Implementation of Infrared-Activated Negative Electron Transfer Dissociation (IR-NETD) Using Xenon on a Quadrupole-Orbitrap-Quadrupole Linear Ion Trap Mass Spectrometer [0.03%]
基于四极杆-轨道阱-四极杆线性离子阱质谱仪的氙灯红外负电子转移解离技术（Xe-IR-NETD）的应用与实现
Daniel J Nesbitt,Keaton L Mertz,Mitchell D Probasco et al.
Daniel J Nesbitt et al.
For tandem mass spectrometry, photoactivation capabilities enable a host of useful dissociation strategies. Herein we present the first implementation of an IR laser system on a next generation, quadrupole-Orbitrap-quadrupole linear ion tra...
Improving Peak Capacity in Glycan Ion Mobility Separations through Traveling Wave-Based Ion Heating [0.03%]
基于旅行波的离子加热在糖类离子淌度分离中提高峰容量
Megan R Rooney,Gabe Nagy
Megan R Rooney
Complex carbohydrates, such as N-linked glycans, are highly important biomolecules with roles ranging from signaling to recognition and immune response. Ion mobility spectrometry-mass spectrometry (IMS-MS) has emerged as a rapid and orthogo...
IscS Kinetics in Native Mass Spectrometry Buffers Reveal Key Physiochemical Properties that Influence Enzyme Activity [0.03%]
原位质谱缓冲液中ISCS动力学揭示影响酶活性的关键理化性质
Shelby D Oney-Hawthorne,David P Barondeau,David H Russell
Shelby D Oney-Hawthorne
Investigations of protein function and interactions with native mass spectrometry (MS) have yielded significant insights into protein dynamics, transient reaction intermediates, and pharmacokinetic targets. The pursuit of these studies and ...
Separation of Epimers of a Cleavable ADC Drug Linker Using SLIM-Ion Mobility Mass Spectrometry [0.03%]
利用SLIM-离子淌度质谱分离可裂解的ADC药物连接子的差向异构体
Meenakshi Goel,Sayyeda Zeenat Razvi,Sreya Sarkar et al.
Meenakshi Goel et al.
Antibody-drug conjugates (ADCs) represent a rapidly evolving class of potent biopharmaceuticals, combining the selective targeting of monoclonal antibodies with the cytotoxic power of small-molecule drugs. These targeted cancer therapies ar...
Repurposing a Q-ToF Collision Cell into a Stacked-Ring Ion Trap for Controlled Ion-Molecule Reactions with Minimal Heating [0.03%]
将Q-ToF碰撞池改造为堆叠式离子阱以进行受控的低加热离子-分子反应
Chong Zhang,Jake P Violi,Christopher S Hansen et al.
Chong Zhang et al.
Ion-molecule reactions at near-ambient temperatures provide a controlled means to study gas-phase interactions that underpin fundamental chemical processes, such as binding, molecular recognition, and reactivity. Here, we demonstrate that s...
Stress-Induced Antibody Aggregates: Insights from Native SEC-MS with Postcolumn Denaturation [0.03%]
应力诱导的抗体聚集体：源自天然SEC-MS带柱后的变性分析的新见解
Daniil Ivanov,Christine Lee,Dennis Delgado et al.
Daniil Ivanov et al.
Characterization of high-molecular-weight species (HMWS) that are formed under forced stress conditions is a key component of degradation pathway studies performed during late-stage development for monoclonal antibody (mAb) therapeutics. Na...
Automated Enzymatic Deglycosylation of Monoclonal Antibody with the SampleStream Platform [0.03%]
在SampleStream平台上自动化单克隆抗体的酶去糖基化过程
Troy D Fisher,Philip D Compton,Matthew T Robey et al.
Troy D Fisher et al.
Engineered monoclonal antibodies have proven themselves as invaluable biotherapeutics used in clinics worldwide. In discovery, development, and production, it is essential that they are accurately validated to ensure their homogeneity, cons...
Emily Edstrom,Saned Gharari,Eric Davis
Emily Edstrom
Fourier Transform is a low-cost method for improving duty cycle, resolving power, and signal-to-noise ratio in the Ion Mobility Spectrometry (IMS) experiment in a 2-gate IMS cell. By simultaneously pulsing both gates through a frequency swe...
Development and Optimization of an Electrospray Diverter for Reduced Background Signal in Ambient Ionization Sources [0.03%]
一种减少电喷雾分流器背景信号的开发与优化方法研究报告
Seth M Eisenberg,Alena N Joignant,Jonathan D Trumbull et al.
Seth M Eisenberg et al.
A common issue in pulsed ambient ionization sources is the presence of background ions, which can cause ionization suppression and negatively influence downstream statistical analysis. Infrared matrix-assisted laser desorption electrospray ...
Faces of Mass Spectrometry/Fanny Caroline Liu [0.03%]
质谱百家谈/刘凡Carbon-based Microsystems for Point-of-care Diagnostics and Beyond
Anne Brenner,J D Brookbank
Anne Brenner