Top-Down Mass Spectrometry of a Clinical Antibody Light Chain Using the Omnitrap-Orbitrap-Booster Platform [0.03%]
基于Omnitrap Orbitrap Booster平台的临床抗体轻链自上而下质谱分析
Camille Garcia,Nina A Khristenko,TingTing Fu et al.
Camille Garcia et al.
The Omnitrap-Orbitrap-Booster (OOB) mass spectrometry (MS) platform was developed to advance the top-down (TD) MS analysis of proteins. It integrates a multimodal tandem mass spectrometry (MS/MS) ion trap system (Omnitrap), a high-resolutio...
The 3rd International Top-Down Proteomics Symposium Brings Proteoform Biology to the Forefront of Proteomics [0.03%]
第三届国际自上而下蛋白质组学研讨会将蛋白形式生物学推向蛋白质组学的最前沿
Paul O Danis,Joseph A Loo,Hartmut Schlüter
Paul O Danis
Rapid Glass Wool Enrichment of Glycopeptides for MALDI-MS Analysis of Immunoglobulin G Glycosylation in COVID-19 Samples [0.03%]
用于COVID-19样本中免疫球蛋白G糖基化MALDI-MS分析的快速玻璃棉富集法测定糖肽
Yuye Zhou,Felicia Karlahag,Sophia Schedin Weiss et al.
Yuye Zhou et al.
Glycosylation is the most common protein post-translational modification, affecting protein properties and functions. Abnormal variations in glycosylation are associated with diseases, e.g., coronavirus disease COVID-19. Matrix-assisted las...
Enhanced LC-MS Detection of γ-Aminobutyric Acid via Trimethylation Enhancement Using Diazomethane [0.03%]
重氮甲烷三甲基化增强法提高γ-氨基丁酸的液相色谱-质谱检测灵敏度
Christian A Rosales,Noah A Lepinsky,Mohamed Ramadan et al.
Christian A Rosales et al.
Neurotransmitters are critical for the proper function, signal transmission, and physiological balance of the brain, with γ-aminobutyric acid (GABA) being the main inhibitory neurotransmitter in the central nervous system. GABA is present ...
Assessing the Impact of CDR Deamidation and Isomerization on mAb Target Binding Using Affinity-Resolved 2D-SEC-SCX-MS Analysis [0.03%]
采用亲和力解析的二维尺寸排阻阴离子交换质谱法评估CDR脱氨和异构化对抗体药物结合力的影响
Yuetian Yan,Shuli Tang,Shunhai Wang et al.
Yuetian Yan et al.
Post-translational modifications (PTMs) within the Complementarity-Determining Regions (CDRs) of monoclonal antibodies (mAbs) can impact their target-binding capabilities, making them potential critical quality attributes (CQAs) during ther...
Elucidating the Protective Mechanisms of Methimazole against Copper(I)-Mediated Fenton Chemistry Using Online Mass Spectrometry [0.03%]
基于在线质谱的甲巯咪唑对铜(I)介导的芬顿反应保护机制研究
Yun Li,Ruijin Pu,Meng Su et al.
Yun Li et al.
Methimazole (MMI), which is primarily known as an antithyroid drug, has received considerable attention for its potent antioxidant properties in a copper(I)-mediated Fenton reaction. However, the antioxidant mechanistic details of MMI, part...
Effects of Inlet Capillary Temperature in Atmospheric-Pressure Infrared Laser-Ablation Plasma Postionization Mass Spectrometry [0.03%]
大气压红外激光解吸等离子体后续电离质谱的入射毛细管温度效应
Lilian Ellis-Gibbings,Rory T Steven,Alex J Dexter et al.
Lilian Ellis-Gibbings et al.
Mass spectrometry imaging (MSI) can be used to survey numerous molecular species from a wide variety of surfaces, including biological tissue sections. Atmospheric-pressure (AP) infrared laser-ablation plasma postionization (IR-PPI) has rec...
Theoretical Study Synchronized Reverse Scan Collision-Induced Dissociation in Digital Linear Ion Trap [0.03%]
数字线性离子阱同步反向扫描碰撞诱导解离的理论研究
Weimin Wang,Zhichao Xie,Fuxing Xu et al.
Weimin Wang et al.
The effectiveness of collision-induced dissociation (CID) in ion trap mass spectrometry (ITMS) is limited by a low-mass cutoff and weak fragmentation yields. Theoretically, the q value is optimized to balance the fractional product ion mass...
Investigating the Quantitative Structure-Ionization Efficiency Relationship of Small Molecules and Lipids in the Presence of Ammonium Fluoride in MALDI-TIMS-QTOF Mass Spectrometry Imaging [0.03%]
含氟化铵条件下的基质辅助激光解析电离-飞行时间质量密度成像中，小分子和脂质的定量结构-离子化效率关系研究
Varvara Nikolopoulou,Arthur Stem,Vasilis Vasiliou et al.
Varvara Nikolopoulou et al.
Metabolites are essential small molecules that are naturally occurring in biological processes as end or intermediate products of various pathways. Matrix-assisted laser desorption/ionization-trapped ion mobility separation-mass spectrometr...
Development and Modification of Ion Mobility Mass Spectrometry Instrumentation for Enhanced Analysis of Biological Molecules [0.03%]
用于增强生物分子分析的离子淌度质谱仪器的发展与改进
Jamie P Butalewicz,Jennifer S Brodbelt
Jamie P Butalewicz
Ion mobility separates ion in the gas phase based on rotationally averaged cross section, a parameter often correlated with size, providing a versatile measurement strategy when integrated with mass spectrometry. The rapid growth in the fie...