Transcription factors as drivers of 3D enhancer-promoter interactions [0.03%]
转录因子作为三维基因组构象下增强子与启动子相互作用的驱动者
Nicholas G Aboreden,Gerd A Blobel
Nicholas G Aboreden
The molecular basis for specific pairing between transcriptional enhancers and promoters remains a highly active area of investigation, even over four decades after enhancers were first discovered. Numerous studies have explored general mec...
Ribonucleic acid as an active driver of protein aggregation in neurodegeneration [0.03%]
RNA作为蛋白质聚集在神经退行性疾病中的一种活性驱动因素
Francesco A Aprile,Annalisa Pastore
Francesco A Aprile
Neurodegeneration has traditionally been largely attributed to protein aggregation, yet ribonucleic acid (RNA) has emerged as an active driver of pathology. Expanded repeat RNAs, misregulated RNA-binding proteins, and aberrant RNA-protein i...
Heterogeneity of glycoproteins: Why does it matter and how to account for it [0.03%]
糖蛋白的异质性：为什么重要以及如何解决该问题
Elisa Fadda,Ojas Singh,Benjamin L Schulz
Elisa Fadda
Glycosylation can be critical for determining the structure and functions of proteins, but it is often neglected, leading to significant knowledge gaps in our understanding of biology. The inherent heterogeneity of glycans presents technica...
Kasper Andreasen Clowes,Camilla Gottlieb Andersen,Adriana Chrenková et al.
Kasper Andreasen Clowes et al.
Sugar transporters are essential for the long-distance carbon allocation in plants and contribute to both biotic and abiotic stress responses yet the molecular mechanisms of substrate specificity and conformational cycling are only beginnin...
High-speed atomic force microscopy of membrane and membrane protein dynamics [0.03%]
膜和膜蛋白的高速原子力显微镜观测技术
Abeer Alshammari,Bashra Mahamed,George R Heath
Abeer Alshammari
High-speed atomic force microscopy (HS-AFM) enables direct nanometer-resolution visualization of single molecules and molecular assemblies in real-time and under physiological conditions, providing unique insights into how membranes and mem...
Context matters: A view on metadata analysis in cryo-electron tomography [0.03%]
背景很重要：低温电子层析成像中的元数据分析观点
Markus Schreiber,Beata Turoňová
Markus Schreiber
Cryo-electron tomography (cryo-ET) enables the analysis of biological samples in situ, revealing the complex interplay that shapes (sub)cellular architecture. Across the cryo-ET workflow, diverse forms of contextual information-from experim...
Labelling in cryogenic electron tomography - Bridging the gap between correlative light and electron microscopy and protein identification [0.03%]
用于低温电子断层扫描的标记技术——相关光镜与电镜和蛋白质识别之间的桥梁技术
Emily A Machala,Lindsay A Baker
Emily A Machala
Cryogenic electron tomography (cryoET) offers unparalleled views into the molecular architecture of cells. As no stains or fixation are used, electrons scatter off the native atoms, and all molecules contribute to the final tomogram. As a r...
From filtering to denoising: Increasing visual interpretability of cryo-electron tomograms [0.03%]
从滤波到降噪：提高冷冻电子断层扫描图像的视觉解释性
Théophile Stoll,Florian Fäßler
Théophile Stoll
Cryo-electron tomography has emerged as the premier technique for ultrastructural analysis of natively preserved biological specimens and in situ structure determination. Each tomogram of a cell contains valuable information on the imaged m...
Physical genomics: Why gene regulation is tug of war between polymer physics and biochemistry [0.03%]
物理基因组学：为什么基因调控是聚合物物理与生物化学之间的博弈
Sarah Harris,Agnes Noy,Wilma K Olson
Sarah Harris
DNA-protein interactions underlie genome activity, governing gene expression as well as the physical organisation of DNA. Until recently, DNA-protein complexes were predominantly described at atomic resolution using short DNA fragments, con...
From memorization to generalization: Why physics will improve machine learning -based prediction of protein complexes [0.03%]
从记忆到泛化：为什么物理会改善基于机器学习的蛋白质复合物预测
Ernest Glukhov,Sandor Vajda,Dima Kozakov
Ernest Glukhov
AlphaFold-like models have transformed monomer structure prediction yet reliable, generalizable prediction of protein-protein interactions (PPIs) remains challenging, particularly for antibody-antigen docking. These limitations often stem f...