Sanjeev Ghimire,Sterling L N Nicholas,Hugo Sanabria
Sanjeev Ghimire
Biomolecules' conformational landscapes span multiple timescales and structural populations, challenging traditional structural biology methods that predominantly capture static states. Over the past three decades, Förster resonance energy...
Shared parts, different structures: How asymmetry drives IgM and IgA structure-function relationships [0.03%]
共用部分，不同结构：不对称性如何推动IgM和IgA的结构功能关系
Rebecca M Schneider,Yujia Ji,Beth M Stadtmueller
Rebecca M Schneider
Polymeric (p) immunoglobulins (Igs) and their secretory (S) forms are critical to vertebrate immunity. The pIgs and SIgs comprise a molecularly diverse family of antibodies that contain multiple Ig monomers, up to one joining chain (JC), an...
Single-condensate fluorescence approaches: Resolving heterogeneity in molecular organization, dynamics and material state [0.03%]
单凝聚荧光方法：分子组织、动力学和材料状态异质性的解析
David Polanco,Blanca Viguri-Lamata,Thomas S van Zanten et al.
David Polanco et al.
Biomolecular condensates organize the intracellular space by linking molecular interactions to mesoscale properties. While ensemble-averaged measurements provide insights into phase behavior, they obscure the intrinsic heterogeneity and asy...
Probing the complexity of cellular RNA folding: Ensembles, intermediates, and isoforms [0.03%]
探索单细胞RNA折叠的复杂性：聚集体、中间体和等位基因
Anthony M Mustoe,Danny Incarnato
Anthony M Mustoe
RNA molecules populate complex structural landscapes that are continuously reshaped throughout the RNA lifecycle by equilibrium and non-equilibrium processes. Resolving these structural landscapes represents a central challenge in RNA bioch...
Advancing bacterial cell biology with in-cell cryo-electron microscopy [0.03%]
利用细胞内冷冻电子显微镜推动细菌细胞生物学的发展
Rui Liu,Margot Riggi,Kit Pogliano et al.
Rui Liu et al.
Over the past decades, bacterial cell biology has revealed that bacteria possess complex subcellular structures and regulatory mechanisms once thought to be unique to eukaryotes, establishing them as powerful model systems for studying fund...
Integrative structural biology of protein-RNA interactions in four dimensions [0.03%]
四维蛋白质-核糖核酸相互作用的整合结构生物学
Simone Heber,Janosch Hennig
Simone Heber
Protein-RNA complexes underlie essential cellular processes and understanding their functional mechanisms requires structural analysis. Yet, their inherent flexibility, multi-valency, and dynamics make them challenging targets for structura...
Crowding effects on biomolecular processes: Computational advances and challenges [0.03%]
分子聚集效应对生物分子过程的影响：计算方法的进展和挑战
Shivnandi,Divya Nayar
Shivnandi
Macromolecular crowding is a fundamental feature of the cellular environment that strongly influences biomolecular structure, dynamics, and phase behavior. Despite its significance, it has been a challenge to represent and capture the effec...
Recent advances on structure and dynamics of G protein-coupled receptors using single-molecule Förster resonance energy transfer [0.03%]
单分子福斯特共振能量转移在研究G蛋白偶联受体结构和动力学方面的最新进展
Robert B Quast,Emmanuel Margeat
Robert B Quast
G protein-coupled receptors (GPCRs) mediate cellular responses to a wide array of extracellular stimuli, including photons, ions, small molecules, peptides, and proteins, orchestrating nearly all physiological processes. This superfamily is...
Mapping the relationship between receptor dynamics and function in G protein-coupled receptors using molecular dynamics [0.03%]
利用分子动力学研究G蛋白偶联受体中受体动态与功能之间的关系地图
Adrián García-Recio,Alejandro Peralta-García,Anna Korda et al.
Adrián García-Recio et al.
G protein-coupled receptors (GPCRs) are key regulators of cellular signaling and major drug targets. Although X-ray crystallography and cryo-electron microscopy have provided high-resolution receptor structures, these static snapshots captu...
Detergent-free extraction polymers in membrane protein structure determination [0.03%]
无洗涤剂萃取聚合物在膜蛋白结构测定中的应用
Naomi L Pollock,Stephen P Muench,Alice J Rothnie et al.
Naomi L Pollock et al.
Membrane proteins (MPs) underpin essential cellular processes, representing ∼60% of drug targets, but their structural characterisation remains challenging with bottlenecks across production, solubilisation, stabilisation and biophysical a...