Structural and molecular homogeneity of ATTRv-T60A amyloid fibrils across patients and organs [0.03%]
Maria Del Carmen Fernandez-Ramirez,Binh A Nguyen,Shumaila Afrin et al.
Maria Del Carmen Fernandez-Ramirez et al.
Transthyretin amyloidosis is a systemic protein misfolding disorder with diverse clinical phenotypes, including cardiomyopathy, polyneuropathy, or a combination of both. While structural polymorphism of amyloid fibrils has been linked to di...
Structures reveal how the Cas1-2/3 integrase captures, delivers, and integrates foreign DNA into CRISPR loci [0.03%]
结构揭示Cas1-2/3整合酶如何捕获、递送并将其DNA整合到CRISPR区域中
William S Henriques,Jarrett Bowman,Laina N Hall et al.
William S Henriques et al.
Cas1 and Cas2 are the hallmark proteins of prokaryotic adaptive immunity. However, these two proteins are often fused to other proteins and the functional association of these fusions often remain poorly understood. Here we purify and deter...
Structural basis for DCAF2 as a novel E3 ligase for PROTAC-mediated targeted protein degradation [0.03%]
DCAF2作为PROTAC介导的靶向蛋白质降解E3连接酶的结构基础
Evan J McMahon,Alexander G Cioffi,Patrick R Visperas et al.
Evan J McMahon et al.
Targeted protein degradation (TPD) leverages the ubiquitin-proteasome system to eliminate disease-causing proteins via E3 ligases. To date, the field is limited to utilizing a few of the over 600 human E3 ligases. To expand this repertoire,...
S M Naimul Hasan,Elnaz Khalili Samani,Alexander F A Keszei et al.
S M Naimul Hasan et al.
Mycobiota fatty acid synthases (FASs) catalyze iterative cycles of condensation, dehydration, and reduction to produce saturated fatty acids. Although these multienzymes are attractive antifungal drug targets, no clinically approved small-m...
Nikolaos Louros,Joost Schymkowitz,Frederic Rousseau
Nikolaos Louros
In this issue of Structure, Connor et al.1 combine topology and residue-level energetics of α-synuclein and tau fibrils to identify isoenergetic fold families. They reveal how conserved hotspot residues, mutations, and, potentially, enviro...
Fiyaz Mohammed,Carrie R Willcox,Benjamin E Willcox
Fiyaz Mohammed
In this issue of Structure, Rashleigh et al.1 used cryoelectron microscopy to assess recognition of the model foreign antigen phycoerythrin (PE) by a γδ TCR, an αβ TCR, and an antibody. With potential relevance for anti-pathogen immunit...
Proteasome-associated autoinflammatory syndromes: The impact of mutations in proteasome subunits on particle assembly, structure, and activity [0.03%]
蛋白酶体相关性自身炎症综合征:蛋白酶体亚基突变对颗粒组装、结构和活性的影响
Eva M Huber,Wolfgang Heinemeyer,Michael Groll
Eva M Huber
Single point mutations in proteasome subunits can cause severe autoinflammatory syndromes. By still largely unknown mechanisms, some of these disease-associated mutations impair normal proteasome function and induce the production of pro-in...
Lipids modulate the open probability of RyR1 under cryo-EM conditions [0.03%]
脂质可调节CRYO-EM条件下RyR1的开放概率
Chenyao Li,Rouslan G Efremov
Chenyao Li
Ryanodine receptors (RyRs) are intracellular tetrameric ion channels responsible for Ca2+ release from the sarcoplasmic and endoplasmic reticulum. Ryanodine receptor 1 (RyR1) isoform, critical for muscle contraction, has been studied most e...
Structural insights into IL-31 signaling inhibition by a neutralizing antibody [0.03%]
中和型抗体结合IL-31的分子机制研究
Tianling Guo,Yuxin Zheng,Zheng Fan et al.
Tianling Guo et al.
Interleukin-31 (IL-31) signals through the IL-31 receptor alpha (IL-31RA) and oncostatin M receptor beta (OSMRβ) heterodimer, mediating pruritus, dermatitis, inflammatory responses, neuroimmune interactions, and certain cancers. Here, we p...
Flexibility or uncertainty? A critical assessment of AlphaFold 2 pLDDT [0.03%]
灵活性还是不确定性?对AlphaFold 2 pLDDT的批判性评估
Yann Vander Meersche,Julien Diharce,Jean-Christophe Gelly et al.
Yann Vander Meersche et al.
Release of AlphaFold 2 and subsequent development of AlphaFold 3 had a profound impact on protein structure prediction, providing near-experimental accuracy. However, the utility of AF2's confidence index (pLDDT) as indicators of protein fl...