Resource allocation models: theory and applications in microbial biotechnology [0.03%]
资源分配模型：理论与微生物技术中的应用
Bas Teusink,Pranas Grigaitis,Maaike Remeijer et al.
Bas Teusink et al.
Wild-type cells allocate their limited resources to express proteins that support growth and survival in their natural environments. In contrast, biotechnology aims to maximize key performance indicators such as yield, productivity, or tite...
Lena M Hümmler,Stefan Hristov,Lucas Hille et al.
Lena M Hümmler et al.
Growth-coupled bioproduction (GCBP) is a metabolic engineering approach that introduces an obligatory dependency between metabolic activity and production, ensuring a high minimal yield and genetic stability. Here, product yield is primaril...
Microfluidics meets cell-free systems: from molecular engineering to synthetic cells [0.03%]
微流体与无细胞系统：从分子工程到合成细胞
Amogh K Baranwal,Sebastian J Maerkl
Amogh K Baranwal
Cell-free systems have emerged as a powerful platform for protein production, characterization, and bottom-up construction of artificial cells, offering direct control over biochemical environments. However, achieving high-throughput and it...
Jiho Seok,Mark P Styczynski
Jiho Seok
Metabolic engineering employs microbial cell factories to produce high-value products from low-cost feedstocks. Designing, optimizing, and evaluating biosynthetic pathways in microbial cell factories is essential, yet these processes remain...
Engineering metabolism of Saccharomyces cerevisiae for production of chemicals [0.03%]
利用工程酵母代谢生产化学品
Yi Yu,Xiaoying Fu,Jinmiao Hu et al.
Yi Yu et al.
The sustainable production of chemicals from renewable, nonedible biomass has become crucial to address environmental challenges like climate change and resource depletion caused by fossil resource dependence. Saccharomyces cerevisiae has e...
Kate Collins,Claire E Stanley,Thomas E Ouldridge
Kate Collins
The capacity to pattern biomolecules within microfluidic devices expands the scope of microfluidic technologies. In such patterned systems, surface-bound components remain localized, while the microfluidic network supplies reagents and remo...
Controlled protein synthesis and spatial organisation in microfluidic environments [0.03%]
微流体环境下的蛋白质控制合成及空间组织
Aukse Gaizauskaite,Emma E Crean,Imre Banlaki et al.
Aukse Gaizauskaite et al.
Performing cell-free expression (CFE) in tailored microfluidic environments is a powerful tool to investigate the organisation of biosystems from molecular to multicellular scales. While cell-free transcription-translation systems simplify ...
Building trust in automated experimentation: uncertainty quantification in the era of high-throughput biolabs [0.03%]
自动化实验中的信任构建：高通量生物实验室时代的不确定性量化
Wolfgang Wiechert,Laura M Helleckes,Katharina Nöh
Wolfgang Wiechert
Uncertainty quantification (UQ) is central to data analytics, particularly in the life sciences, where experiments are often affected by significant measurement noise. In emerging automated high-throughput biolabs, such as biofoundries, par...
Ionizable lipids empowered with artificial intelligence for mRNA delivery [0.03%]
基于人工智能的离子型脂质用于mRNA药物传输
Gang Zhang,Zongchao Zuo,Jingzhi Wang et al.
Gang Zhang et al.
Ionizable lipids are the most significant component of lipid nanoparticles and govern the ability to deliver mRNA in vitro and in vivo. Traditional development methods for ionizable lipids depend on the labor-intensive multistep synthesis a...
Felix M Hubert,Anna C Love,Tian Lan et al.
Felix M Hubert et al.
Cannabis sativa and related plants are recognized for their psychoactive and therapeutic effects due to the unique molecules they produce, including phytocannabinoids. Beyond the major phytocannabinoids Δ⁹-tetrahydrocannabinol and cannabi...