Imaging genome dynamics in real time with CRISPR-based technologies [0.03%]
基于CRISPR的技术在真核生物中实现基因组动态的实时成像
Benjamin G Adams,Jiahui Wu
Benjamin G Adams
Gene expression is a fundamental aspect of cellular function, driving diverse biological processes and disease. Dynamic interactions between genomic loci play an essential role in gene regulation. Therefore, visualizing the spatiotemporal d...
Proximity chemistries to study RNA biology at the subcellular scale [0.03%]
用于在亚细胞水平上研究RNA生物学的邻近标记化学方法
Rachel Shu Ting Chan,Kanokpol Aphicho,Bryan C Dickinson
Rachel Shu Ting Chan
RNAs preferentially localize across virtually every subcellular compartment, from membrane-bound organelles, membrane-less condensates and even the cell surface, where they perform locale-specific functions in close coordination with RNA-bi...
Discovery and engineering of polymerases and ligases for the synthesis of modified nucleic acids [0.03%]
用于合成修饰核苷酸的聚合酶和连接酶的发现与工程改造
Sumire Honda Malca,Peter Stockinger,Miquel Estévez-Gay et al.
Sumire Honda Malca et al.
Oligonucleotides have emerged as a rapidly expanding modality in therapeutics, diagnostics, and synthetic biology, increasing manufacturing demand. However, solid phase oligonucleotide synthesis, the dominant production technology, is hampe...
Therapeutic potential and resistance of proline-rich antimicrobial peptides [0.03%]
富含脯氨酸的抗菌肽的治疗潜力和抗性
Chaoran Liang,Tianmeng Zhang,Aydin Menekse et al.
Chaoran Liang et al.
The rapid emergence of multidrug-resistant bacteria has underscored the urgent need for alternative antimicrobial strategies. Proline-rich antimicrobial peptides (PrAMPs) constitute a unique class of antimicrobial peptides characterised by ...
Always on the move: Mapping spatiotemporal interactome with proximity labeling in mammalian cells [0.03%]
时空互动组学映射：哺乳动物细胞中利用邻近标记进行蛋白质相互作用动力学研究
Chun Hei Li,Ginny G Farías
Chun Hei Li
Compartmentalization is central to coordinate complex biochemical processes in eukaryotic cells. Proteins governing organelle function and proteome complexity are dynamically trafficked intracellularly and intercellularly to maintain cellul...
Light-driven spatial proteomics: Photocatalytic strategies for mapping protein microenvironments [0.03%]
光驱动的空间蛋白质组学：用于绘制蛋白质微环境的光电催化策略
Neha Somalwar,Xiaolin Nan,Andrew Emili
Neha Somalwar
Modern proteomic efforts aim to define protein composition and protein-protein interactions within native spatial tissue contexts to understand disease mechanisms and therapeutic responses. Two complementary strategies dominate this effort:...
Recent development of fluorescent probes for hydrogen per/polysulfides [0.03%]
近年来荧光探针检测过氧化氢和多硫化物的研究进展
Meg Shieh,Zhengyuan Jiang,John Y Qiu et al.
Meg Shieh et al.
While there is a burgeoning understanding of the overall importance of hydrogen per/polysulfides (H2Sn, n ≥ 2) in human health, their specific biological roles and mechanisms of action remain unclear. To better understand these critical de...
Medical isotope-labeled nucleic acids: A decade of progress toward theranostics (2015-2025) [0.03%]
医用放射性核素标记核酸：向诊疗一体化迈进的十年（2015-2025）
Antonio A W L Wong,François Bénard,David M Perrin
Antonio A W L Wong
Medical isotope-labeled nucleic acids represent an emerging theranostic platform at the intersection of radiochemistry and oligonucleotide therapeutics. This review surveys studies published between 2015 and 2025, examining radiochemistry f...
Indigoidine synthetases: Versatile biocatalysts in nature and the laboratory [0.03%]
吲哚酮生物合成酶：自然和实验室中的多功能生物催化剂
Alistair S Brown,David F Ackerley
Alistair S Brown
Indigoidine synthetases are unique single-module non-ribosomal peptide synthetases (NRPSs) that can act autonomously to create a pigmented product, the vivid blue 3,3'-bipyridyl compound indigoidine. Recent techno-economic and life cycle an...
Genetic code expansion: Bridging synthetic biology precision and cellular complexity to decipher molecular mechanisms of diseases [0.03%]
扩展遗传密码：融合合成生物学的精确性与细胞的复杂性以解析疾病的分子机制
Wanchana Phromkrasae,Michele Bertacchi,Michèle Studer et al.
Wanchana Phromkrasae et al.
Despite major advances in biomedical research, dissecting disease-relevant molecular pathways remains challenging due to pathway redundancy, transient protein interactions, and the limited spatiotemporal precision of existing tools. Genetic...