Identification and characterization of a novel α-L-fucosidase from Desertivirga arenae for efficient L-fucose biosynthesis in metabolically engineered Escherichia coli [0.03%]
一株沙漠盐杆菌Desertivirga arenae中新型α-L-岩藻糖苷酶的鉴定、表征及在大肠杆菌中的异源表达纯化改善L-岩藻糖生物合成
Jiawei Meng,Aiguo Li,Zhen Lu et al.
Jiawei Meng et al.
L-Fucose is a physiologically important monosaccharide widely present in various biomass resources, especially microalgae. Metabolically engineered microbial systems have gained growing interest for L-fucose production due to their high yie...
Discovery of a new P450 enzyme DkpdB for cyclo(Trp-Phe) dimerization in the marine strain Aspergillus sydowii MNP-2 [0.03%]
发现并鉴定来自海洋链霉菌MNP-2的环（色氨酸-苯丙胺）二聚化新酶DkpdB
Huawei Zhang,Xiaoying Wang,Yao Ding
Huawei Zhang
Diketopiperazine (DKP) dimers are one of the important microbial natural products owing to their structural diversity and a broad spectrum of biological properties. However, few studies on fungal P450-catalyzed DKP dimerization have been re...
Purification and kinetic characterization of cyclodextrin glycosyltransferase from Alkalihalobacillus Trypoxylicola KRM [0.03%]
来自碱性盐bacillus tryprooxylicola krm的环糊精糖基转移酶的纯化及动力学性质研究
Khushbu Rabadiya,Dimple Pardhi,Khushali Thaker et al.
Khushbu Rabadiya et al.
Cyclodextrin glucanotransferase (CGTase, EC 2.4.1.9) is an enzyme with its unique capability to transform starch and related substrates into cyclodextrins (CDs). Cyclodextrins are widely used in various industries (food, pharmaceuticals, te...
Isolation and identification of a protease-producing bacterium from abalone intestines and its application for degradation of fish proteins [0.03%]
一种产蛋白酶鲍肠道菌的分离鉴定及降解鱼蛋白的应用研究
Seokwon Kang,Junho Yang,Beom-Su Cho et al.
Seokwon Kang et al.
The gastrointestinal tracts of aquatic animals provide a unique environment for microbial growth. Proteases derived from microorganisms in aquatic taxa are particularly valuable owing to their stability under various temperature and salinit...
Systematic modular engineering of genome-integrated Escherichia coli MG1655 for high-level 2'-fucosyllactose production [0.03%]
系统模块化工程整合基因组的大肠杆菌MG1655高效合成2'-岩藻糖基乳糖分子细胞工厂
Roulin Chen,Longhao Yang,Hao Wang et al.
Roulin Chen et al.
2'-Fucosyllactose (2'-FL), the most abundant human milk oligosaccharide (HMO), has attracted considerable interest for its prebiotic and immunomodulatory functions, with broad applications in infant nutrition. In this study, we report the d...
Laiba Hasan,Meryam Sardar
Laiba Hasan
In the present work, silver-based antimicrobial nanocomposites were prepared using alpha-amylase and silver nitrate as precursors. The enzyme acted as both a reducing and stabilizing agent, converting the metal salt into nanoparticles, as v...
Conversion of Poly(3-hydroxybutyrate) (PHB) bioplastics to short-chain fatty acids by acidogenic fermentation: Effect of inoculum to substrate ratio, PHB pre-treatment and operating temperature [0.03%]
多羟基丁酸生物塑料转化为短链脂肪酸的产酸发酵：接种量、聚3-羟基丁酸预处理和操作温度的影响
Chandramouli Mukherjee,Shuyao Wang,Kaushik Venkiteshwaran et al.
Chandramouli Mukherjee et al.
Poly(3-hydroxybutyrate) (PHB) is a biodegradable alternative to petroleum-based plastics, yet its growing production requiring end-of-life management. Acidogenic fermentation offers a promising bio-upcycling route by converting PHB into sho...
Kinetic insights into hydroxyindole oxidation and mediator-free dye decolorization by Trametes zonata 1525 laccase [0.03%]
表没食子酸丙酯动力学研究及无介体降解染料的栓孔菌1525型漆酶的研究
Olha Demkiv,Nataliya Stasyuk,Mariya Horecha et al.
Olha Demkiv et al.
Catalytic activity of fungal laccases toward biologically relevant indole metabolites remains insufficiently explored. In this study, extracellular laccase from Trametes zonata was characterized with particular focus on hydroxyindole oxidat...
Expression and characterization of acetylxylan esterase 2 from Fusarium graminearum [0.03%]
稻瘟菌乙酰木糖酯酶2的表达及鉴定
Michael J Bowman,Kaitlyn N Walls,Todd A Naumann et al.
Michael J Bowman et al.
Xylan, one of the sugar polymer components of biomass, is complex in the number and types of glycosidic bonds that comprise its structure. Due to this complexity, xylan hydrolysis requires many different enzymatic activities to fully releas...
Engineering AaADH1 for enhanced catalytic efficiency and solubility via substrate tunnel modulation [0.03%]
通过底物隧道调节工程化AaADH1以提高催化效率和溶解度
Chenyu Wang,Xiao Feng,Lixia Zong et al.
Chenyu Wang et al.
Artemisinic alcohol dehydrogenase 1 (AaADH1) plays a pivotal role in the biosynthesis of artemisinic acid, a key precursor of the antimalarial drug artemisinin. However, its low catalytic activity and poor yield limit its industrial applica...