Marcela de Barros,Gregory Labrie,Carla Mattos
Marcela de Barros
Our previously proposed Ras dimerization model is consistent with recent details observed by NMR in that Raf activation is centered on the Ras/Raf dimer, distinct from one in which Ras activates Raf as a monomer with the Raf cysteine rich d...
Ruth Nussinov,Hyunbum Jang
Ruth Nussinov
Drug residence time defines the duration the drug is bound to its protein target. It is a crucial determinant of drug action. Yet, a priori estimating it in the design could be the most challenging. The mechanisms of allosteric and orthoste...
Recent advances in quantifying protein conformational ensembles with dipolar EPR spectroscopy [0.03%]
利用双极EPR光谱量化蛋白质构象集合的最新进展
Reza Dastvan,Stefan Stoll
Reza Dastvan
This perspective highlights recent applications and technological progress in dipolar electron paramagnetic resonance (EPR) spectroscopy, including double electron-electron resonance (DEER) spectroscopy. These methods provide nanoscale dist...
Cryo-focused ion beam milling for cryo-electron tomography: Shaping the future of in situ structural biology [0.03%]
低温聚焦离子束研磨技术在低温电子断层扫描中的应用：现场结构生物学的未来形状
Sven Klumpe,Jürgen M Plitzko
Sven Klumpe
Cryo-focused ion beam instruments to produce cellular thin sections for subsequent imaging by cryo-electron tomography have become an integral part of the methodologies for in situ structural biology, enabling high-resolution imaging of bio...
Rafal Czapiewski,Nick Gilbert
Rafal Czapiewski
In mammalian cells, RNA species make up ∼10% of chromatin by mass and play a structural role in the nucleus by acting as scaffolds and influencing genome organisation. Although many proteins bind nuclear RNAs, these interactions are often ...
Rui João Loureiro,Satyabrata Maiti,Kuntal Mondal et al.
Rui João Loureiro et al.
RNA and RNA-protein (RNP) complexes are central to many cellular processes, but the determination of their structures remains challenging due to RNA flexibility and interaction diversity. This review highlights recent computational advances...
Functional sub-states link conformational landscapes and protein evolution [0.03%]
功能亚稳态连接构象景观与蛋白质进化
Morito Sakuma,Karol Buda,H Adrian Bunzel et al.
Morito Sakuma et al.
The intrinsic conformational flexibility of proteins creates structural heterogeneity, giving rise to conformational ensembles within the energy landscape. When conformational ensembles harbor distinct functional sub-states, mutations can r...
Advances in Protein-RNA aptamer recognition and modeling: Current trends and future perspectives [0.03%]
蛋白质-RNA适配体识别与建模研究进展：现状、趋势及展望
Liming Qiu,Xiaoqin Zou
Liming Qiu
RNA aptamers possess a remarkable ability to selectively target a diverse spectrum of biomolecules with exceptional affinity and specificity. Their distinctive physical and chemical attributes have driven extensive research into their thera...
Generative AI techniques for conformational diversity and evolutionary adaptation of proteins [0.03%]
生成AI技术在蛋白质构象多样性和进化适应性方面的应用
Alfie-Louise R Brownless,Dariia Yehorova,Colin L Welsh et al.
Alfie-Louise R Brownless et al.
The advent of AlphaFold and consumer large language models have elicited unprecedented development of artificial intelligence (AI). AI has had substantial impact in every area of research, including in molecular biology. This is principally...
Burcu Aykac Fas,Zeynep Erge Akbas Buz,Turkan Haliloglu
Burcu Aykac Fas
Enzymes are inherently dynamic entities, with their functions intricately governed by the interplay between conformational dynamics - ranging from local residue fluctuations to global motions - and biochemical activity. Deciphering how such...