Exploiting a rational β-strand insertion strategy and disulfide locking to mechanically manipulate domain-swapped protein structures [0.03%]
利用理性的β-折叠插入策略和二硫键锁定机械操纵互换结构域蛋白质结构
Alireza Ghanbarpour,Nikolas Kenaya,Courtney Bingham et al.
Alireza Ghanbarpour et al.
In domain swapping, the "hinge loop" region of the domain-swapped (DS) structure that connects two structurally arbitrary domains undergoes a large structural alteration. While previous studies have shed light on the role of the hinge regio...
A structure-guided approach to predict MHC-I restriction of T cell receptors for public antigens [0.03%]
基于结构预测公共抗原TCR-MHCI限制的思路
Sagar Gupta,Nikolaos G Sgourakis
Sagar Gupta
Peptides presented by major histocompatibility complex class I (MHC-I) proteins provide biomarkers for therapeutic targeting using T cell receptors (TCRs), TCR-mimicking antibodies (TMAs), or other engineered protein binders. Despite the ex...
SeaMoon: From protein language models to continuous structural heterogeneity [0.03%]
SeaMoon：从蛋白质语言模型到连续结构异质性
Valentin Lombard,Dan Timsit,Sergei Grudinin et al.
Valentin Lombard et al.
How proteins move and deform determines their interactions with the environment and is thus of the utmost importance for cellular functioning. Following the revolution in single protein 3D structure prediction, researchers have focused on r...
Geometry-aware template matching for cryo-electron tomograms in Dynamo [0.03%]
用于Dynamo中低温电子层析扫描的几何感知模板匹配方法
Raffaele Coray,Daniel Castaño-Díez
Raffaele Coray
Template matching has a long history of serving as a tool for the automated analysis of volumes in cryo-electron tomography (cryo-ET). Recent theoretical and computational studies of the technique have pinpointed the importance of using fin...
Mingtao Huang,Jinying Ma,Xiaoyu Fu et al.
Mingtao Huang et al.
Helical structure is fundamental for filamentous and tubular macromolecular assemblies that play crucial roles in structural scaffolding and signaling processes. Structure determination of these assemblies relies on the precise estimation o...
CryoEM-enabled visual proteomics reveals de novo structures of oligomeric protein complexes [0.03%]
基于 cryoem 的视觉蛋白质组学可揭示寡聚蛋白复合物的从头结构
Yuanbo Shen,Ailiena O Maggiolo,Tianzheng Zhang et al.
Yuanbo Shen et al.
Single particle cryoelectron microscopy (cryoEM) and cryoelectron tomography (cryoET) are powerful methods for unveiling unique and functionally relevant structural states. Aided by mass spectrometry and machine learning, they promise to fa...
Structural basis for no retinal binding in flotillin-associated rhodopsins [0.03%]
无视紫红质结合的floppin相关视紫红质结构基础
Kirill Kovalev,Artem Stetsenko,Florian Trunk et al.
Kirill Kovalev et al.
Rhodopsins are light-sensitive membrane proteins capturing solar energy via a retinal cofactor covalently attached to a lysine residue. Several groups of rhodopsins lack the conserved lysine and showed no retinal binding. Recently, flotilli...
Structures of USP7 in active and inactive states bound to DNMT1 revealed by cryo-EM [0.03%]
通过冷冻电子显微镜解析的USP7结合DNMT1处于激活和失活状态下的结构
Nao Nakamura,Sae Yoshimi,Amika Kikuchi et al.
Nao Nakamura et al.
The ubiquitin signal generated by UHRF1 is essential for DNA methylation maintenance by recruiting DNA methyltransferase 1 (DNMT1) to hemimethylated DNA through strong binding of its replication foci targeting sequence (RFTS) domain to ubiq...
Structure of a type II SMC Wadjet complex from Neobacillus vireti [0.03%]
来自Neobacillus vireti的II型SMC Wadjet复合体的结构
Florian Roisné-Hamelin,Hon Wing Liu,Stephan Gruber
Florian Roisné-Hamelin
Structural maintenance of chromosome complexes are essential DNA-folding motors that facilitate critical cellular functions, including chromosome segregation and DNA repair. Wadjet systems are prokaryotic SMC complexes specialized in cellul...
Elucidating PTEN conformational dynamics and phosphatase regulation via integrative modeling and mutation prediction [0.03%]
整合建模和突变预测阐明PTEN构象动力学及磷酸酶调控作用
Jennifer Erin Dawson,Iris Nira Smith,Ann Marie Tushar et al.
Jennifer Erin Dawson et al.
PTEN (Phosphatase and TENsin homolog deleted on chromosome ten) is a major tumor suppressor gene that is frequently mutated or lost under cancerous conditions. PTEN is a dual-specificity phosphatase that negatively regulates the PI3K/AKT/mT...