利用单一重水标记样品确定蛋白质周转研究中的标记时间
Henock M Deberneh,Rovshan G Sadygov
Henock M Deberneh
The time course of metabolic labeling using deuterated water, followed by liquid chromatography coupled with mass spectrometry, is employed to investigate the turnover rates of individual proteins in vivo. These labeling experiments are res...
Data-Independent Acquisition (DIA) Strategy for Measuring Protein Stability Using Stability of Proteins from Rates of Oxidation (SPROX) [0.03%]
基于SPROX的DIA策略定量蛋白质稳定性
Natalie M Labbe,Meghan K Virani,Michael C Fitzgerald
Natalie M Labbe
The stability of proteins from the rates of oxidation (SPROX) technique is a mass spectrometry-based approach for making protein folding stability measurements on the proteomic scale. The development and application of SPROX, to date, have ...
Direct Observation of Metastable Fragment Ions in Ultraviolet Photodissociation of Ubiquitin [0.03%]
紫外光解离过程中观测到稳定碎片离子
Fanny C Liu,Jusung Lee,Kaira A Mayberry et al.
Fanny C Liu et al.
Ultraviolet photodissociation (UVPD) of proteins is known to exhibit conformation-dependent fragmentation patterns, but direct structural evidence linking precursor protein and fragment ions has been limited. Here, we apply tandem trapped-i...
Optimization of Radially Segmented Ion Mirrors for High Resolution Charge Detection Mass Spectrometry [0.03%]
高分辨率电荷检测质量质谱的径向分段离子镜优化
David W Reitenbach,Martin F Jarrold
David W Reitenbach
Charge detection mass spectrometry (CD-MS) enables mass measurements to be made for heterogeneous samples into the gigadalton regime. In CD-MS, ions are trapped in an electrostatic linear ion trap (ELIT) where they oscillate back and forth ...
Oligomer-Dependent Gas-Phase Dissociation Behavior of 2-Butanone Peroxide (MEKP) Cations [0.03%]
2-丁酮过氧化物（MEKP）阳离子气相碎裂行为的 oligomer 依赖性
John R Stutzman,Ryan M Bain,Brison A Shira et al.
John R Stutzman et al.
2-Butanone peroxide is a commonly used cross-linker in the chemical industry and a homemade explosive encountered by the forensic community. In this study, the dissociation behavior of ammonium cationized MEKP oligomers was explored using t...