ExD vs EThcD: What's Better for the Direct Sequencing of Endogenous Amphibian Disulfide Peptides [0.03%]
ExD与EThcD：哪种方法更适合直接测定内源两栖动物二硫键肽类物质
Dmitrii M Mazur,Tatiana Yu Samgina,Yury V Vasil&#x;ev et al.
Dmitrii M Mazur et al.
Intact amphibian skin peptides, apart from their intrinsic interest, are a challenging model system to demonstrate direct sequencing, avoiding any preliminary derivatization steps. They are relatively long (up to 46-mer), contain an intramo...
High-Throughput Quantitation of Plasma Trimethylamine N-oxide Using Desorption Electrospray Ionization Mass Spectrometry for Rapid Cardiovascular Disease Screening [0.03%]
利用解吸电喷雾离子化质谱技术定量血浆三甲胺N-氧化物的高通量方法，用于快速心血管疾病筛查
Kai-Yuan Chiu,Yun-Chen Hsieh,Hsin-Bai Zou et al.
Kai-Yuan Chiu et al.
Trimethylamine N-oxide (TMAO) is an emerging biomarker of cardiovascular disease (CVD) risk, but current detection methods are limited by low throughput and lengthy workflows. To address this, we developed a high-throughput desorption elect...
High-Throughput Mass Spectral Library Searching of Small Molecules in R with NIST MSPepSearch [0.03%]
基于R语言和NIST的MSPepSearch的小分子高通量质谱库检索
Andrey Samokhin,Mikhail Khrisanfov
Andrey Samokhin
High-level programming languages such as Python and R are widely used in mass spectrometry data processing, where library searching is a standard step. Despite the availability of numerous library search algorithms, those developed by NIST ...
James Denholm,Lucy E Flint,Jack Richings et al.
James Denholm et al.
Mass spectrometry imaging is a powerful technique which maps the spatial distribution of thousands of biomolecules across tissue sections. The clear delineation of tissue is an important preceding analysis step typically requiring manual in...
Reducing Mass Spectrometry Noise via Coupled Desorption Flux and Background Modeling [0.03%]
基于解吸通量和背景噪声建模的质谱去噪方法研究
David W Dye,John M Alred,William A Hoey et al.
David W Dye et al.
The identities and outgassing rates of contaminants associated with a material determine its suitability for space applications. Thermogravimetric analysis (TGA) is one test commonly used for evaluating these material properties. During TGA...
In-Solution Conformation Dynamics of Hemoprotein Catalytic Adaptability Revealed by Ultraviolet Photodissociation Mass Spectrometry [0.03%]
紫外光解离质谱揭示蛋白质溶液中血红蛋白催化适应性构象动态变化机制
Pan Luo,Dai Zhang,Chang Liu et al.
Pan Luo et al.
Despite structure-based mutagenesis being widely used for the rational evolution of engineering enzymes, the in-solution conformation dynamics of enzyme catalytic adaptability is still hard to profile and modulate. Herein, we utilize native...
Improving Metabolite Annotations in On-Tissue Chemical Derivatization Mass Spectrometry Imaging by Functional Group Filtering and Hydrogen-Deuterium Exchange [0.03%]
通过功能基团筛选和氢氘交换改善组织原位化学衍生化质谱成像中的代谢标注
Anna Uhlmansiek,Josiah J Rensner,Young Jin Lee
Anna Uhlmansiek
Matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI) enables the direct visualization of metabolites from tissue sections with high spatial resolution. However, its application to untargeted spatial metabolomics...
Characterization of Mobility-Dependent Ion Confinement in Rotating Electric Fields [0.03%]
旋转电场中与离子迁移率相关的限制行为研究
Jung Yun Lee,Sandilya V B Garimella,Randolph V Norheim et al.
Jung Yun Lee et al.
Here, we describe ion confinement based on mobility in rotating electric fields under low E/N conditions. To do this, we constructed a device with a stack of eight segmented ring electrodes to which sinusoidal waveforms were applied with a ...
Hyphenation of Trapped Ion Mobility to Two-Dimensional Mass Spectrometry for Protein Analysis in Complex Biomixtures [0.03%]
用于复杂生物样本中蛋白质分析的二维质量谱与被束缚离子漂移技术的结合
Callan Littlejohn,Meng Li,Christopher A Wootton et al.
Callan Littlejohn et al.
The analysis of complex biological mixtures remains a significant challenge in mass spectrometry (MS), particularly when using conventional direct infusion MS/MS approaches due to inherent limitations in resolving power and spectral complex...
Sensitive and Selective Quantification of Acrylamide in French Fries and Canola Oil by Fluorous Derivatization with Liquid Chromatography-Tandem Mass Spectrometry [0.03%]
采用全氟化衍生液相色谱-串联质谱法灵敏、选择地测定法式薯条和菜籽油中丙烯酰胺含量
Shimba Kawasue,Takahisa Shigematsu,Kento Sonogi et al.
Shimba Kawasue et al.
Acrylamide is a carcinogen produced when foods containing sugar and asparagine are heated to 120 °C or higher during thermal cooking. As efforts are underway globally to reduce acrylamide intake, simple and practical methods to determine i...