Harnessing in vitro platforms for natural product research: in vitro driven rational engineering and mining (iDREAM) [0.03%]
利用体外平台进行天然产物研究：体外驱动的合理工程和挖掘(iDREAM)
Ran Liu,Dingchen Yu,Zixin Deng et al.
Ran Liu et al.
Well-known issues amid in vivo research of natural product discovery and overproduction, such as unculturable or unmanipulable microorganisms, labor-intensive experimental cycles, and hidden rate-limiting steps, have hampered relevant inves...
Zhiliang Yang,Mark Blenner
Zhiliang Yang
Yeasts are used to produce a myriad of value-added compounds. Engineering yeasts into cost-efficient cell factories is greatly facilitated by the availability of genome editing tools. While traditional engineering techniques such as homolog...
Lyndsay Stapleton,Yuanjia Zhu,Yi-Ping Joseph Woo et al.
Lyndsay Stapleton et al.
Ischemic heart disease is the most common type of heart disease, responsible for roughly 10 million deaths worldwide annually. While standard clinical interventions have resulted in improved patient outcomes, access to small diameter vessel...
Xuexiang Zhang,Tae-Hyung Kim,Timothy J Thauland et al.
Xuexiang Zhang et al.
Immune cells can sense and respond to biophysical cues - from dynamic forces to spatial features - during their development, activation, differentiation and expansion. These biophysical signals regulate a variety of immune cell functions su...
Mitigation of host cell mutations and regime shift during microbial fermentation: a perspective from flux memory [0.03%]
微生物发酵过程中宿主细胞突变及状态转换的缓解措施：从代谢流记忆的角度看
Jeffrey J Czajka,Burak Okumuş,Mattheos Ag Koffas et al.
Jeffrey J Czajka et al.
Microbial engineering forces flux redistribution to accommodate higher production rates, straining the cellular supply chain and leading to growth deficiency. Thus, there is a selective pressure to alleviate metabolic burden and revert towa...
Phage-based target discovery and its exploitation towards novel antibacterial molecules [0.03%]
基于噬菌体的靶点发现及其对新型抗菌分子的应用探索
Xing Wan,Hanne Hendrix,Mikael Skurnik et al.
Xing Wan et al.
The deeply intertwined evolutionary history between bacteriophages and bacteria has endowed phages with highly specific mechanisms to hijack bacterial cell metabolism for their propagation. Here, we present a comprehensive, phage-driven str...
Circadian rhythms and meal timing: impact on energy balance and body weight [0.03%]
生物钟与用餐时间：对能量平衡及体重的影响
Hedda L Boege,Mehreen Z Bhatti,Marie-Pierre St-Onge
Hedda L Boege
Energy metabolism and appetite regulating hormones follow circadian rhythms which, when disrupted, could lead to adverse metabolic consequences. Such circadian misalignment, a mismatch between endogenous circadian rhythms and behavior, is m...
Engineering natural and noncanonical nicotinamide cofactor-dependent enzymes: design principles and technology development [0.03%]
工程化改造天然和非常见尼克酰胺辅酶依赖的酶：设计原理与技术发展
Edward King,Sarah Maxel,Han Li
Edward King
Nicotinamide cofactors enable oxidoreductases to catalyze a myriad of important reactions in biomanufacturing. Decades of research has focused on optimizing enzymes which utilize natural nicotinamide cofactors, namely nicotinamide adenine d...
Rui Chen,Ji Jing,Stefan Siwko et al.
Rui Chen et al.
Synthetic biology, when combined with immunoengineering (designated synthetic immunology), has enabled the invention of an arsenal of genetically encoded synthetic devices and systems to reprogram cells for therapeutic purposes. The enginee...
Optogenetics and biosensors set the stage for metabolic cybergenetics [0.03%]
光遗传学和生物传感器为代谢网络的细胞工程奠定基础
César Carrasco-López,Sergio A García-Echauri,Therese Kichuk et al.
César Carrasco-López et al.
Cybergenetic systems use computer interfaces to enable feed-back controls over biological processes in real time. The complex and dynamic nature of cellular metabolism makes cybergenetics attractive for controlling engineered metabolic path...