A Molecular Fragment Database Generated through Simulated Sequential Single-Bond-Breaking [0.03%]
一种通过模拟连续断键生成的分子碎片数据库
Jesse Fraser,Arun S Moorthy
Jesse Fraser
We implemented a straightforward algorithm for generating a list of potential molecular fragments from a given molecule by simulating sequential fracturing of single bonds; we refer to this approach as the Simulated Sequential Single-Bond-B...
MALDI-TOF MS Detection of Oxidized Major Phospholipids in Biological Samples Using Conventional Matrices and 1-Pyrenebutyric Hydrazide [0.03%]
应用常规基质和1-pirenebutyric hydrazide检测生物样品中的主要氧化磷脂MALDI-TOF MS法
Patricia Prabutzki,Jürgen Schiller,Kathrin M Engel
Patricia Prabutzki
Oxidized lipids are involved in many widespread diseases associated with dysregulated lipid metabolism and/or low-level chronic inflammation. An increase in reactive oxygen species due to redox imbalance leads to the generation of various l...
Detection of Gas-Phase Formaldehyde via C2H5O+ Produced by VUV Illumination and Enhanced by Doping Propionaldehyde [0.03%]
通过VUV照射生成并加入丙醛增强C2H5O+以检测气相甲醛的方法
Zhiyang Wei,Lixin Shan,Yongze Gao et al.
Zhiyang Wei et al.
Formaldehyde (CH2O) is one of the main indoor organic pollutants due to its carcinogenicity and teratogenicity. However, CH2O has been an undetectable species for common VUV lamp-based photoionization mass spectrometry (PI-MS) due to its hi...
Site-Specific Hydrogen Deuterium Exchange Difference Mass Spectrometry Measurements for Ligand Binding [0.03%]
配体结合的位点特异性氢氘交换差示质谱测量
Joseph Anacleto,Suzanne Ackloo,Cheryl Arrowsmith et al.
Joseph Anacleto et al.
Conventional bottom-up HDX-MS experiments are highly suitable for use in drug development; however, a major limitation of this approach is that it generally provides only peptide-level structural resolution. Site specific (i.e., single amin...
Mass Spectrometric Exploration of Biclonal Gammopathy: Insights from a Diagnostic Laboratory [0.03%]
双克隆单株球蛋白血症质谱分析的诊断和挑战
Deepalakshmi D Putchen,Ankitha K Puthiyaveettil,Prajwal Ammalli et al.
Deepalakshmi D Putchen et al.
Biclonal gammopathy is observed in approximately 1% of myeloma cases. Most current understanding of this condition is derived from incidental or anecdotal findings based on serum protein electrophoresis (SPEP) and immunofixation electrophor...
Cysteine Reactivity Profiling Illuminates Monoclonal Antibody Disulfide Bond Reduction Mechanisms in Biopharmaceutical Process Intermediates [0.03%]
半胱氨酸反应性谱分析阐明生物制药中间体单克隆抗体二硫键还原机制
Taku Tsukidate,Zhenshu Wang,Andrew Hsieh et al.
Taku Tsukidate et al.
Monoclonal antibodies (mAbs) are crucial biotherapeutics in the face of increasing global demand, but their production can be impacted by the reduction of disulfide bonds. This study presents a chemical proteomics workflow aimed at elucidat...
Insight into Desorption Mechanisms in a Helium Low-Temperature Plasma Ionization Source Using Computational Simulations [0.03%]
基于计算模拟的氦低温等离子体电离源解吸机理研究
Odhisea Gazeli,Constantinos Lazarou,Marcos Bouza et al.
Odhisea Gazeli et al.
Understanding desorption mechanisms is essential for the optimization of analytical techniques that enable the direct sampling and ionization of condensed-phase samples without preparation. The low-temperature plasma (LTP) ionization source...
Heidi M Sabatini,Christopher D Chouinard
Heidi M Sabatini
The detection and differentiation of explosive compounds remain critical components of security applications and forensic work due to the potential complexity of samples and presence of isomeric compounds. Standalone ion mobility spectromet...
Andrew R Forero,Samuel A Miller,Lilian Valadares Tose et al.
Andrew R Forero et al.
The escalating prevalence and diversity of fentanyl analogues poses an immediate concern for the global community. Fentanyl and its analogues are the primary contributors to both fatal and nonfatal overdoses in the United States. The most r...
Impact of Charge Reduction on Sequence Coverage of Proteins by 193 nm Ultraviolet Photodissociation [0.03%]
电荷减少对193纳米紫外光解碎裂蛋白质序列覆盖的影响
Sean D Dunham,Mohamed I Gadallah,Jennifer S Brodbelt
Sean D Dunham
An ongoing goal of top-down mass spectrometry is to increase the performance for larger proteins. Using higher energy activation methods, like 193 nm ultraviolet photodissociation (UVPD), offers the potential to cause more extensive fragmen...