Structural, functional, and mechanistic studies of the bacterial divisome FtsWIQBL in complex with antibiotics [0.03%]
与抗生素结合的细菌分裂复合物FtsWIQBL的结构、功能和机制研究
Shimin Zhu,Yanjie Hu,Rong Wang et al.
Shimin Zhu et al.
Septal peptidoglycan (sPG) biosynthesis during bacterial cell division is driven by the dynamic divisome complex. Its core components, glycosyltransferase FtsW and transpeptidase FtsI are responsible for glycan chain polymerization and cros...
Qinzhe Wang,Enrique Balderas,Alejandro Jara Ramos et al.
Qinzhe Wang et al.
Polyamines, well-known regulators of the mitochondrial calcium (Ca2+) uniporter channel, show unexpected effects when binding the channel from within the matrix. Using cryo-EM, molecular dynamics simulations, and mutagenesis experiments, we...
Streamlined montage cryo-electron tomography for exploring the ultrastructure of cells and tissues [0.03%]
一种用于探索细胞和组织超结构的蒙片低温断层扫描方法
Ryan Hylton,Micaela Boiero Sanders,Adriana Prajica et al.
Ryan Hylton et al.
Cryo-electron tomography reveals the architecture of biological specimens at molecular resolution, albeit with a limited field of view. Montage tomography overcomes this limitation, but existing strategies depend on complex dose distributio...
Structural insight into the role of the periplasmic chaperone Skp in pathogenic Neisseria [0.03%]
.SKp分子伴侣的结构研究及其在致病性奈瑟氏球菌中的作用
Shubham Dubey,Julie Stoudenmire,Pooneh Tavakoley Gheinani et al.
Shubham Dubey et al.
Periplasmic chaperones prevent misfolding and aggregation of proteins in the periplasm and those destined for the outer membranes of Gram-negative bacteria. SurA and Skp are two major periplasmic chaperones, with Skp being the most critical...
Structural basis for oligoclonal T cell recognition of a shared NRAS cancer neoantigen [0.03%]
寡克隆T细胞识别共享NRAS癌症新抗原的结构基础
Vijay Kumar Sharma,D Travis Gallagher,Shayana Saravanakumar et al.
Vijay Kumar Sharma et al.
T cell receptors (TCRs) specific for cancer neoantigens are important for anti-tumor immunity and immunotherapy. To understand the structural basis for T cell recognition of cancer neoantigens, we studied oligoclonal TCRs from patients with...
Cryo-EM structures of human caspase-4 in complex with full-length gasdermin D [0.03%]
与全长Gasdermin D结合的人类Caspase-4的 cryo-EM结构
Ying Luan,Enbo Chen,Youdong Mao
Ying Luan
Caspase-4 drives non-canonical inflammasome signaling by cleaving gasdermin D (GSDMD) to trigger pyroptosis. Cleavage of the interdomain linker (IDL) in caspase-4 yields distinct autoprocessed forms-p20/p12, p22/p10, and p20/p10. While both...
Structures of the Xcp and Hxc T2SS endopili provide new insights into type IV pili subfamilies [0.03%]
Xcp和Hxc T2SS内鞭毛的结构为IV型菌毛亚家族提供了新的见解
Ravi R Sonani,Iman Chouikha,Geneviève Ball et al.
Ravi R Sonani et al.
Type IV filaments (T4F) are widespread bacterial appendages involved in adhesion, motility, DNA uptake, and protein secretion. In the type II secretion system (T2SS), a short pilus-like structure termed the endopilus drives protein export a...
Structure of Perinereis linea erythrocruorin reveals a compact extracellular globin megacomplex [0.03%]
线性环节螠血红蛋白的结构揭示了一种紧凑的细胞外球蛋白巨复合物
Jia-Xin Deng,Wen-Bin Cheng,Kang Xu et al.
Jia-Xin Deng et al.
Many invertebrates lack erythrocytes and instead rely on extracellular hemoglobin assemblies, termed erythrocruorins, for oxygen transport. Here we report a 2.84 Å cryo-electron microscopy (cryo-EM) structure of Perinereis linea erythrocru...
Stephen Brohawn
Stephen Brohawn
In this meet-the-author Q&A, we speak to Stephen Brohawn from the University of California, Berkeley, about his research group's recent Structure paper entitled "Structural basis for proton inhibition of the two-pore domain K+ channel TASK-...
Tetraspanins bring Norrin into focus: Structural insights into ligand-specific Wnt signaling [0.03%]
四跨膜蛋白使Norrin聚焦：配体特异性Wnt信号通路的结构见解
Xiaofeng Qi
Xiaofeng Qi
In this issue of Structure, Xue et al.1 present the cryo-EM structure of the Norrin-Tspan12 complex, revealing that Tspan12 directly binds a Norrin dimer without enhancing its affinity for FZD4. Instead, Tspan12 acts as a ligand organizer, ...