An Online Buffer Exchange Platform for Charge Detection Mass Spectrometry Analysis of AAVs and AAV-Antibody Complexes [0.03%]
一种在线缓冲液交换平台 用于AAV及其抗体复合物的电检测质谱分析
Chen Du,Victoria C Cotham,Garima Thakur et al.
Chen Du et al.
Adeno-associated viruses (AAVs) are leading vectors in gene therapy that have demonstrated great potential in combating a wide range of human diseases. To enhance specificity and reduce dosing requirements, antibody-retargeted AAVs have eme...
Characterization of Micrococcus luteus Lipidome Containing Novel Lipid Families by Multiple Stage Linear Ion-Trap with High Resolution Mass Spectrometry [0.03%]
采用高分辨率质谱多级线性离trap技术表征含有新型脂质家族微球菌脂质组
Brian A Kleiboeker,Fong-Fu Hsu
Brian A Kleiboeker
Micrococcus luteus (M. luteus) is a ubiquitous, long-existing Gram-positive bacterium with a distinctive yellow or golden color. It is a model organism for laboratory studies due to its small genome and ease of cultivation. However, only li...
Profiling Multiple Steroid Subclasses Enabled by Rounded Turn SLIM Ion Mobility [0.03%]
通过圆角转弯SLIM离子淌度表征多种固醇亚类
Cole L Frank,Breland M Jones,Emmaleigh Efird et al.
Cole L Frank et al.
Ion mobility-mass spectrometry has shown tremendous potential for improving the analysis of various subclasses of steroids. Its speed and ability to separate isobaric and isomeric species makes it ideal for biomedical, clinical, food, envir...
Relational Graph Convolutional Network for Robust Mass Spectrum Classification [0.03%]
鲁棒性质谱分类的图卷积神经网络
Raphaël La Rocca,Anthony Cioppa,Enrico Ferrarini et al.
Raphaël La Rocca et al.
Supervised machine learning methods have shown impressive performance in interpreting mass signals and automatically segmenting spatially meaningful regions in Mass Spectrometry Imaging (MSI). Such segmentation generates maps that provide r...
Effect of pH on the Kinetics of Cysteine-to-Cystine Conversion in Thin Films Formed by ESI Microdroplet Deposition [0.03%]
pH对电喷雾微滴沉积胱氨酸薄膜半胱氨酸转化动力学的影响
Marta Managò,Chiara Salvitti,Anna Troiani et al.
Marta Managò et al.
The oxidation of cysteine to cystine was investigated in aqueous thin films generated by the deposition of electrospray ionization (ESI) microdroplets. The confined volume of the thin film promotes the reaction, resulting in up to 80% conve...
Electron Capture Dissociation for Discovery Top-Down Proteomics of Peptides and Small Proteins on Chromatographic Time Scales [0.03%]
基于电子捕获解离的高效液相色谱尺度的肽和小蛋白质的由上至下的 top-down proteome 定量分析方法
Lester S Manly,Anne M Roberts,Joseph S Beckman et al.
Lester S Manly et al.
Bottom-up proteomics introduces proteoform ambiguity due to the loss of connectivity between peptides and their original proteoforms. Top-down proteomics (TDP) removes the ambiguity through the direct identification and characterization of ...
Simulating Collision-Induced Dissociation Tandem Mass Spectrometry (CID-MS/MS) for the Blood Exposome Database Using Quantum Chemistry Methods - A Pilot Study [0.03%]
用于血暴露组数据库的碰撞诱导解离串联质谱（CID-MS/MS）的量子化学法模拟初步研究
Jesi Lee,Dinesh Barupal
Jesi Lee
A significant number of compounds in exposome databases and chemical inventories lack mass spectral data due to the nonavailability of reference standards. To address this limitation, computational chemistry methods can be utilized to exten...
Optimization of q Value for Sensitive Detection of Uridine and Thymidine Nucleosides by MS3 [0.03%]
MS3优化以实现尿嘧啶和胸腺嘧啶核苷的高灵敏度检测
Zheng Duan,Chen Wang,Yinsheng Wang
Zheng Duan
Liquid chromatography-tandem mass spectrometry has been widely used to quantify modified nucleosides enzymatically released from RNA and DNA. Compared to MS/MS, MS3 offers higher specificity for analytes in complex sample matrices because c...
Capturing Current Practices and Characterizing Measurement Reproducibility for Seized Drug Analysis Using Ambient Ionization Mass Spectrometry: An Interlaboratory Study [0.03%]
用于缴获毒品分析的 ambient mass 离子化质谱当前实践和测量可重复性表征的实验室间研究
Edward Sisco,Dennis D Leber,Arun S Moorthy
Edward Sisco
The use of ambient ionization mass spectrometry (AI-MS) to aid in the preliminary screening of seized drug evidence has steadily increased over the past two decades. Unlike gas chromatography-mass spectrometry (GC-MS), where electron ioniza...
Absolute Quantitation of Coeluting Impurities in Peptide Drugs Using High Resolution Mass Spectrometry: Glucagon a Case Study in Pharmaceutical Development [0.03%]
利用高分辨率质谱进行肽类药物共洗脱杂质的绝对定量：以胰岛素样生长因子为例的研究与开发工作
Anusha Kodidasu,Koushik Acharyya,Vasanthakumar Ganga Ramu
Anusha Kodidasu
Synthetic peptide-based drugs provide customized therapeutic solutions, but developing a peptide medicine presents various challenges, especially in terms of impurity management. This holds true when traditional techniques like RP-HPLC fail...