Cool and collected: Advances in sample preparation for cryo-electron microscopy [0.03%]
cool and collected：低温电子显微学样品制备技术的进展
Shani Tcherner Elad,Noa Ben-Asher,Leeya Engel
Shani Tcherner Elad
Cryo-electron microscopy (cryo-EM) has emerged as a transformative tool in structural biology, enabling high-resolution visualization of macromolecules in their native states. Cryo-focused ion beam milling (cryo-FIB) and other advances in s...
Francesca M Marassi,Guido Pintacuda
Francesca M Marassi
Membrane proteins have evolved to function as part of specialized biological membranes, and their structures and activities are highly susceptible to their local environment. Detergents and lipid mimetics replicate certain aspects of biolog...
Weihua Qiu,Youzhong Guo
Weihua Qiu
The native cell membrane nanoparticles (NCMN) system utilizes membrane-active polymers specifically designed and optimized to extract and stabilize membrane proteins in the form of NCMN particlesfor biochemical and biophysical characterizat...
Tanaya Basu Roy,Mana Heidari,Nikolay V Dokholyan
Tanaya Basu Roy
Optogenetically regulated enzymes offer unprecedented spatiotemporal control over protein activity, intermolecular interactions, and intracellular signaling. Many design strategies have been developed for their fabrication based on the prin...
HIV-1 gp160 in nanodiscs: Unravelling structures and guiding vaccine design [0.03%]
纳米盘中的HIV-1gp160：揭示结构并指导疫苗设计
Nancy M Elbaz,Mahmoud L Nasr
Nancy M Elbaz
The stabilization of HIV-1 gp160 trimers (Env) within phospholipid bilayer nanodiscs has provided critical structural insights into the membrane-proximal external region (MPER) and the broader dynamics of gp160. Cryo-EM and molecular simula...
R Gonzalo Parra,Diego U Ferreiro
R Gonzalo Parra
The controlled dissipation of chemical potentials is the fundamental way cells make a living. Enzyme-mediated catalysis allows the various transformations to proceed at biologically relevant rates with remarkable precision and efficiency. T...
Orchestrating function: Concerted dynamics, allostery, and catalysis in protein tyrosine phosphatases [0.03%]
协调的功能：蛋白质酪氨酸磷酸酶的协同动力学、别构和催化作用
Virgil A Woods,Shivani Sharma,Alexis M Lemberikman et al.
Virgil A Woods et al.
Protein tyrosine phosphatases (PTPs) are a family of enzymes that play critical roles in intracellular signaling and regulation. PTPs are conformationally dynamic, exhibiting motions of catalytic loops and additional regions of the structur...
Emerging paradigms in the lateral and transverse organization in biological membrane and their functional implications: Connecting the dots with biomolecular simulations [0.03%]
生物膜横向和侧向组织的新范式及其功能意义：与生物分子模拟相结合的研究进展
Anand Srivastava
Anand Srivastava
Since the publication of the first papers in the early 1990s, molecular simulation as a reliable biophysical tool in the area of membrane biophysics has come a long way. Advances in simulation algorithms, coupled with exascale computing hav...
Yuan He,Yawen Bai
Yuan He
Approaches for regulating enzyme activities: Recent advances in experiment and computation [0.03%]
调节酶活性的手段：实验与计算研究新进展
Qiang Cui
Qiang Cui
Major progress has been made in recent years in terms of strategies for regulating enzyme activities. Novel high-throughput enzyme kinetic assays and efficient computational methodologies enabled a deeper understanding of molecular mechanis...