Interdomain Interactions Modulate Refolding Kinetics and Aggregation in a Monoclonal Antibody [0.03%]
不同域间的相互作用调控单克隆抗体的重折叠动力学及聚集行为
Philipp Trolese,Andrea Pierangelini,Benedetta Fongaro et al.
Philipp Trolese et al.
Understanding the structural determinants of antibody stability and aggregation is essential for therapeutic development. In this study, we investigated the unfolding and refolding behavior of bevacizumab under denaturing conditions using d...
Side Chain Driven Mass Spectral Fragmentation of Lys Containing Peptides: Cα-CO Bond Cleavage in Xxx-Lys-Xxx Sequences [0.03%]
侧链驱动的lys残基肽段质谱碎裂：Xxx-Lys-Xxx序列中的Cα-CO键断裂
Sanjeev Kumar,Marimuthu Vijayasarathy,Venkatesha M Achanna et al.
Sanjeev Kumar et al.
Mass spectral fragmentation of the tripeptide amide Ala-Lys-Ala-amide (AKA*) yields a product ion at m/z 228.1, which corresponds to a neutral loss of 43 Da from the b3 ion (m/z 271.1). Similar losses of 43 Da are observed from bn ions in t...
Detection and Characterization of Cyanometabolite Complexes between Microcystin-RR and a Microguanidine by UHPLC-ESI-MS [0.03%]
超高效液相色谱-电喷雾质谱检测和表征微囊藻毒素-LR与微胍的氰化物复合物
Sanduni H Premathilaka,Dragan Isailovic
Sanduni H Premathilaka
Microcystins (MCs) are hepatotoxic cyclic peptides produced by cyanobacteria, with MC-RR being one of the most polar and commonly detected MC congeners in water collected during cyanobacterial harmful algal blooms (cHABs). Microguanidines (...
Active Humidity Control Chamber for Desorption Electrospray Ionization-Mass Spectrometry Imaging Applications [0.03%]
用于解吸电喷雾离子化-质谱成像的主动湿度控制舱
Hawkins S Shepard,Robert L G Gottschalk,Jody C May et al.
Hawkins S Shepard et al.
Ambient ionization techniques enable mass spectrometry (MS) to expand into broader experimental contexts, although it is increasingly clear that results are influenced by the environmental conditions at the site of sampling. Desorption elec...
Combined Trapped Ion Mobility and Infrared Ion Spectroscopy Study of Protonation Sites in Aromatic Amines [0.03%]
结合限制型离子淌度和红外离子光谱研究芳香胺的质子化位点
Laura Finazzi,Lara van Tetering,Jelle L Schuurman et al.
Laura Finazzi et al.
The protonation site of aromatic amines in the gas phase has been under substantial debate, as it involves a subtle competition between the higher electronegativity of the amine nitrogen and the better charge delocalization ability of the f...
Collision-Induced Unfolding of High- m/ z Native-like Protein Ions within a Trapped Ion Mobility Spectrometer [0.03%]
高m/z天然类蛋白离子在捕集漂移管离子淌度仪中的碰撞诱导去折叠
Olakunle O Akinola,Nicholas B Borotto
Olakunle O Akinola
Native mass spectrometry (nMS) is a powerful tool for the rapid characterization of protein ions and protein-ligand complexes. By coupling nMS with ion mobility spectrometry (IMS), and collisional activation, we can rapidly obtain insights ...
Anne Brenner,J D Brookbank
Anne Brenner
Multipass CCS Refiner: A Web Application for Accurate Collision Cross Section Calibration in Cyclic Ion Mobility-Mass Spectrometry [0.03%]
多功能CCS精炼器：一种用于环形离子移动-质谱仪中准确碰撞截面校准的网络应用
Eric C Gier,Dmitry Leontyev,Facundo M Fernández
Eric C Gier
In this paper, we present the Multipass CCS Refiner, an R application for calibrating multipass collision cross section (CCS) measurements with cyclic ion mobility spectrometry (cIMS). The Multipass CCS Refiner combines the necessary tools ...
Anne Brenner,J D Brookbank,Kylie Bemis
Anne Brenner
The Protective Effects of CO2 on Fragile Ions in Differential Mobility Spectrometry [0.03%]
二氧化碳对差示移动谱中脆弱离子的保护效应
Andrew L Finlay,Wojciech Gabryelski,W Scott Hopkins
Andrew L Finlay
We explore the protective effects of adding CO2 to the N2 carrier gas when we conduct differential mobility spectrometry (DMS) analysis of fragile ions. A selection of fragile analytes of varying chemistries were chosen from our lab invento...