Residue-Level Determination of Small-Molecule-Protein Affinities by Hydrogen-Deuterium Exchange Mass Spectrometry [0.03%]
基于氢氘交换质谱的蛋白质小分子相互作用及亲和力的残基水平研究方法
De Lin,Luma Godoy Magalhaes,Joel McMillan et al.
De Lin et al.
Hydrogen-Deuterium Exchange Mass Spectrometry (HDX-MS) is an established tool in drug discovery, used to characterize target engagement and conformational dynamics, and frequently used in both biopharmaceutical and small molecule drug disco...
Mass Spectrometric Determination of Site-Specific O-Acetylation in Rhamnogalacturonan I Oligomers [0.03%]
关于半乳糖醛酸聚糖I寡糖的O-乙酰化位点的质谱分析研究
Liyanage Devthilini Fernando,Xu Yang,Stephanie Archer-Hartmann et al.
Liyanage Devthilini Fernando et al.
O-acetylation, a common modification in rhamnogalacturonan I (RG-I), is critical for various biological processes, including plant growth, stress responses, and pathogen defense. Precise determination of the degree and specific positions of...
Investigating the Efficiency of Ultraviolet Photodissociation in Peptides Modified with N-Terminal UV-Absorbing Chromophores [0.03%]
带有N-末端吸收染料的肽中紫外光解离效率的研究
Nikita Levin,Yana Demyanenko,Eduardo Kitano et al.
Nikita Levin et al.
Ultraviolet photodissociation (UVPD) has emerged as a powerful alternative to conventional collision-induced dissociation (CID) for peptide and protein sequencing in mass spectrometry-based proteomics. However, the UVPD efficiency depends o...
Anne Brenner,J D Brookbank
Anne Brenner
In Situ GCIB Cryo-Sectioning Enables Subcellular Cryo-ToF-SIMS Imaging of Arabidopsis Seeds [0.03%]
原位GCIB冷冻切片技术使拟南芥种子的亚细胞级冷冻ToF-SIMS成像成为可能
Claire Seydoux,Bérangère Moreau,Michel Boujard et al.
Claire Seydoux et al.
Time-of-flight secondary ion mass spectrometry (ToF-SIMS) enables label-free molecular imaging at submicrometric resolution, but its application to biological samples remains limited due to sample preparation challenges. Conventional fixati...
Investigating the Protonated Cannabinoid Dimer Detected in the Forensic Analysis of Cannabis by DART-MS: A Combined Mass Spectrometry and Computational Study [0.03%]
基于DART-MS的大麻法医分析中检测到的阳离子大麻二聚体的联合质谱和计算研究： cannabinoid二聚体的研究
Parandaman Arathala,Benedetta Garosi,Megan I Chambers et al.
Parandaman Arathala et al.
Direct analysis in real time-high-resolution mass spectrometry (DART-HRMS) enables the rapid chemical profiling of Cannabis samples. This approach reveals a prominent signal at nominal m/z 315, characteristic of protonated isomeric cannabin...
Scalable Bayesian Estimation of Multicondition Omics Networks via Clustering and Iterative Merging [0.03%]
基于聚类和迭代合并的多条件组学网络的可扩展贝叶斯估计方法
Moses Obiri,Erik D VonKaenel,David J Degnan et al.
Moses Obiri et al.
Understanding condition-specific molecular interactions in complex biological systems requires scalable and accurate network inference from high-dimensional omics data, often generated by mass spectrometry platforms. Accurately estimating c...
Mapping the Spatial Distribution of Tryptic Peptides in Formalin-Fixed Paraffin-Embedded Tissues Using Desorption Electrospray Ionization Mass Spectrometry Imaging [0.03%]
基于脱溶剂喷雾电离质谱成像技术绘制福尔马林固定石蜡包埋组织中胰蛋白酶肽段的空间分布图
Chih-Hsiang Chen,Hsin-Hsiang Chung,Yi-Fang Lai et al.
Chih-Hsiang Chen et al.
Spatial proteomics visualizes protein localization within native cellular and tissue environments, providing critical insights into pathological processes, tissue heterogeneity, and diagnostic biomarkers. Mass spectrometry imaging (MSI) has...
Global Profiling of Post-Translationally Modified Crustacean Neuropeptidome Trends Affiliated with Feeding Activity [0.03%]
甲壳动物肽类激素的翻译后修饰及其与摄食行为的关系
Lauren Fields,Kendra G Selby,Meghan M Hayes et al.
Lauren Fields et al.
Neuropeptides modulate a diverse range of physiological functions, including those associated with feeding. Post-translational modifications (PTMs) contribute significantly to the dynamic nature of neuropeptide isoforms, influencing their f...
Evaluating the Sulfo-Phospho-Vanillin Assay for Total Lipid Sample Normalization as Compared to Gravimetric and Protein Sample Normalization Methods for Untargeted Lipidomic LC-MS/MS [0.03%]
与基于重量和蛋白质的样品归一化方法相比，评估磺基磷酸香草素测定法用于脂质组LC-MS/MS中总脂质样品归一化的效果
Laura S Bailey,Kari B Basso
Laura S Bailey
Sample normalization is essential for lipid quantitation. While normalization methods involving pre- or postgravimetric measurements, counting, or protein prequantitation exist, a single, common sample normalization method is not widely acc...