High-Speed Automated Microdroplet Reactions with Ion-Mobility for Rapid Therapeutic Protein Characterization [0.03%]
用于快速治疗蛋白表征的高速自动微滴反应及离子移动性技术
Krishna D B Anapindi,Edward J Hsieh,Thomas Walker et al.
Krishna D B Anapindi et al.
In the field of protein therapeutic discovery, it is crucial to characterize molecules quickly and precisely, while using minimal sample amounts. Traditional techniques often require significant amounts of samples (10-20 μg) and can introd...
A Novel C-terminal Sequence Variant Discovered in an IgG1 Monoclonal Antibody by LC-MS [0.03%]
一种在IgG1单克隆抗体上由LC-MS发现的新型C端序列变异体
Oliver Silerio,Miyang Li,Douglas S Rehder et al.
Oliver Silerio et al.
Sequence variant analysis is critical for characterizing biologic therapeutics and ensuring product quality, consistency, and safety. During routine tryptic peptide mapping of an IgG1 monoclonal antibody therapeutic, we identified a novel v...
Molecular Dynamics of Neurotensin and Lysozyme Soft-Landing on Gold: Influence of the Molecular Projectile Velocity, Incidence Angle and Temperature [0.03%]
神经肽神经紧张素和溶菌酶轰击金表面的分子动力学研究：分子射弹速度、入射角和温度的影响
Samuel Bertolini,Arnaud Delcorte
Samuel Bertolini
Employing large argon cluster ion beams, the iBEAM technique has exhibited aptitude in transferring large intact biomolecules from a target to a collector surface in the vacuum, e.g., lysozyme and glucose oxidase, while preserving their bio...
LCMS/MS-Based Mining of the Peptaibiome: A Discovery of New Peptaibols [0.03%]
基于LC-MS/MS的肽酰轮库挖掘：新肽类化合物的发现
Nisha Sharma,Poonam Choudhary,Priya Kumari et al.
Nisha Sharma et al.
In this study, we employed liquid chromatography-tandem mass spectrometry (LC-MS/MS) to perform chemo-profiling on four fungal species: three Trichoderma species─two strains of Trichoderma asperellum (S3-3 and S3-1) and one Trichoderma lon...
Targeted Metabolomics of Tityus Scorpion Venoms: Unveiling Small-Molecule Components [0.03%]
基于目标的多重金蜘蛛蝎毒代谢组研究：小分子组分揭露
Nathalia Baptista Dias,Bibiana Monson de Souza,Geovanny Barroso et al.
Nathalia Baptista Dias et al.
Scorpion venoms consist of proteins, peptides, and various low molecular weight (LMW) organic compounds, which act as toxins. Despite their potential significance, these compounds in scorpion venoms have been little investigated and their f...
UV-Vis Action Spectroscopy Reveals Structures of DNA Codon Trinucleotide Cation Radicals [0.03%]
紫外光谱揭示DNA密码子三核苷酸阳离子自由基结构
Frantisek Tureček,Shu R Huang,Yue Liu
Frantisek Tureček
We report a comprehensive spectroscopic study of gas-phase cation radicals and dication radicals derived from DNA trinucleotide codons AAA, AAC, AAT, ATA, and TAA that was augmented by computational analysis of protomer and conformer struct...
Performance Evaluation of Constant Field and Traveling Wave-Based Structures for Lossless Ion Manipulations Devices Coupled to Mass Spectrometry [0.03%]
用于与质谱仪耦合的无损离子操控器件的恒定场和行波结构性能评估
Christopher P Harrilal,Isaac K Attah,Pearl Kwantwi-Barima et al.
Christopher P Harrilal et al.
The performances of three prototype structures for lossless ion manipulations IMS mass spectrometer (SLIM IMS-MS) are evaluated. The instrument was constructed by replacing the drift tube of an Agilent 6560 with either traveling wave (TW) o...
Proteoform Characterization of HIV-1 Broadly Neutralizing Antibody PGT 121.414.LS Product Through Middle-Up and Bottom-Up Proteomics for Clinical Support [0.03%]
临床支持艾滋病病毒广谱中和抗体PGT 121.414.LS产物的蛋白质组学研究
Connor E Gould,Qing Ma,Raymond Cha et al.
Connor E Gould et al.
Broadly neutralizing antibody (bNAb) therapies are under development for the prevention and treatment of HIV-1 infection as an alternative to conventional antiretroviral therapy because of their potential for sustained passive immunotherapy...
An Integrated Strategy for Rapid Profiling of Depsipeptides by High-Performance Liquid Chromatography Coupled to Quadrupole Time-of-Flight Mass Spectrometry: Bombyx batryticatus as an Example [0.03%]
高效液相色谱-四极杆飞行时间质谱联用技术快速剖析并表征天然多肽类成分的整体解决方案——家蚕生物转化研究为例
Huijie Sun,Yang Li,Yunhua Feng et al.
Huijie Sun et al.
Depsipeptides, a structurally diverse class of nonribosomal peptides with broad bioactivities, present significant challenges for systematic characterization due to their complex fragmentation patterns in mass spectrometry (MS). This study ...