Adapting an in-house viral gastroenteritis multiplex PCR for use on the Hologic Panther Fusion® system [0.03%]
在Hologic Panther Fusion系统上进行病毒性胃肠炎多重PCR检测的方法学建立和评估
Daphne G J C Mulders,Jolanda J C Voermans,Sander van Boheemen et al.
Daphne G J C Mulders et al.
Background: Viral gastroenteritis is a major global health concern, causing billions of infections annually. Multiplex real-time PCR assays for gastro-intestinal viruses (GIV) ensure timely diagnosis, enabling clinical ma...
Evaluation of One-Step amplicon-based targeted enrichment for SARS-CoV-2 whole-genome sequencing using the Midnight amplicon scheme [0.03%]
利用Midnight扩增子方案评估一步法靶向富集在SARS-CoV-2全基因组测序中的应用价值
Calvin N Ezeanyaegbu,Nikki Pl Toledo,Christopher P Corkum et al.
Calvin N Ezeanyaegbu et al.
Genomic surveillance proved invaluable during the COVID-19 pandemic for tracking SARS-CoV-2 variants and guiding outbreak responses, underscoring the ongoing need to reduce whole-genome sequencing (WGS) costs and improve workflow efficiency...
Evaluation of peptide-based polyclonal antibodies for a lateral flow immunoassay to rapidly detect tomato leaf curl begomoviruses [0.03%]
评估用于快速检测番茄卷曲黄化病毒的侧向流免疫检测试剂中基于肽的多克隆抗体效果
Harun Kimathi Ringeera,Kohei Okuda,Yutaro Neriya et al.
Harun Kimathi Ringeera et al.
Tomato leaf curl diseases caused by begomoviruses result in significant yield losses in solanaceous and cucurbit crops across East Africa. To effectively manage and monitor these diseases, rapid and field-deployable diagnostic tools are ess...
Minghui Zhang,Na Zhang,Xiaonuo Xu et al.
Minghui Zhang et al.
Rabies, a fatal zoonosis, remains a burden in developing countries. Concerns about indirect environmental transmission and appropriate post‑exposure prophylaxis (PEP) are increasing, yet data on RABV stability under various conditions are ...
Performance comparison of rapid and native barcoding methods for Oxford Nanopore sequencing of Poliovirus Viral Protein 1 (VP1) amplicons [0.03%]
牛津纳米孔测序技术快速条形码与原始条形码方法检测脊髓灰质炎病毒蛋白1基因的性能比较分析报告
James E Bullows,Christina J Castro,Caeden Meade et al.
James E Bullows et al.
Accurate and timely sequencing of poliovirus is critical for global eradication efforts, particularly for molecular epidemiology based on the typing region of the genome, viral protein 1 (VP1). While Oxford Nanopore Technologies (ONT) seque...
On-site serological screening of Usutu virus in wild birds: Field evaluation of a lateral flow microarray immunoassay using the WHO REASSURED framework [0.03%]
基于世界卫生组织REASSURED框架的乌苏图病毒现场血清学筛查：野外评估侧流微阵列免疫测定方法
Bijan Godarzi,Cora M Holicki,Felicity D Chandler et al.
Bijan Godarzi et al.
Arboviruses such as Usutu virus (USUV) and West Nile virus (WNV) cycle enzootically between competent mosquito vectors and avian reservoir hosts. With USUV established and WNV incidence rising in the Netherlands, detecting antibodies in wil...
Broadening the use of Virus-Mimicking Artificial Positive Controls in Plant Virus Diagnostics [0.03%]
扩大植物病毒诊断中模仿病毒人工阳性的使用范围
Habeeb Y Atanda,Bolaji Osundahunsi,Shivani Singh et al.
Habeeb Y Atanda et al.
Positive controls are essential for diagnostics yet access to high quality ones remains a persistent bottleneck for assay validation and quality control. Infected tissues are scarce or even regulated, whereas synthetic DNA fragments lack ma...
Epidemiological characteristics of respiratory syncytial virus in children during 2021-2024 [0.03%]
2021-2024年儿童呼吸道合胞病毒感染的流行病学特征分析
Bohao Wang,Jingjing Wang,Zhongyuan Sun et al.
Bohao Wang et al.
Background: Since the autumn of 2023, there has been a notable increase in the incidence of respiratory syncytial virus (RSV) infection among children. This study aimed to demonstrate epidemiology characteristics of RSV d...
Beyond Strain-Specific Immunity: Conserved Antigenic Targets, Emerging Platforms, and Translational Challenges in Universal Influenza and Pan-Coronavirus Vaccine Development [0.03%]
超越特定毒株的免疫：通用流感和泛冠状病毒疫苗开发中的保守抗原靶点、新兴平台及转化挑战
Taruna Ikrar,Wachyudi Muchsin,Alfi Sophian
Taruna Ikrar
Background: The global burden of respiratory viral disease is shaped by two enduring threats: influenza, responsible for 290,000-650,000 annual deaths, and coronaviruses, exemplified by the catastrophic SARS-CoV-2 pandemi...
Analytical Characteristics of the NeuMoDx™ SARS-CoV-2 Assay and Clinical Agreement with the BD MAX System [0.03%]
NeuMoDx™ SARS-CoV-2检测试剂的性能分析及其与BD MAX系统的临床一致性评估
Yi-Hsuan Hsieh,Cheng-Chuan Su,Chu-Che Lee et al.
Yi-Hsuan Hsieh et al.
Accurate and automated molecular detection of SARS-CoV-2 has played an important role in routine clinical diagnostics and laboratory workflow management. This study evaluated the analytical characteristics of the NeuMoDx™ SARS-CoV-2 assay ...